Abstract: The present invention relates to a method for the detection and quantification of lipoprotein(a) present in a fluid sample which method is not sensitive to the presence of plasminogen and which comprises contacting the fluid sample with anti-apo(a) antibody and forming a first immobilized complex of lipoprotein(a)/anti-apo(a) first antibody, contacting said first complex with anti-apo B second antibody and thereafter quantitating Lp(a) based on the amount of bound anti-apo B antibody. Immobilized rabbit anti-human apo(a) antibody can be employed as the first, capture, antibody and goat-anti-human apo B antibody as the second antibody. The amount of anti-apo B antibody bound is quantitated through binding of a third antibody/enzyme conjugate, e.g., a rabbit anti-goat antibody/alkaline phosphatase conjugate, followed by reaction with a suitable enzyme substrate such as paranitrophenyl phosphate.