Abstract: The invention provides methods of engineering plants having lignin deposition or xylan deposition that is substantially localized to the vessels of xylem tissue in the plant. The invention also provides methods of engineering plants to increase production of a desired biosynthetic product, e.g., to have increased secondary cell wall deposition or increased wax/cutin accumulation. The engineered plants of the present invention have use in bioenergy production, e.g., by improving the density and the digestibility of biomass derived from the plant and to improve water usage requirements.

Abstract: The present invention provides for a polypeptide capable of dominant suppression of a first naturally occurring IRX10, wherein the polypeptide comprises an amino acid sequence having at least 70% identity as compared to a second naturally occurring IRX10 wherein the polypeptide comprises one or more of the conserved amino acid indicated in FIG. 2 substituted with a different amino acid residue.

Abstract: The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Abstract: The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Abstract: The invention provides methods for engineering plants to have reduced levels of acetylation by decreasing expression of one or more Cas1L genes. Such plants can be used, e.g., to increase yield for biofuel production.

Abstract: The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Abstract: The present invention provides for a method of engineering a plant having an increased content of a target compound in the plant's seed, the method comprising introducing into the plant a first nucleic acid construct that encodes a seed-specific promoter operatively linked to a transcription factor wherein expression of the transcription factor increases the production of the target compound. A genetically modified plant cell engineered by the method.

Abstract: The invention provides compositions and methods for inhibiting the expression of the gene XAX1 in grass plants. Plants with inhibited expression of XAX1 have use, e.g., in biofuel production by increasing the amount of soluble sugar that can be extracted from the plant.

Abstract: The present invention provides for dominant negative mutations of Arabidopsis REDUCING WALL ACETYLATION (RWA).

Abstract: The invention provides methods of engineering plants having lignin deposition or xylan deposition that is substantially localized to the vessels of xylem tissue in the plant. The invention also provides methods of engineering plants to increase production of a desired biosynthetic product, e.g., to have increased secondary cell wall deposition or increased wax/cutin accumulation. The engineered plants of the present invention have use in bioenergy production, e.g., by improving the density and the digestibility of biomass derived from the plant and to improve water usage requirements.

Abstract: The invention provides methods of engineering latex-producing plants, e.g., guayule plants, to increase latex production. The invention additionally provides plants engineered in accordance with the invention and methods of using the plants to produce latex.

Abstract: The present invention provides for a polypeptide capable of dominant suppression of a first naturally occurring IRX10, wherein the polypeptide comprises an amino acid sequence having at least 70% identity as compared to a second naturally occurring IRX10 wherein the polypeptide comprises one or more of the conserved amino acid indicated in FIG. 2 substituted with a different amino acid residue.

Abstract: The invention provides methods of engineering guayule plants to increase latex production. Specifically, the invention provides a method of engineering guayule plants with gene constructs that allow expression of master regulators of stress responses under non-stress conditions, the method comprising: introducing an expression cassette into the guayule plant, wherein the expression cassette comprises a polynucleotide encoding a dehydration response element binding protein/C-repeat binding factors (DREB/CBF) or DEAR [DREB and C-terminal EAR (ethylene response factor associated amphiphilic repression) motif] transcription factor operably linked to a promoter, such as a stem-specific promoter or an alcohol-inducible promoter. The invention additionally provides plants engineered in accordance with the invention and methods of using the plants to produce latex.

Abstract: The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Abstract: The disclosure provides methods of engineering plants to modulate galactan content. Specifically, the disclosure provides methods for engineering a plant to increase the galactan content in a plant tissue by inducing expression of beta-1,4-galactan synthase (GALS), modulated by a heterologous promoter. Further disclosed are the methods of modulating expression level of GALS under the regulation of a transcription factor, as well as overexpression of UDP-galactose epimerse in the same plant tissue. Tissue specific promoters and transcription factors can be used in the methods are also provided.

Abstract: The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Abstract: The invention provides methods for engineering plants to have reduced levels of acetylation by decreasing expression of one or more Cas1L genes. Such plants can be used, e.g., to increase yield for biofuel production.

Abstract: The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Abstract: The disclosure provides methods of engineering plants to modulate galactan content. Specifically, the disclosure provides methods for engineering a plant to increase the galactan content in a plant tissue by inducing expression of beta-1,4-galactan synthase (GALS), modulated by a heterologous promoter. Further disclosed are the methods of modulating expression level of GALS under the regulation of a transcription factor, as well as overexpression of UDP-galactose epimerse in the same plant tissue. Tissue specific promoters and transcription factors can be used in the methods are also provided.

Abstract: The present invention provides for a genetically modified eukaryotic host cell comprising (a) a gene encoding a first nucleotide sugar transporter (NST) operably linked to a promoter, wherein the gene and/or the promoter is heterologous to the cell, and/or (b) a native gene encoding a second NTS is disrupted and/or a promoter of the native gene is disrupted. Such modified cells can be altered in the production of polysaccharide and/or glycopeptides. The present invention also provides for methods of making or using such modified cells.