Abstract: Provided is an integrated microfluidic device for SARS-CoV-2 detection. Also provided is a method for detecting SARS-CoV-2 by using the same, comprising viral lysis, RNA extraction, and reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The integrated microfluidic device of the present disclosure is small in size, automatically operatable, and easy to use by ordinary people, and the present disclosure can achieve rapid detection with high sensitivity and specificity.

Abstract: The present disclosure provides a method for detecting cholangiocarcinoma cells. The capture rate of the cholangiocarcinoma cells of the present disclosure is higher than 70%, and a plurality of octasaccharides with high affinity and specificity can be modified on the surface of magnetic beads to capture and analyze cholangiocarcinoma cells under test, wherein the cholangiocarcinoma cells can be circulating tumor cells in cholangiocarcinoma.

Abstract: Disclosed herein is a novel compound of formula (I) for detecting circulating cancerous cells, particularly, cholangio-cancerous cells, from a biological sample, wherein, R1 and R2 are independently H, or —SO3M; and M is a monovalent cation selected from the group consisting of sodium ion, potassium ion, lithium ion or ammonium ion. Also disclosed herein is a method of treating and detecting cholangio-cancerous cells from a biological sample of a subject suspected of having cholangiocarcinoma (CCA). The method includes steps of, contacting the biological sample with a magnetic bead pre-coated with the compound of formula (I); detecting a complex formed between the magnetic bead and the biological sample in an immunoassay; and administering to the subject an effective amount of a chemotherapeutic agent to ameliorate symptoms associated with the CCA.

Abstract: Disclosed herein is a novel compound of formula (I) for detecting circulating cancerous cells, particularly, cholangio-cancerous cells, from a biological sample, wherein, R1 and R2 are independently H, or —SO3M; and M is a monovalent cation selected from the group consisting of sodium ion, potassium ion, lithium ion or ammonium ion. Also disclosed herein is a method of treating and detecting cholangio-cancerous cells from a biological sample of a subject suspected of having cholangiocarcinoma (CCA). The method includes steps of, contacting the biological sample with a magnetic bead pre-coated with the compound of formula (I); detecting a complex formed between the magnetic bead and the biological sample in an immunoassay; and administering to the subject an effective amount of a chemotherapeutic agent to ameliorate symptoms associated with the CCA.

Abstract: The present disclosure provides a method for detecting live Mycobacterium tuberculosis in a sample. The method of the present disclosure can rapidly detect Mycobacterium tuberculosis, identify live Mycobacterium tuberculosis, greatly reduce the required reagents and biological samples, and simplify the experimental procedure and time.

Abstract: The present disclosure provides an automatic microfluidic system for rapid personalized drug screening including a microfluidic chip. The microfluidic chip includes a fluid storage unit, a fluid driving unit, a reaction unit and a plurality of valve units. The fluid driving unit includes two mixing pumps. Each of the mixing pumps includes two pneumatic micro-pumps, a mixing chamber and a blocking structure. The blocking structure is disposed in the mixing chamber and is connected between the two pneumatic micro-pumps. When the two pneumatic micro-pumps are started alternately, the blocking structure is deflected alone with the operation of the two pneumatic micro-pumps.

Abstract: Disclosed herein is an integrated microfluidic chip for detecting cancerous cells, particularly, cholangio-cancerous cells, from a biological sample. Also disclosed herein is a method of detecting cholangio-cancerous cells from a biological sample.

Abstract: The present disclosure provides a method for detecting cholangiocarcinoma cells. The capture rate of the cholangiocarcinoma cells of the present disclosure is higher than 70%, and a plurality of octasaccharides with high affinity and specificity can be modified on the surface of magnetic beads to capture and analyze cholangiocarcinoma cells under test, wherein the cholangiocarcinoma cells can be circulating tumor cells in cholangiocarcinoma.

Abstract: The present disclosure relates to a test kit for detecting a concentration of a cardiovascular disease-related biomarker including a group selected from the group consisting of a first aptamer, a second aptamer and a third aptamer. The first aptamer has binding specificity to an N-terminal pro-brain natriuretic peptide (NT-proBNP). The second aptamer has binding specificity to a cardiac Troponin I (cTnI). The third aptamer has binding specificity to a fibrinogen.

Abstract: The present disclosure relates to a test kit for detecting a concentration of a cardiovascular disease-related biomarker including a group selected from the group consisting of a first aptamer, a second aptamer and a third aptamer. The first aptamer has binding specificity to an N-terminal pro-brain natriuretic peptide (NT-proBNP). The second aptamer has binding specificity to a cardiac Troponin I (cTnI). The third aptamer has binding specificity to a fibrinogen.

Abstract: The present disclosure provides an automatic microfluidic system for rapid personalized drug screening including a microfluidic chip. The microfluidic chip includes a fluid storage unit, a fluid driving unit, a reaction unit and a plurality of valve units. The fluid driving unit includes two mixing pumps. Each of the mixing pumps includes two pneumatic micro-pumps, a mixing chamber and a blocking structure. The blocking structure is disposed in the mixing chamber and is connected between the two pneumatic micro-pumps. When the two pneumatic micro-pumps are started alternately, the blocking structure is deflected alone with the operation of the two pneumatic micro-pumps.

Abstract: A self-driven microfluidic chip for rapid influenza A detection is provided. The chip includes: a substrate, a hydrophobic layer, a hydrophilic film layer, and a channel structure layer laminated sequentially. The structure of the channel structure layer includes a plurality of channels, a plurality of valves and reaction chambers in the channels, and a plurality of openings, wherein the hydrophilic film layer includes a pattern corresponding to the structure of the channel structure layer, and forms a disconnected area corresponding to the location of the valves to make the valves hydrophobic; the channel structure layer is formed of a flexible material, and heights of the valves are higher than those of the channels in a thickness direction of the channel structure layer in order to control liquid flow by pressing the valves.

Abstract: An automatic microfluidic system for antibiotic susceptibility testing of the present disclosure at least includes a microfluidic chip. The microfluidic chip includes a fluid storage unit, a reaction unit, a pneumatic micro-pumping unit and a plurality of valve units. The fluid storage unit is provided for storing a bacterial suspension, a broth and an antibiotic solution. The reaction unit includes a first reaction chamber and at least two second reaction chambers. The pneumatic micro-pumping unit is adjacently disposed to the fluid storage unit and the reaction unit for selectively, repeatedly and quantitatively transporting the broth, the bacterial suspension and the antibiotic solution to the reaction unit to form a first mixing solution and at least two second mixing solutions. The valve units include a plurality of pneumatic micro-valves and a plurality of valve control air holes for controlling the opening and closing of the pneumatic micro-valves.

Abstract: The present disclosure provides a method for detecting live Mycobacterium tuberculosis in a sample. The method of the present disclosure can rapidly detect Mycobacterium tuberculosis, identify live Mycobacterium tuberculosis, greatly reduce the required reagents and biological samples, and simplify the experimental procedure and time.

Abstract: An aptamer specific to ovarian cancer and a detection method for ovarian cancer are provided. The aptamer includes a following nucleotide sequence: 5?-ncaaannncnnnnanncnnnnnnnnnnngaannnannngg-3?, wherein n is a nucleotide independently selected from “a,” “t,” “c,” and “g.

Abstract: The present disclosure relates to an oligopeptide which is highly specific to an ovarian cancer. The oligopeptide includes an amino acid sequence of SEQ ID NO: 1. The present disclosure also relates to a test kit including the oligopeptide for detecting ovarian cancer and an ovarian cancer detection method by using the oligopeptide.

Abstract: A method for analyzing concentration of a cardiovascular disease (CVD) biomarker in a liquid sample includes: applying the liquid sample to a biosensor, the biosensor including a transistor having a drain, a source, and a gate terminal disposed between the gate and the source, and a reactive electrode spaced apart from the gate terminal of the transistor and having a receptor immobilized thereon for specific binding with the CVD biomarker, the liquid sample being in contact with the gate terminal and the reactive electrode; applying a voltage pulse between the reactive electrode and the source, the voltage pulse having a pulse width; monitoring a response current in response to the voltage pulse; and analyzing the response current.

Abstract: An automatic microfluidic system for antibiotic susceptibility testing of the present disclosure at least includes a microfluidic chip. The microfluidic chip includes a fluid storage unit, a reaction unit, a pneumatic micro-pumping unit and a plurality of valve units. The fluid storage unit is provided for storing a bacterial suspension, a broth and an antibiotic solution. The reaction unit includes a first reaction chamber and at least two second reaction chambers. The pneumatic micro-pumping unit is adjacently disposed to the fluid storage unit and the reaction unit for selectively, repeatedly and quantitatively transporting the broth, the bacterial suspension and the antibiotic solution to the reaction unit to form a first mixing solution and at least two second mixing solutions. The valve units include a plurality of pneumatic micro-valves and a plurality of valve control air holes for controlling the opening and closing of the pneumatic micro-valves.

Abstract: A microfluidic in-vitro screening chip system including a first micromixer chamber, a plurality of first storage chambers, a second micromixer chamber and a plurality of second storage chambers. The first micromixer chamber includes a non-target disease tissue slide region. The plurality of first storage chambers are connected to the first micromixer chamber, wherein at least one first storage chamber is used for storing a library. The second micromixer chamber is connected to the first micromixer chamber, and the second micromixer chamber includes a target disease tissue slide region. The plurality of second storage chambers are connected to the second micromixer chamber.

Abstract: An aptamer specific to ovarian cancer and a detection method for ovarian cancer are provided. The aptamer includes a following nucleotide sequence: 5?-ncaaannncnnnnanncnnnimnnnnngaannnannngg-3?, wherein n is a nucleotide independently selected from “a,” “t,” “c,” and “g.