Patents by Inventor Heinrich Spiecker
Heinrich Spiecker has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230296515Abstract: Methods for single molecule localization microscopy may use a patterned illumination over the field of view. The patterned illumination may be dynamically adapted to the approximate positions of the detected fluorescent molecule emitters, allowing for increased signal-to-background for their single molecule localization.Type: ApplicationFiled: February 21, 2023Publication date: September 21, 2023Applicant: LaVision BioTec GmbHInventors: Heinrich Spiecker, Stefan Miltenyi, Robin DIEKMANN, Ali Kinkhabwala, Anthony deVries
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Publication number: 20230069991Abstract: The invention is directed to a process for extracting target cells from a three dimensional biological specimen by the steps imaging the three dimensional specimen; identifying target cells; registering the spatial parameters (x,y,z coordinates) of the target cells; and extraction of target cells according to their spatial parametersType: ApplicationFiled: February 25, 2021Publication date: March 9, 2023Inventors: Andreas BOSIO, Heinrich SPIECKER
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Publication number: 20220411862Abstract: The invention is directed to a method to obtain the spatial location and sequence information of at least a part of a RNA or cDNA strand (006) in a sample comprising the steps a. hybridizing a first detection probe oligonucleotide (204) comprising 50-1000 nucleotides with its 3? and/or 5? end to the complementary part of the at least one RNA or cDNA strand, wherein the detection probe oligonucleotide is partially hybridized to a bridge oligonucleotide (205) comprising 5-100 nucleotides wherein a gap region (206) capable of binding oligonucleotides is created b. filling the gap region (206) in part with 1 to 16 barcode oligonucleotides comprising 4-20 nucleotides, wherein the barcode oligonucleotides determine the spatial information of the RNA or cDNA strand in the sample c.Type: ApplicationFiled: May 24, 2022Publication date: December 29, 2022Applicant: Miltenyi Biotec B.V. & Co. KGInventors: Heinrich SPIECKER, Sandra HALBFELD, Thomas ROTHMANN, Andreas BOSIO
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Publication number: 20220243260Abstract: Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ sequencing and in vitro sequencing of rolonies derived from rolling circle amplification from padlock oligonucleotides targeting portion of RNA or cDNA transcript at a subcellular level with less limitation in the amount of transcripts and the length of the sequence that can be analyzed. Apart from padlocks oligonucleotides, the SUMI-Seq method can also be applied using circular oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.Type: ApplicationFiled: January 31, 2022Publication date: August 4, 2022Applicant: Miltenyi Biotec B.V. & Co. KGInventors: Thomas ROTHMANN, Robert PINARD, Hansueli MEYER, Andreas BOSIO, Heinrich SPIECKER, Seiyu HOSONO, Ryan HINDMAN, Emily NEIL, Chris NEHME
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Publication number: 20220026696Abstract: The present application discloses a light sheet microscope for imaging biological materials. The microscope uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.Type: ApplicationFiled: December 10, 2019Publication date: January 27, 2022Applicant: LaVision BioTec GmbHInventors: Heinrich Spiecker, Andreas HEYMANN
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Publication number: 20220003979Abstract: The present application discloses a light sheet microscope for imaging biological materials. The microscope uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.Type: ApplicationFiled: December 10, 2019Publication date: January 6, 2022Applicant: LaVision BioTec GmbHInventors: Heinrich Spiecker, Martin Schuette
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Publication number: 20210373309Abstract: The present application discloses a light sheet microscope for imaging biological materials. The microscope uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.Type: ApplicationFiled: December 10, 2019Publication date: December 2, 2021Applicant: LaVision BioTec GmbHInventor: Heinrich Spiecker
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Patent number: 10126230Abstract: The invention relates to a device for examining samples (1) in a liquid (5), comprising a movable shaft (2), to which the sample (1) is fastened, and a cuvette (4), wherein the device further comprises a bath (3), which surrounds the movable shaft (2), and wherein the bath (3) is fillable with the liquid (5), the movable shaft (2) is configured to receive the sample (1) at the upper side (24) thereof, the movable shaft (2) reaches into the cuvette (4) from below, wherein said cuvette is open at least toward the bottom and configured to be immersed into the liquid (5) in the bath (3) with the underside thereof, and, moreover, means are provided to generate a pressure difference between the interior of the cuvette (4) and the region outside of the cuvette (4) such that the fill level (21) of the liquid (5) in the cuvette (4) is adjustable. Moreover, the invention relates to a method for examining samples (1) in a liquid (5).Type: GrantFiled: June 3, 2014Date of Patent: November 13, 2018Assignee: LAVISION BIOTEC GMBHInventors: Raoul Amadeus Lorbeer, Marko Heidrich, Heiko Meyer, Heinrich Spiecker
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Patent number: 9705275Abstract: The invention relates to a laser assembly (100) having a laser (L) for generating primary laser pulses (1), beam splitting optics (15) for splitting a primary laser pulse into a plurality of temporally staggered sub-pulses, and having focusing optics (17-19) for focusing the sub-pulses in or on an object (20) so that every sub-pulse is focused in a separate focus volume (F). The invention is characterized in that the mutual spatial and/or temporal relationship of the focus volumes (F) of the sub-pulses originating from a common primary laser pulse is variably adjustable. The invention also relates to a corresponding method.Type: GrantFiled: August 10, 2012Date of Patent: July 11, 2017Assignee: LAVISION BIOTEC GMBHInventor: Heinrich Spiecker
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Publication number: 20160109357Abstract: The invention relates to a device for examining samples (1) in a liquid (5), comprising a movable shaft (2), to which the sample (1) is fastened, and a cuvette (4), wherein the device further comprises a bath (3), which surrounds the movable shaft (2), and wherein the bath (3) is fillable with the liquid (5), the movable shaft (2) is configured to receive the sample (1) at the upper side (24) thereof, the movable shaft (2) reaches into the cuvette (4) from below, wherein said cuvette is open at least toward the bottom and configured to be immersed into the liquid (5) in the bath (3) with the underside thereof, and, moreover, means are provided to generate a pressure difference between the interior of the cuvette (4) and the region outside of the cuvette (4) such that the fill level (21) of the liquid (5) in the cuvette (4) is adjustable. Moreover, the invention relates to a method for examining samples (1) in a liquid (5).Type: ApplicationFiled: June 3, 2014Publication date: April 21, 2016Applicants: Laser Zentrum Hannover e.V., La Vision BioTec GmbHInventors: Raoul Amadeus LORBEER, Marko HEINDRICH, Heiko Meyer, Heinrich SPIECKER
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Publication number: 20140246612Abstract: The invention relates to a laser assembly (100) having a laser (L) for generating primary laser pulses (1), beam splitting optics (15) for splitting a primary laser pulse into a plurality of temporally staggered sub-pulses, and having focusing optics (17-19) for focusing the sub-pulses in or on an object (20) so that every sub-pulse is focused in a separate focus volume (F). The invention is characterized in that the mutual spatial and/or temporal relationship of the focus volumes (F) of the sub-pulses originating from a common primary laser pulse is variably adjustable. The invention also relates to a corresponding method.Type: ApplicationFiled: August 10, 2012Publication date: September 4, 2014Applicant: LAVISION BIOTEC GmbHInventor: Heinrich Spiecker
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Patent number: 7211807Abstract: The subject matter of the invention is a readout method performed by stripe scanning planar objects with substances emitting fluorescence radiation, a detector being provided and said fluorescence radiation being imaged onto said detector, said detector comprising at least one row of N-linearly disposed single detector segments, with the scanning direction being rotated by angle ? with respect to the at least one detector row.Type: GrantFiled: March 25, 2005Date of Patent: May 1, 2007Assignee: LaVision BioTec GmbHInventor: Heinrich Spiecker
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Patent number: 7088517Abstract: The subject matter of the invention is a beam splitter device having two spaced apart parallel highly reflecting mirrors, between which there is disposed a semireflecting mirror which is spaced a different distance from the at least one highly reflecting mirror, said semireflecting mirror having at least one portion configured to form a full reflector or an antireflector, the semireflecting mirror being slidably disposed between the highly reflecting mirrors and a laser-scanning microscope as well.Type: GrantFiled: March 19, 2003Date of Patent: August 8, 2006Assignee: LaVision Biotec GmbHInventor: Heinrich Spiecker
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Publication number: 20050211913Abstract: The subject matter of the invention is a readout method performed by stripe scanning planar objects with substances emitting fluorescence radiation, a detector being provided and said fluorescence radiation being imaged onto said detector, said detector comprising at least one row of N-linearly disposed single detector segments, with the scanning direction being rotated by angle ? with respect to the at least one detector row.Type: ApplicationFiled: March 25, 2005Publication date: September 29, 2005Inventor: Heinrich Spiecker
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Patent number: 6737647Abstract: An array for achromatic imaging of a pulsed beam of charged particles is described. The device comprises an imaging system with a round lens optics (2, 7), a low-energy drift space (3) and an accelerator (4-6) driven by at least one rapidly switchable voltage U(t), such that the velocity and/or trajectory of the particles in the beam is influenced and the particles starting from point (1) with different energies are crossing the image plane in one point (8), i.e. the chromatic aberration of the optics is compensated.Type: GrantFiled: April 17, 2003Date of Patent: May 18, 2004Inventors: Gerd Schönhense, Heinrich Spiecker
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Publication number: 20040000646Abstract: An array for achromatic imaging of a pulsed ensemble of charged particles is described. The device comprises an imaging system with a round lens optics (2, 7), a low-energy drift space (3) and an accelerator (4-6) driven by at least one rapidly switchable voltage U(t), such that the velocity and/or trajectory of the particles in the ensemble is influenced and the particles starting from point (1) with different energies are crossing the image plane in one point (8), i.e. the chromatic aberration of the optics is compensated.Type: ApplicationFiled: April 17, 2003Publication date: January 1, 2004Inventors: Gerd Schonhense, Heinrich Spiecker
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Publication number: 20030235905Abstract: A base member for a biochip, e.g., a chip for analyzing DNA, RNA, proteins or cells, the base member (1), which is shaped like a board, being provided with a plurality of discrete elevations (2) extending from the upper side (1a) to the underside and having, in the region of their lower end, an active surface (3) for receiving the probe material.Type: ApplicationFiled: June 12, 2003Publication date: December 25, 2003Applicant: LaVision BioTec GmbHInventor: Heinrich Spiecker
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Publication number: 20030223115Abstract: The subject matter of the invention is a beam splitter device comprising at least two spaced apart highly reflective mirrors between the two of which a partially reflecting mirror is disposed, said partially reflecting mirror having a partial reflectivity coating and the coating being formed in such a manner that the reflection curve produced by the coating exceeds the reflection coefficient of 50% in the range of at least one wavelength.Type: ApplicationFiled: May 21, 2003Publication date: December 4, 2003Inventor: Heinrich Spiecker
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Publication number: 20030189757Abstract: The subject matter of the invention is a beam splitter device having two spaced apart parallel highly reflecting mirrors, between which there is disposed a semireflecting mirror which is spaced a different distance from the at least one highly reflecting mirror, said semireflecting mirror having at least one portion configured to form a full reflector or an antireflector, the semireflecting mirror being slidably disposed between the highly reflecting mirrors and a laser-scanning microscope as well.Type: ApplicationFiled: March 19, 2003Publication date: October 9, 2003Applicant: LaVision BioTec GmbHInventor: Heinrich Spiecker