Abstract: The invention relates to a novel method for evaluating the antiproliferative activity of protein deacetylase inhibiting compounds and microtubule interacting agents, as well as a method for screening for compounds that inhibit cell growth or growth of tumors. The invention additionally provides a method for monitoring the progress of treatment against cellular growth or the growth of tumors.

Abstract: The invention relates to a novel method for evaluating the antiproliferative activity of compounds having MetAP inhibitory activity, as well as a method for screening compounds that inhibit angiogenesis or growth of tumors. The invention additionally provides a method for monitoring the progress of treatment for controlling angiogenesis or the growth of tumors.

Abstract: A method of inhibiting proteolytic degradation of a thermally-stable intracellular protein is described. The method involves adding 1 or more denaturing agents to a sample which contains the protease and the protein of interest and heating the resulting solution at a temperature and for period of time sufficient to denature the protease. The method optionally includes a step for lysing the cell if the protein of interest is contained in a cell in order to release said protein. Additionally, a method of determining Mx protein induced by interferon in a blood sample is described. The method involves adding to a blood sample a lysing agent, a denaturing agent, and a detergent selected to solubilize Mx protein. The sample containing Mx protein is then heated at a temperature of from about 50.degree. C. to about 60.degree. C. for a period of time of from about 1 minute to about 30 minutes, and the Mx protein in the solution then is determined.

Abstract: New devices and kits for solid-phase immuno-assays comprising a solid porous support, preferably in the form of a sheet, where antigens or immuno-globulins or both of them are bound by direct application in any suitable geometry, e.g. as an assay of dots or lines. Such porous supports are suitable for effecting an unlimited number of antibody-antigen reactions simultaneously and in one operation.

Abstract: Solid supports for proteins consisting of nitrocellulose sheets containing a replica of an electropherogram of proteins as obtained by electrophoretic separation in a gel. Faithful replica of such electropherograms on the nitrocellulose support can be obtained by contacting the gel with a nitrocellulose sheet and applying an electric field perpendicular to the plane of the gel causing an electrophoretic migration of the proteins toward the nitrocellulose sheet where the proteins are adsorbed. Various analytical problems and especially immuno-assays, for enzyme immuno-assays or such involving radioactively labeled indicators, can be made with the new solid supports.