Patents by Inventor Hany Ramez Nassef
Hany Ramez Nassef has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20160129441Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.Type: ApplicationFiled: October 2, 2015Publication date: May 12, 2016Inventors: Lincoln McBride, Geoffrey Facer, Marc Unger, Michael Lucero, Hany Ramez Nassef
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Patent number: 9150913Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.Type: GrantFiled: July 12, 2012Date of Patent: October 6, 2015Assignee: Fluidigm CorporationInventors: Lincoln McBride, Geoffrey Facer, Marc Unger, Michael Lucero, Hany Ramez Nassef
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Patent number: 9103825Abstract: A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.Type: GrantFiled: April 21, 2010Date of Patent: August 11, 2015Assignee: FLUIDIGM CORPORATIONInventors: Hany Ramez Nassef, Hou-Pu Chou, Michael Lucero, Andrew May, Kathy Yokobata
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Patent number: 9063026Abstract: The present teachings provide apparatus and methods for measuring the power output of a person, such as a cyclist. According to various embodiments, while measuring the power output, the present teachings provide for the harvesting of the input energy to provide electrical power to the apparatus. Among others, embodiments are described wherein power measurements are obtained from the drive train of a bicycle by making use of piezoelectric element(s) capable of producing electricity when deformed by applied forces. In addition, telemetry information of the applied forces can be obtained with the use of an inertial measurement unit.Type: GrantFiled: January 9, 2014Date of Patent: June 23, 2015Inventor: Hany Ramez Nassef
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Patent number: 8828663Abstract: Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.Type: GrantFiled: March 20, 2006Date of Patent: September 9, 2014Assignees: Fluidigm Corporation, The Regents of the University of CaliforniaInventors: Marc Unger, Lincoln McBride, Geoffrey Facer, Michael Lucero, Hany Ramez Nassef
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Publication number: 20140195103Abstract: The present teachings provide apparatus and methods for measuring the power output of a person, such as a cyclist. According to various embodiments, while measuring the power output, the present teachings provide for the harvesting of the input energy to provide electrical power to the apparatus. Among others, embodiments are described wherein power measurements are obtained from the drive train of a bicycle by making use of piezoelectric element(s) capable of producing electricity when deformed by applied forces. In addition, telemetry information of the applied forces can be obtained with the use of an inertial measurement unit.Type: ApplicationFiled: January 9, 2014Publication date: July 10, 2014Inventor: Hany Ramez Nassef
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Publication number: 20130171633Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.Type: ApplicationFiled: July 12, 2012Publication date: July 4, 2013Applicant: Fluidigm CorporationInventors: Lincoln McBride, Geoffrey Facer, Marc Unger, Michael Lucero, Hany Ramez Nassef
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Publication number: 20130089874Abstract: The present invention provides a variety of microfluidic devices and methods for conducting assays and syntheses. The devices include a solid substrate layer having a surface that is capable of attaching ligand and or anti-ligand, and an elastomeric layer attached to said surface. Preferred embodiments have deflectable membrane valves and pumps, for example, rotary pumps associated therewith.Type: ApplicationFiled: March 29, 2012Publication date: April 11, 2013Applicant: Fluidigm CorporationInventors: Ian David Manger, Joseph W. Barco, Hany Ramez Nassef
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Patent number: 8343442Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.Type: GrantFiled: August 18, 2010Date of Patent: January 1, 2013Assignee: Fluidigm CorporationInventors: Lincoln McBride, Michael Lucero, Marc Unger, Hany Ramez Nassef, Geoffrey Facer
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Patent number: 8247178Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.Type: GrantFiled: October 14, 2009Date of Patent: August 21, 2012Assignee: Fluidigm CorporationInventors: Lincoln McBride, Geoffrey Facer, Marc Unger, Michael Lucero, Hany Ramez Nassef
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Patent number: 8168139Abstract: The present invention provides a variety of microfluidic devices and methods for conducting assays and syntheses. The devices include a solid substrate layer having a surface that is capable of attaching ligand and or anti-ligand, and an elastomeric layer attached to said surface. Preferred embodiments have deflectable membrane valves and pumps, for example, rotary pumps associated therewith.Type: GrantFiled: June 23, 2003Date of Patent: May 1, 2012Assignee: Fluidigm CorporationInventors: Ian David Manger, Joseph W. Barco, Hany Ramez Nassef
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Publication number: 20110189678Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.Type: ApplicationFiled: August 18, 2010Publication date: August 4, 2011Applicant: Fluidigm CorporationInventors: Lincoln McBride, Michael Lucero, Marc Unger, Hany Ramez Nassef, Geoffrey Facer
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Publication number: 20110020918Abstract: A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.Type: ApplicationFiled: April 21, 2010Publication date: January 27, 2011Applicant: Fluidigm CorporationInventors: Hany Ramez Nassef, Hou-Pu Chou, Michael Lucero, Andrew May, Kathy Yokobata
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Patent number: 7837946Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.Type: GrantFiled: November 6, 2008Date of Patent: November 23, 2010Assignee: Fluidigm CorporationInventors: Lincoln McBride, Michael Lucero, Marc Unger, Hany Ramez Nassef, Geoffrey Facer
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Publication number: 20100184202Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.Type: ApplicationFiled: October 14, 2009Publication date: July 22, 2010Applicant: Fluidigm CorporationInventors: Lincoln McBride, Geoffrey Facer, Marc Unger, Michael Lucero, Hany Ramez Nassef
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Patent number: 7749737Abstract: An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.Type: GrantFiled: October 30, 2007Date of Patent: July 6, 2010Assignee: Fluidigm CorporationInventors: Lincoln McBride, Geoffrey Facer, Marc Unger, Michael Lucero, Hany Ramez Nassef
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Patent number: 7691333Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.Type: GrantFiled: April 5, 2004Date of Patent: April 6, 2010Assignee: Fluidigm CorporationInventors: Lincoln McBride, Michael Lucero, Marc Unger, Hany Ramez Nassef, Geoffrey Facer, Yong Yi
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Patent number: 7666361Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.Type: GrantFiled: April 5, 2004Date of Patent: February 23, 2010Assignee: Fluidigm CorporationInventors: Lincoln McBride, Marc Unger, Michael Lucero, Hany Ramez Nassef, Geoff Facer, Yong Yi
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Patent number: 7604965Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.Type: GrantFiled: March 18, 2005Date of Patent: October 20, 2009Assignee: Fluidigm CorporationInventors: Lincoln McBride, Geoffrey Facer, Marc Unger, Michael Lucero, Hany Ramez Nassef
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Publication number: 20090187009Abstract: The present invention provides methods of and systems for translating conditions from a small-volume experiment to a larger-volume experiment.Type: ApplicationFiled: October 24, 2008Publication date: July 23, 2009Applicant: Fluidigm CorporationInventors: Andrew May, Hany Ramez Nassef