Patents by Inventor Guobing Xiang
Guobing Xiang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10941439Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.Type: GrantFiled: October 31, 2018Date of Patent: March 9, 2021Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Steven M. Menchen, Guobing Xiang, Prasanna Thwar, Jeffrey Frediani, Allen Wong, Alfred Lui, Lily Lu
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Patent number: 10676790Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.Type: GrantFiled: November 30, 2018Date of Patent: June 9, 2020Assignees: LIFE TECHNOLOGIES CORPORATION, LIFE TECHNOLOGIES ASInventors: Geir Fonnum, Steven M. Menchen, Alfred Lui, Guobing Xiang, Synne Larsen
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Publication number: 20190177787Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.Type: ApplicationFiled: November 30, 2018Publication date: June 13, 2019Inventors: Geir FONNUM, Steven M. MENCHEN, Alfred LUI, Guobing XIANG, Synne LARSEN
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Publication number: 20190062807Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.Type: ApplicationFiled: October 31, 2018Publication date: February 28, 2019Inventors: Steven M. Menchen, Guobing Xiang, Prasanna Thwar, Jeffrey Frediani, Allen Wong, Alfred Lui, Lily Lu
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Patent number: 10150992Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.Type: GrantFiled: July 5, 2016Date of Patent: December 11, 2018Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Steven M. Menchen, Guobing Xiang, Prasanna Krishnan Thwar, Jeffrey Frediani, Allen Wong, Alfred Lui, Lily Lu
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Patent number: 10144968Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.Type: GrantFiled: July 1, 2016Date of Patent: December 4, 2018Assignees: LIFE TECHNOLOGIES CORPORATION, LIFE TECHNOLOGIES ASInventors: Geir Fonnum, Steven M. Menchen, Alfred Lui, Guobing Xiang, Synne Larsen
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Publication number: 20170009275Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.Type: ApplicationFiled: July 5, 2016Publication date: January 12, 2017Inventors: Steven M. MENCHEN, Guobing XIANG, Prasanna Krishnan THWAR, Jeffrey FREDIANI, Allen WONG, Alfred LUI, Lily LU
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Publication number: 20170002410Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.Type: ApplicationFiled: July 1, 2016Publication date: January 5, 2017Inventors: Geir Fonnum, Steven M. Menchen, Alfred Lui, Guobing Xiang, Synne Larsen
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Publication number: 20090023150Abstract: Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.Type: ApplicationFiled: June 27, 2008Publication date: January 22, 2009Applicant: SEQUENOM, INC.Inventors: Hubert Koster, Daniel P. Little, Andreas Braun, David M. Lough, Guobing Xiang, Dirk van den Boom, Christian Jurinke, Andreas Ruppert
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Publication number: 20050226816Abstract: The present invention relates generally to the detection and measurement of transmembrane potentials using an asymmetric thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a moderately hydrophobic asymmetric thiobarbituric acid-derived polymethine oxonols. The method comprises a moderately hydrophobic asymmetric thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes.Type: ApplicationFiled: October 21, 2004Publication date: October 13, 2005Applicant: AnaSpec, Inc.Inventors: Zhenjun Diwu, Yong Yao, Jianjun He, Guobing Xiang
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Publication number: 20020042112Abstract: Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.Type: ApplicationFiled: October 26, 1998Publication date: April 11, 2002Inventors: HUBERT KOSTER, DANIEL P. LITTLE, ANDREAS BRAUN, DAVID M. LOUGH, GUOBING XIANG, DIRK VAN DEN BOOM, CHRISTIAN JURINKE, ANDREAS RUPPERT
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Patent number: 6268131Abstract: A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods use a modified Sanger sequencing strategy in which RNA polymerase is used to generate a set of nested RNA transcripts obtained by base-specific chain termination. These are analyzed by mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.Type: GrantFiled: December 15, 1997Date of Patent: July 31, 2001Assignee: Sequenom, Inc.Inventors: Changwon Kang, Young-Soo Kwon, Young Tae Kim, Hubert Köster, Daniel P. Little, Maryanne J. Little, Guobing Xiang, David M. Lough, Charles Cantor