Abstract: The present invention relates to an improved method for the identification and optionally the characterization of interacting molecules designed to detect positive clones from the rather large numbers of false positive clones isolated by two-hybrid systems. The method of the invention relies on a novel combination of selection steps used to detect clones that express interacting molecules from false positive clones. The present invention further relates to a kit useful for carrying out the method of the invention. The present invention provides for parallel, high-throughput or automated interaction screens for the reliable identification of interacting molecules.

Abstract: The invention presents a method for examining genetic material (deoxyribonucleic acid, DNA) to detect the presence of pre-known mutations, especially single nucleotide polymorphisms (SNP), using mass spectrometry with ionization by matrix-assisted laser desorption (MALDI). The invention uses nucleoside triphosphates with modified sites for the method of primer extension in a duplicating, enzymatic reaction and at least partially removal of primers from the extension product, in combination with product neutralization by chemical treatment of the modified sites, so that the resulting DNA products can be, by using special matrix materials, preferredly ionized in an adduct-free form over other constituents in the reaction solution without any further cleaning. The method is particularly suitable for simultaneous identification of several mutations by multiplexing.

Abstract: The invention relates to a reaction vessel (1) for producing a sample, in particular a crystal, from a substance in solution or in liquid form, having several reaction chambers (6) each forming a separate gas chamber, consisting of at least one housing part, and each reaction chamber (6) has a reservoir (7) and several reaction areas (8) co-operating therewith, connected to one another and to the reservoir (7) in order to exchange gas. The reservoirs and the reaction areas co-operating with them are disposed immediately adjacent to one another in rows, distributed in a predeterminable, identical manner, these rows running parallel with one another. Each row of reservoirs (7) therefore co-operates with at least one row of reaction areas (8).

Abstract: The present invention relates to a method for the preselection of shotgun clones, e.g., cosmids, PACs, BACs, etc. of a genome of an organism, or of parts of the genome of an organism that significantly reduces the time and workload associated with the further processing of shotgun clones, for example, in sequencing projects such as the human genome project. The invention relies on a combination of steps including the transfer of shotgun clones to a carrier. e.g., nylon membrane, glass chip, etc. where the clones bind, preferably hybridize to a set of specifically selected probes, e.g., DNA oligonucleotides, PNA oligonucleotides or pools of DNA or/and PNA oligonucleotides, further antibodies, fragments or derivatives thereof which are labeled or unlabeled. Each probe of said set interacts to 1 to 99% (ideally 50%) of all shotgun clones (nucleic acid fragments) in all investigated shotgun libraries.

Abstract: The present invention relates to a method for the preselection of shotgun clones, e.g., cosmids, PACs, BACs, etc. of a genome of an organism, or of parts of the genome of an organism that significantly reduces the time and workload associated with the further processing of shotgun clones, for example, in sequencing projects such as the human genome project. The invention relies on a combination of steps including the transfer of shotgun clones to a carrier, e.g., nylon membrane, glass chip, etc. where the clones bind, preferably hybridize to a set of specifically selected probes, e.g., DNA oligonucleotides, PNA oligonucleotides or pools of DNA or/and PNA oligonucleotides, further antibodies, fragments or derivatives thereof which are labeled or unlabeled.

Abstract: A reaction kinetics measuring system which provides for the application of great variety of reaction initiating methods, as temperature or pressure or electrical field jump methods, mixing methods, flash photolysis method, and combinations of said methods, and for various optical detection methods, as absorption or fluorescence or scattered light or polarized light detection methods. The system features quickly and easily interchangeable optical elements in a number of optical paths, and a multitude of selectively usable measuring cells to adapt the system to the reaction initiating and detection methods used so that reliable and exactly reproducible results are obtained, which are comparable even if obtained under widely differing conditions.