Patents by Inventor Gudrun Schmitz
Gudrun Schmitz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8008054Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: GrantFiled: June 3, 2010Date of Patent: August 30, 2011Assignee: Roche Molecular Systems, Inc.Inventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Publication number: 20110020898Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: ApplicationFiled: June 3, 2010Publication date: January 27, 2011Applicant: Roche Diagnostics GmBHInventors: Waltraud ANKENBAUER, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Patent number: 7759107Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: GrantFiled: June 9, 2008Date of Patent: July 20, 2010Assignee: Roche Diagnostics GmbHInventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Publication number: 20090093043Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: ApplicationFiled: June 9, 2008Publication date: April 9, 2009Applicant: Roche Diagnostics GmBHInventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Patent number: 7425423Abstract: A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).Type: GrantFiled: October 1, 1997Date of Patent: September 16, 2008Assignee: Roche Diagnostics GmbHInventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
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Publication number: 20060099596Abstract: The present invention is directed to a method for identification of a pathogenic organism from a predetermined group of pathogens, comprising (i) isolating a clinical sample containing at least partially purified nucleic acid, (ii) subjecting at least a first aliquot of said clinical specimen to at least one amplification and detection reaction in one reaction vessel comprising (iia) an amplification step using at least a first set of amplification primers capable of amplifying a preselected nucleic acid sequence region from several or all members of said predetermined group of pathogens, (iib) a detection step using at least 2, 3 or multiple hybridization reagents, said reagents together being capable of specifically detecting a pre-selected nucleic acid sequence region from all members of said group of pathogens, said detection step (iib) comprising steps monitoring hybridization of each of said hybridization reagents at a pre-selected temperature, said hybridization being indicative for at least the genusType: ApplicationFiled: December 2, 2003Publication date: May 11, 2006Applicant: Roche Molecular Systems, Inc.Inventors: Gerd Haberhausen, Thomas Emrich, Gregor Sagner, Martin Moczko, Gudrun Schmitz-Agheguian
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Publication number: 20050019789Abstract: A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophilum. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be native or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.Type: ApplicationFiled: February 2, 2004Publication date: January 27, 2005Applicant: Roche Diagnostics GMBHInventors: Waltraud Ankenbauer, Gudrun Schmitz-Agheguian, Elizaveta Bonch-Osmolovskaya, Vitaly Svetlichny, Ursula Markau, Bernhard Angerer, Astrid Reiser
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Patent number: 6692932Abstract: A thermostable enzyme is provided which is derived from the microorganism Anaerocellum thermophiluml. The enzyme has a molecular weight of 96 to 100 kDa, shows DNA polymerase activity and reverse transcriptase activity in the presence of magnesium ions. The enzyme may be native or recombinant, and may be used with selected primers and nucleoside triphosphates in a temperature cycling polymerase chain reaction on DNA or RNA as template with or without additional DNA polymerases as an enzyme mixture.Type: GrantFiled: June 10, 1999Date of Patent: February 17, 2004Inventors: Waltraud Ankenbauer, Gudrun Schmitz-Agheguian, Elizaveta Bonch-Osmolovskaya, Vitaly Svetlichny, Ursula Markau, Bernhard Angerer, Astrid Reiser
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Patent number: 6468775Abstract: A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3′-5′ exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.Type: GrantFiled: November 22, 1999Date of Patent: October 22, 2002Assignee: Roche Molecular Systems Inc.Inventors: Waltraud Ankenbauer, Ursula Markau, Vitaly Svetlichny, Gudrun Schmitz-Agheguian, Astrid Reiser, Bernhard Angerer, Christine Ebenbichler, Frank Laue, Elizaveta Bonch-Osmolovskaya
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Patent number: 5702888Abstract: For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound vie a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.Type: GrantFiled: January 11, 1995Date of Patent: December 30, 1997Assignee: Boehringer Mannheim GmbHInventors: Hans-Joachim Holtke, Rudolf Seibl, Gudrun Schmitz, Hans Robert Scholer, Christoph Kessler, Ralf Mattes
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Patent number: 5344757Abstract: For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound via a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.Type: GrantFiled: January 9, 1990Date of Patent: September 6, 1994Assignee: Boehringer Mannheim GmbHInventors: Hans-Joachim Holtke, Rudolf Seibl, Gudrun Schmitz, Hans R. Scholer, Christoph Kessler, Ralf Mattes
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Patent number: 5204238Abstract: The present invention provides a process for the reduction of non-specific star activities in the case of the specific cleavage of desoxyribonucleic acids by incubation with a restriction endonuclease in an appropriate buffer, wherein to the incubation batch is added an antibiotic which binds to the DNA on or near the star sequences of the enzyme but not within the specific recognition sequence of the restriction endonuclease.Type: GrantFiled: March 14, 1991Date of Patent: April 20, 1993Assignee: Boehringer Mannheim GmbHInventors: Frank Laue, Waltraud Ankenbauer, Gudrun Schmitz
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Patent number: 5153122Abstract: New type II restriction endonuclease MamI has recognition sequence: ##STR1## and a cleavage site indicated by the arrows. It is preferably obtained from microorganisms of the genus Microbacterium.Type: GrantFiled: July 25, 1990Date of Patent: October 6, 1992Assignee: Boehringer Mannheim GmbHInventors: Klaus Kaluza, Gudrun Schmitz, Michael Jarsch, Christoph Kessler
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Patent number: 5134069Abstract: The type III restriction endonuclease SgrAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Streptomyces. It can be used to recognize and cleave the double-stranded DNA sequence ##STR2## and its complementary sequence.Type: GrantFiled: November 16, 1990Date of Patent: July 28, 1992Assignee: Boehringer Mannheim GmbHInventors: Klaus Kaluza, Bruno Frey, Gudrun Schmitz-Agheguian, Michael Jarsch, Christoph Kessler
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Patent number: 5134068Abstract: Type II restriction endonuclease McrI is disclosed. This endonuclease has recognition sequence: ##STR1## wherein R is G or A and Y is C or T, and the cleavage site indicated by the arrows. The endonuclease is preferably obtained from organism of genus Micrococcus. It can be used to recognize and cleave double stranded DNA sequence:5'-CGRYCG-3'and its complementary sequence.Type: GrantFiled: November 16, 1990Date of Patent: July 28, 1992Assignee: Boehringer Mannheim GmbHInventors: Klaus Kaluza, Hans J. Hoeltke, Michael Jarsch, Gudrun Schmitz-Agheguian, Christoph Kessler
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Patent number: 5134067Abstract: The type II restriction endonuclease RleAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Rhizobium. It can be used to recognize and cleave the double-stranded DNA sequence CCCACA(N).sub.12/9 and its complementary sequence.Type: GrantFiled: November 16, 1990Date of Patent: July 28, 1992Assignee: Boehringer Mannheim GmbHInventors: Klaus Kaluza, Michael Jarsch, Gudrun Schmitz-Agheguian, Christoph Kessler
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Patent number: 4975376Abstract: The present invention provides a restriction endonuclease which recognizes palindromic sequences ##STR1## where C* is methylated, and cleaves these sequences at the position indicated by the arrows. This endonuclease is preferably from a microorganism of the genus Kluyvera. The present invention also provides a process for obtaining this new restriction endonuclease and a method for using the endonuclease.Type: GrantFiled: March 30, 1989Date of Patent: December 4, 1990Assignee: Boehringer Mannheim GmbHInventors: Bryan J. Bolton, Michael Jarsch, Gudrun Schmitz, Christoph Kessler
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Patent number: 4970149Abstract: The present invention provides a restriction endonuclease, characterized by the recognition sequence:5'-CTCTTCN.dwnarw.NNN-N-3'3'-GAGAAGN-NNN.uparw.N-5'and the cleavage position defined by the arrows. The present invention also provides a process for obtaining this new restriction endonuclease and a method for using the endonuclease.Type: GrantFiled: September 2, 1988Date of Patent: November 13, 1990Assignee: Boehringer Mannheim GmbHInventors: Bryan J. Bolton, Michael Jarsch, Gudrun Schmitz, Christoph Kessler