Patents by Inventor Gyorgy Posfai
Gyorgy Posfai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10479820Abstract: Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.Type: GrantFiled: March 2, 2015Date of Patent: November 19, 2019Assignee: SCARAB GENOMICS, LLCInventors: Cristopher R. Blattner, David Frisch, Robert E. Novy, Terrance M. Henker, Eric A. Steffen, Frederick R. Blattner, Hyunsic Choi, Gyorgy Posfai, Charles F. Landry
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Publication number: 20180208933Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.Type: ApplicationFiled: February 23, 2018Publication date: July 26, 2018Applicant: SCARAB GENOMICS, LLCInventors: Frederick R. BLATTNER, David A. FRISCH, Robert E. NOVY, Terrance M. HENKER, Eric A. STEFFEN, Jose A. SANCHEZ, Christopher R. BLATTNER, Hyunsic CHOI, Gyorgy POSFAI
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Patent number: 9914905Abstract: Reduced genome bacteria with improved genetic stability are provided. Also provided are methods of producing polypeptides using the reduced genome bacteria with improved genetic stability.Type: GrantFiled: December 29, 2016Date of Patent: March 13, 2018Assignee: SCARAB GENOMICS, LLCInventors: Frederick R. Blattner, Balint Csorgo, Gyorgy Posfai
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Patent number: 9902965Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.Type: GrantFiled: November 13, 2014Date of Patent: February 27, 2018Assignee: SCARAB GENOMICS, LLCInventors: Frederick R. Blattner, David A. Frisch, Robert E. Novy, Terrance M. Henker, Eric A. Steffen, Jose A. Sanchez, Christopher R. Blattner, Hyunsic Choi, Gyorgy Posfai
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Publication number: 20170114318Abstract: Reduced genome bacteria with improved genetic stability are provided. Also provided are methods of producing polypeptides using the reduced genome bacteria with improved genetic stability.Type: ApplicationFiled: December 29, 2016Publication date: April 27, 2017Inventors: Frederick R. BLATTNER, Balint CSORGO, Gyorgy POSFAI
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Publication number: 20170073379Abstract: Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.Type: ApplicationFiled: March 2, 2015Publication date: March 16, 2017Applicant: SCARAB GENOMICS, LLCInventors: Cristopher R. BLATTNER, David FRISCH, Robert E. NOVY, Terrance M. HENKER, Eric A. STEFFEN, Frederick R. BLATTNER, Hyunsic CHOI, Gyorgy Posfai, Charles F. LANDRY
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Publication number: 20160298126Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.Type: ApplicationFiled: November 13, 2014Publication date: October 13, 2016Applicant: SCARAB GENOMICS, LLCInventors: Frederick R. BLATTNER, David A. FRISCH, Robert E. NOVY, Terrance M. HENKER, Eric A. STEFFEN, Jose A. SANCHEZ, Christopher R. BLATTNER, Hyunsic CHOI, Gyorgy POSFAI
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Patent number: 9340791Abstract: Reduced genome bacteria with improved genetic stability are provided. Also provided are methods of producing polypeptides using the reduced genome bacteria with improved genetic stability.Type: GrantFiled: October 19, 2012Date of Patent: May 17, 2016Assignee: SCARAB GENOMICS, LLCInventors: Frederick R. Blattner, Balint Csorgo, Gyorgy Posfai
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Publication number: 20150344838Abstract: Reduced genome strains of E. coli MG1 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.Type: ApplicationFiled: July 20, 2015Publication date: December 3, 2015Applicant: SCARAB GENOMICS, LLCInventors: John W. Campbell, Frederick R. Blattner, Guy Plunkett, Gyorgy Posfai
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Patent number: 9085765Abstract: Reduced genome strains of E. coli MGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.Type: GrantFiled: April 12, 2012Date of Patent: July 21, 2015Assignee: SCARAB GENOMICS LLCInventors: John W. Campbell, Frederick R. Blattner, Guy Plunkett, Gyorgy Posfai
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Publication number: 20140302555Abstract: Reduced genome bacteria with improved genetic stability are provided. Also provided are methods of producing polypeptides using the reduced genome bacteria with improved genetic stability.Type: ApplicationFiled: October 19, 2012Publication date: October 9, 2014Applicant: SCARAB GENOMICS, LLCInventors: Frederick R. Blattner, Balint Csorgo, Gyorgy Posfai
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Patent number: 8765408Abstract: The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.Type: GrantFiled: February 1, 2012Date of Patent: July 1, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Frederick R. Blattner, John W. Campbell, David Frisch, Guy Plunkett, III, Gyorgy Posfai
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Publication number: 20130011874Abstract: Reduced genome strains of E. coli MG1 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.Type: ApplicationFiled: April 12, 2012Publication date: January 10, 2013Applicant: SCARAB GENOMICS, LLCInventors: John W. Campbell, Frederick R. Blattner, Guy Plunkett, Gyorgy Posfai
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Publication number: 20120219994Abstract: The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.Type: ApplicationFiled: February 1, 2012Publication date: August 30, 2012Inventors: FREDERICK R. BLATTNER, John W. Campbell, David Frisch, Guy Plunkett, III, Gyorgy Posfai
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Patent number: 8178339Abstract: Reduced genome strains of E. coli MG1655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.Type: GrantFiled: August 18, 2006Date of Patent: May 15, 2012Assignee: Scarab Genomics LLCInventors: John W Campbell, Frederick R. Blattner, Guy Plunkett, Gyorgy Posfai
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Patent number: 8119365Abstract: A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.Type: GrantFiled: April 7, 2006Date of Patent: February 21, 2012Assignee: Wisconsin Alumni Research FoundationInventors: Frederick R. Blattner, John W. Campbell, David Frisch, Guy Plunkett, Gyorgy Posfai
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Patent number: 8043842Abstract: The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to 14% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.Type: GrantFiled: July 1, 2005Date of Patent: October 25, 2011Assignee: Wisconsin Alumni Research FoundationInventors: Frederick R. Blattner, Gyorgy Posfai, Christopher D. Herring, Guy Plunkett, Jeremy D. Glasner
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Publication number: 20090075333Abstract: Reduced genome strains of E. coli MGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.Type: ApplicationFiled: August 18, 2006Publication date: March 19, 2009Inventors: John W. Campbell, Frederick R. Blattner, Guy Plunkett, Gyorgy Posfai
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Patent number: 7303906Abstract: The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specific E. coli strains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.Type: GrantFiled: September 5, 2003Date of Patent: December 4, 2007Assignees: Wisconsin Alumni Research Foundation, Scarab Genomics, LLCInventors: Frederick R. Blattner, Gyorgy Posfai, Christopher D. Herring, Guy Plunkett, III, Jeremy Glasner, Trevor Twose
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Publication number: 20070054358Abstract: A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.Type: ApplicationFiled: April 7, 2006Publication date: March 8, 2007Inventors: Frederick Blattner, John Campbell, David Frisch, Guy Plunkett, Gyorgy Posfai