Abstract: Methods of preparing surfaces of sample wells are provided. In some aspects, methods of preparing a sample well surface involve contacting the sample well with a block copolymer to form an antifouling overlay over a metal oxide surface of the sample well. In some aspects, methods of passivating and/or selectively functionalizing a sample well surface are provided.

Abstract: Provided are a unimolecular nanoparticle, a composition thereof, and methods of use thereof, and includes 1) a dendritic polymer having a molecular weight of about 500-120,000 Da and terminating in hydroxyl, amino or carboxylic acid groups; 2) cationic polymers attached to at least a majority of the terminating groups of the dendritic polymer via a pH-sensitive linker, wherein each cationic polymer comprises a polymeric backbone attached to cationic functional groups and to weakly basic groups by disulfide bonds, wherein the molar ratio of cationic functional groups to weakly basic groups ranges from 1:1-5:1, and has a molecular weight from about 1,000-5,000 Da; and 3) poly(ethylene glycol) attached to a plurality of cationic polymers and having a terminal group selected from a targeting ligand, OH, O-alkyl, NH2 , biotin, or a dye, wherein the terminal group of at least one poly(ethylene glycol) is having a molecular weight of about 1,000-15,000 Da.

Abstract: Implementations of the subject matter described herein relate to mixed reality rendering of objects. According to the embodiments of the subject matter described herein, while rendering an object, a wearable computing device takes lighting conditions in the real world into account, thereby increasing the reality of the rendered object. In particular, the wearable computing device acquires environment lighting information of an object to be rendered and renders the object to a user based on the environment lighting information. In this way, the object rendered by the wearable computing device can be more real and accurate. The user will thus have a better interaction experience.

Abstract: Embodiments of the invention provide a workbench, includes: a workbench unit (110), including a first workbench unit (112) and a second workbench unit (114), the first workbench unit (112) and the second workbench unit (114) are rotatably connected; the first table unit (112) is provided with a first working surface (1120), and the second workbench unit (114) is provided with a second working surface (1140); a bracket (120), including a plurality of support legs (122) and detachably connected to the workbench unit (110); when the workbench unit (110) is in the first state, the first working surface (1120) and the second working surface (1140) together form a working surface supporting the workpiece; when the workbench unit (110) is in the second state, the first workbench unit (112) and the second workbench unit (114) form a bracket placement cavity (140) for at least partially accommodating the bracket (120).

Abstract: In accordance with implementations of the subject matter described herein, there is provided a solution of lighting estimation. In the solution, an input image about a real object and a depth map corresponding to the input image are obtained. A geometric structure of the scene in the input image is determined based on the depth map. Shading and shadow information on the real object caused by a light source in the scene is determined based on the determined geometric structure of the scene. Then, a lighting condition in the scene caused by the light source is determined based on the input image and the shading and shadow information. The virtual object rendered using the lighting condition obtained according to the solution can exhibit a realistic effect consistent with the real object.

Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.

Abstract: The present invention provides a method for attaching cells to a biosensor surface (5) of a biosensor (4) comprising: providing a cell suspension in a liquid receiving unit (1), wherein the cell suspension forms a surface (7) to the exterior of the liquid receiving unit (1); contacting the biosensor surface (5) with the surface (7) of the cell suspension in the liquid receiving unit (1); and allowing the cells to settle on the biosensor surface (5) by gravity, and allowing the cells to adhere to the biosensor surface (5).

Abstract: Disclosed are compositions and methods for microneedle patches comprising copolymer designed for glucose triggered insulin delivery. In one aspect, disclosed herein are microneedle patches comprising insulin loaded copolymers; wherein the insulin dissociates from the microneedle in an hyperglycemic environment; wherein the copolymer comprises poly(N-vinylpyrrolidone-co-2-(dimethylamino)ethyl acrylate-co-3-(acrylamido)phenylboronic acid and methods of their use.

Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.

Abstract: Provided are a unimolecular nanoparticle, a composition thereof, and methods of use thereof, and includes 1) a dendritic polymer having a molecular weight of about 500-120,000 Da and terminating in hydroxyl, amino or carboxylic acid groups; 2) cationic polymers attached to at least a majority of the terminating groups of the dendritic polymer via a pH-sensitive linker, wherein each cationic polymer comprises a polymeric backbone attached to cationic functional groups and to weakly basic groups by disulfide bonds, wherein the molar ratio of cationic functional groups to weakly basic groups ranges from 1:1-5:1, and has a molecular weight from about 1,000-5,000 Da; and 3) poly(ethylene glycol) attached to a plurality of cationic polymers and having a terminal group selected from a targeting ligand, OH, O-alkyl, NH2, biotin, or a dye, wherein the terminal group of at least one poly(ethylene glycol) is having a molecular weight of about 1,000-15,000 Da.

Abstract: The present disclosure relates to unimolecular core-shell nanoparticle, nanoclusters thereof, and platelet biomimetic nanoclusters thereof. The disclosed compositions are useful for treating a subject with a disease or condition, such as a cardiovascular disease. In a further aspect, the cardiovascular disease can be a vascular stenosis or restenosis. Also described herein are methods of making and using the unimolecular core-shell nanoparticle, nanoclusters thereof, and platelet biomimetic nanoclusters thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Abstract: Provided herein are nanocapsules comprising a single ribonucleoprotein (RNP) complex as a core and an biodegradable crosslinked polymer shell that encapsulates the core, wherein the RNP complex comprises a Cas9 polypeptide and a guide RNA, and the biodegradable crosslinked polymer shell comprises polymerized monomers of imidazolyl acryloyl monomers, bisacryloyl disulfide monomers (a biodegradable cross-linker), optionally PEG acryloyl monomers, and either cationic acryloyl monomers, anionic acryloyl monomers, or both cationic and anionic acryloyl monomers (optionally in combination with non-ionic acryloyl monomers) as defined herein. Also provided are methods of making the nanocapsules, kits containing the nanocapsules and methods of delivering the encapsulated RNP to cells.

Abstract: Aspects of the application provide methods of producing substrates having modified surfaces. In some aspects, methods of surface modification involve treating a surface of a substrate with an organic reagent in vapor phase to form an organic layer over the surface. In some aspects, methods of forming a stable surface coating on an oxidized surface are provided.

Abstract: Implementations of the subject matter described herein relate to mixed reality object rendering based on ambient light conditions. According to the embodiments of the subject matter described herein, while rendering an object, a wearable computing device acquires light conditions of the real world, thereby increasing the reality of the rendered object. In particular, the wearable computing device is configured to acquire an image of an environment where the wearable computing device is located. The image is adjusted based on a camera parameter used when the image is captured. Subsequently, ambient light information is determined based on the adjusted image. In this way, the wearable computing device can obtain more real and accurate ambient light information, so as to render to the user an object with enhanced reality. Accordingly, the user can have a better interaction experience.

Abstract: A nanopore cell may include a well having a working electrode at a bottom of the well. The well may be formed within a dielectric layer, where the sidewalls of the well may be formed of the dielectric or other materials coating the walls of the dielectric layer. Various materials having different hydrophobicity and hydrophilicity can be used to provide desired properties of the cell. The nanopore in the nanopore cell can be inserted in a membrane formed over the well. Various techniques can be used for providing a desired shape and other properties e.g., of materials and processes for forming the well.

Abstract: In accordance with implementations of the subject matter described herein, there is provided a solution of lighting estimation. In the solution, an input image about a real object and a depth map corresponding to the input image are obtained. A geometric structure of the scene in the input image is determined based on the depth map. Shading and shadow information on the real object caused by a light source in the scene is determined based on the determined geometric structure of the scene. Then, a lighting condition in the scene caused by the light source is determined based on the input image and the shading and shadow information. The virtual object rendered using the lighting condition obtained according to the solution can exhibit a realistic effect consistent with the real object.

Abstract: A nanopore cell may include a well having a working electrode at a bottom of the well. The well may be formed within a dielectric layer, where the sidewalls of the well may be formed of the dielectric or other materials coating the walls of the dielectric layer. Various materials having different hydrophobicity and hydrophilicity can be used to provide desired properties of the cell. The nanopore in the nanopore cell can be inserted in a membrane formed over the well. Various techniques can be used for providing a desired shape and other properties e.g., of materials and processes for forming the well.

Abstract: A method of forming an integrated device includes forming a sample well within a cladding layer of a substrate; forming a sacrificial spacer layer over the substrate and into the sample well; performing a directional etch of the sacrificial spacer layer so as to form a sacrificial sidewall spacer on sidewalls of the sample well; forming, over the substrate and into the sample well, a functional layer that provides a location for attachment of a biomolecule; and removing the sacrificial spacer material.

Abstract: The present technology provide compositions that are drug delivery systems for the sustained release of anti-stenotic drugs for the treatment and prevention of occlusion of blood vessels, particularly after perivascular surgery. The compositions include a hydrogel, unimolecular micelles dispersed within the hydrogel, and an effective amount of anti-stenotic drug dispersed within the unimolecular micelle. The hydrogel may be a di-or tri-block copolymer comprising one block of poly(ethylene glycol) (PEG) and one or two blocks of poly(lactic-co-glycolic acid) (PLGA). The unimolecular micelle may include three domains: a dendritic polymer core, hydrophobic block polymers (e.g., PVL, PVCL, and/or PCL) attached to the core and PEG attached to the hydrophobic block polymers.

Abstract: A dust bin and a vacuum cleaner combination provided with the dust bin are provided. The dust bin includes a dust chamber, a dust inlet detachably connected to a dust suction apparatus, and a sealing structure for implementing the sealing between the dust suction apparatus and the dust bin. The dust suction apparatus has a dust outlet joined to the dust inlet. The vacuum cleaner combination includes a dust suction apparatus and a dust bin detachably connected to the dust suction apparatus. The dust suction apparatus has a housing and a dust cup assembly connected to the housing. The dust cup assembly includes a cup body and a filter apparatus disposed in the cup body. Compared with the prior art, in the present invention, a detachable multi-purpose dust bin with a simple structure is disposed, so that the dust collection chamber of the vacuum cleaner is flexibly increased.