Patents by Inventor Hajime Matsuzaki

Hajime Matsuzaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods and compositions for multiplex amplification of nucleic acids
    Publication number: 20020058281
    Abstract: A method is described for predetermining ratios of primer pairs present in a single reaction vessel so as to achieve approximately equimolar yield of products. The ratios are determined as a function of the length of the amplicon and the length of other amplicons being simultaneously tested. The primers may desirably be for p53 gene sequences.
    Type: Application
    Filed: November 21, 2001
    Publication date: May 16, 2002
    Applicant: AFFYMETRIX, INC.
    Inventors: Hajime Matsuzaki, Eric A. Murphy
  • Nucleic acid analysis techniques
    Patent number: 6344316
    Abstract: The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase.
    Type: Grant
    Filed: June 25, 1997
    Date of Patent: February 5, 2002
    Assignee: Affymetrix, Inc.
    Inventors: David J. Lockhart, Mark Chee, Kevin Gunderson, Lai Chaoqiang, Lisa Wodicka, Maureen T. Cronin, Danny Lee, Huu M. Tran, Hajime Matsuzaki
  • Methods and compositions for multiplex amplification of nucleic acids
    Patent number: 6333179
    Abstract: A method is described for predetermining ratios of primer pairs present in a single reaction vessel so as to achieve approximately equimolar yield of products. The ratios are determined as a function of the length of the amplicon and the length of other amplicons being simultaneously tested. The primers may desirably be for p53 gene sequences.
    Type: Grant
    Filed: June 18, 1998
    Date of Patent: December 25, 2001
    Assignee: Affymetrix, Inc.
    Inventors: Hajime Matsuzaki, Eric A. Murphy