Patents by Inventor H. Pin Kao
H. Pin Kao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150238919Abstract: Exemplary embodiments provide microfluidic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.Type: ApplicationFiled: January 30, 2015Publication date: August 27, 2015Inventors: Mark F. OLDHAM, Dar BAHATT, Kenneth J. LIVAK, Jason E. BABCOKE, H. Pin KAO, Stephen J. GUNSTREAM, Kevin S. BODNER, Douglas P. GREINER, Nigel P. BEARD
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Publication number: 20150102025Abstract: A heating apparatus comprising a support base and a microplate having a first surface and an opposing second surface. The microplate is positioned adjacent the support base and comprises a plurality of wells formed in the first surface thereof. Each of the plurality of wells is sized to receive an assay therein. A sapphire crystalline transparent window is positioned adjacent the microplate opposing the support base. A heating device heats the transparent window in response to a control system.Type: ApplicationFiled: September 4, 2014Publication date: April 16, 2015Inventors: Kirk M. HIRANO, Patrick Kinney, Douwe Haga, Jason Babcoke, Albert Carrillo, H. Pin Kao, James Nurse
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Patent number: 8945481Abstract: Exemplary embodiments provide microfludic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.Type: GrantFiled: June 24, 2008Date of Patent: February 3, 2015Assignee: Applied Biosystems, LLCInventors: Mark F. Oldham, Kenneth J. Livak, Jason E. Babcoke, H. Pin Kao, Stephen J. Gunstream, Kevin S. Bodner, Douglas P. Greiner, Nigel P. Beard, Dar Bahatt
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Publication number: 20140374407Abstract: A heating apparatus comprising a support base and a microplate having a first surface and an opposing second surface. The microplate is positioned adjacent the support base and comprises a plurality of wells formed in the first surface thereof. Each of the plurality of wells is sized to receive an assay therein. A sapphire crystalline transparent window is positioned adjacent the microplate opposing the support base. A heating device heats the transparent window in response to a control system.Type: ApplicationFiled: May 20, 2014Publication date: December 25, 2014Applicant: Applied Biosystems, LLCInventors: Kirk HIRANO, Patrick Kinney, Douwe Haga, Jason Babcoke, Albert Carrillo, H. Pin Kao, James Nurse
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Publication number: 20140238976Abstract: A heating apparatus comprising a support base and a microplate having a first surface and an opposing second surface. The microplate is positioned adjacent the support base and comprises a plurality of wells formed in the first surface thereof. Each of the plurality of wells is sized to receive an assay therein. A sapphire crystalline transparent window is positioned adjacent the microplate opposing the support base. A heating device heats the transparent window in response to a control system.Type: ApplicationFiled: February 3, 2014Publication date: August 28, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Kirk HIRANO, Patrick KINNEY, Douwe HAGA, Jason BABCOKE, Albert CARRILLO, H. Pin KAO, James NURSE
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Patent number: 8638509Abstract: A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.Type: GrantFiled: October 3, 2011Date of Patent: January 28, 2014Assignee: Applied Biosystems, LLCInventors: Barry Blasenheim, H. Pin Kao, Mark Oldham
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Publication number: 20120080610Abstract: A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.Type: ApplicationFiled: October 3, 2011Publication date: April 5, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Barry J. BLASENHEIM, H. Pin Kao, Mark F. Oldham
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Patent number: 8089623Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.Type: GrantFiled: August 18, 2009Date of Patent: January 3, 2012Assignee: Applied Biosystems LLCInventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
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Patent number: 8040619Abstract: A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.Type: GrantFiled: August 3, 2009Date of Patent: October 18, 2011Assignee: Applied Biosystems, LLCInventors: Barry J. Blasenheim, H. Pin Kao, Mark F. Oldham
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Publication number: 20100220321Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.Type: ApplicationFiled: August 18, 2009Publication date: September 2, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
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Publication number: 20100193672Abstract: A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.Type: ApplicationFiled: August 3, 2009Publication date: August 5, 2010Applicant: Life Technologies CorporationInventors: Barry J. Blasenheim, H. Pin Kao, Mark F. Oldham
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Publication number: 20100113285Abstract: A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample.Type: ApplicationFiled: April 28, 2009Publication date: May 6, 2010Applicant: Life Technologies CorporationInventors: H. Pin Kao, Ian A. Harding, Kathleen C. Hayashibara, Achim Karger, Fiona C. Laird Hyland, Mark F. Oldham, Omead Ostadan, Mark R. Pratt, Junko F. Stevens, Austin B. Tomaney, Greg Young
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Patent number: 7586600Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.Type: GrantFiled: July 21, 2008Date of Patent: September 8, 2009Assignee: Applied Biosystem, LLCInventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
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Patent number: 7570443Abstract: A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.Type: GrantFiled: March 22, 2005Date of Patent: August 4, 2009Assignee: Applied Biosystems, LLCInventors: Barry J. Blasenheim, H. Pin Kao, Mark F. Oldham
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Publication number: 20090176661Abstract: A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.Type: ApplicationFiled: March 19, 2009Publication date: July 9, 2009Applicant: APPLERA CORPORATIONInventors: Ian A. Harding, Donald Sandell, Kevin Bodner, Tim Woudenberg, Mark Oldham, H. Pin Kao, Gary Lim, John P. Bodeau, Adrian Fawcett, Robert C. Jones
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Publication number: 20080288179Abstract: Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.Type: ApplicationFiled: July 21, 2008Publication date: November 20, 2008Inventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
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Publication number: 20080213755Abstract: A device (420) and method for real-time amplification and detection of a large number of target nucleic acids contained in a large number of biological samples is provided. The device (420) can comprise a substrate (422) and an optically transparent cover, wherein the substrate can comprise a first surface, at least one sample receiving chamber (430), a distributor channel (434), at least one reaction chamber (428), and a vent (440) for each reaction chamber. The substrate can comprise a material having a thermal conductivity of at least about 0.25 W/m° K.Type: ApplicationFiled: January 12, 2005Publication date: September 4, 2008Applicant: Applera CorporationInventors: Timothy G. Geiser, H. Pin Kao
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Patent number: 7417726Abstract: An apparatus for normalizing a PCR instrument, can comprise a microplate comprising at least 6,000 wells and a system of dyes at known concentrations in a plurality of the at least 6,000 wells. A method for normalizing a system can comprise (a) providing a microplate comprising at least 6,000 wells and a system of dyes; (b) exciting at least one dye; (c) detecting an emission output for the at least one dye; (d) determining if the emission output is in an acceptable range; and (e) adjusting the system so that the emission output is in the acceptable range.Type: GrantFiled: March 22, 2005Date of Patent: August 26, 2008Assignee: Applied Biosystems Inc.Inventors: H. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg Young
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Publication number: 20050237528Abstract: A heating apparatus having a support base and a microplate having a first surface and an opposing second surface. The microplate being positioned adjacent the support base. A plurality of wells are formed in the first surface of the microplate and are each sized to receive an assay therein. A transparent window is positioned adjacent the microplate opposing the support base. A transparent heating element is formed on the transparent window and is operable to heat the transparent window. A control system operably controls the transparent heating element.Type: ApplicationFiled: March 22, 2005Publication date: October 27, 2005Inventors: Mark Oldham, H. Pin Kao