Abstract: An apparatus for a lateral flow nucleic acid assay with an integrated pore-based detector, which has the potential to detect pathogens, both microbial and viral, in aqueous samples in approximately 5 minutes or less without nucleic acid amplification or optical components. The detector is based on an electromechanical signal transduction mechanism that enables low-cost detection of DNA/RNA at ultralow concentrations (down to about 10 M to about 19 M). The scheme relies on the use of charge-neutral peptide nucleic acid (PNA) capture probe conjugated to polystyrene beads. The PNA-beads acquire substantial negative charge upon capture of target pathogenic DNA/RNA and become mobile in an electric field. Upon application of a bias voltage of around 1 V to 2 V, the PNA-beads with hybridized target are directed electrophoretically to a smaller diameter pore. Subsequent pore blockage results in a strong, sustained drop in measured ionic current through the pore.

Abstract: Systems and methods for specific nucleic acid (NA) sequence detection that do not rely on polymerase chain reaction (PCR) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.

Abstract: Systems and methods for specific nucleic acid (NA) sequence detection that do not rely on polymerase chain reaction (PCR) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.

Abstract: Systems and methods for specific nucleic acid (NA) sequence detection that do not rely on polymerase chain reaction (PCR) for target sequence amplification and do not require any special reagents other than a complementary sequence capture probe conjugated to spherical beads.

Abstract: A method for producing, and a product having, a surface nanopattern, wherein the method comprises the steps of: obtaining a substrate with a smooth surface; acquiring a self-assembling monolayer precursor, wherein the precursor includes an inducible, usually photocatalytically, active region and a substrate attachment region; mixing a plurality of the self-assembling monolayer precursors with the substrate to produce a self-assembled monolayer having an exposed surface comprising the inducible active regions and anchored to the substrate smooth surface by the substrate attachment regions; obtaining a path-directable nanoparticle; contacting the path-directable nanoparticle with the exposed surface at an interface area; exposing the exposed surface contacted with the path-directable nanoparticle to an inducing event, usually exposure to light, thereby chemically altering the inducible active regions and producing a detectable state in the interface area on the exposed surface; and applying a force of variable

Abstract: A method for producing, and a product having, a surface nanopattern, wherein the method comprises the steps of: obtaining a substrate with a smooth surface; acquiring a self-assembling monolayer precursor, wherein the precursor includes an inducible, usually photocatalytically, active region and a substrate attachment region; mixing a plurality of the self-assembling monolayer precursors with the substrate to produce a self-assembled monolayer having an exposed surface comprising the inducible active regions and anchored to the substrate smooth surface by the substrate attachment regions; obtaining a path-directable nanoparticle; contacting the path-directable nanoparticle with the exposed surface at an interface area; exposing the exposed surface contacted with the path-directable nanoparticle to an inducing event, usually exposure to light, thereby chemically altering the inducible active regions and producing a detectable state in the interface area on the exposed surface; and applying a force of variable

Abstract: The invention includes various prototypical amperometric biosensors for the quantification of biological substrates such as fructose, creatinine, creatine, and sarcosine, and the methods for producing these biosensors. Also included in the invention is a minaturized version of the biosensing devices. The components of these prototypical biosensors are immobilized on a self-assembled monolayer (SAM) comprising chemisorbed alkanethiols. The deposition of an amphiphilic lipid layer to these systems increases the stability and activity of the resultant biosensor and enhances the rejection of many interferents. An additional feature of the invention is the co-deposition of the components of the sensor via a novel detergent dialysis protocol. The invention features two particular biosensor systems. One embodiment involves fructose dehydrogenase as the redox/sensor enzyme and fructose as the substrate/analyte.