Abstract: Disclosed is a method for introducing a plurality of programmed nucleotide modifications into a single locus of a desired genomic DNA sequence in a single experiment. The method entails synthesizing a homology-directed repair (HDR) library comprising a plurality of oligonucleotides, wherein each oligonucleotide comprises a programmed nucleotide modification in the locus of the desired genome, and co-transfecting a population of cells with (i) an expression system capable of expressing Cas9 and a single guide RNA (sgRNA) and (ii) introducing a plurality of programmed nucleotide modifications to the locus of the desired genomic DNA sequence in one or more cells of the population. Also disclosed are methods for analyzing the functional consequence of a genomic mutation and for genomic screening.

Abstract: A system for producing a transcript of a meeting having n attendees, the attendees being identified as ID1 to IDn and channel 1 to channel n. A speech discriminator includes a channel monitor which generates a speech output from one or more of channels 1 to n at any one times a speech file selector at 14 and a speech file database at 15. Discrimination is on the basis of pre-allocated channels which correspond to pre-allocated microphones which are matched by ID and to the speech files in the speech file database. The effect of 13, 14 and 15 is to match a channel input to a particular speech file in the database 15 so that this information may then be passed to the audio to text convertor such that the speech file information and the input audio may be converted to text, displayed and written to a text file.