Abstract: A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.

Abstract: A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.

Abstract: Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.

Abstract: Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction.

Abstract: A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.

Abstract: Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.

Abstract: A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.

Abstract: A method of polishing a fired ceramic article such as brick, tile etc article comprising applying to a surface of fired ceramic article a hardening/densifying compound comprising a member selected from the group consisting of hydroxides, silicates, siliconates, fluorosilicates, siloxanes, silazanes, silanes, silicon esters, and combinations thereof and allowing the hardening/densifying compound to contact with the surface for a period of time sufficient to allow the compound to harden surface, which can then be ground, polished etc to a high gloss.

Abstract: A method of polishing a fired ceramic article such as brick, tile etc article comprising applying to a surface of fired ceramic article a hardening/densifying compound comprising a member selected from the group consisting of hydroxides, silicates, siliconates, fluorosilicates, siloxanes, silazanes, silanes, silicon esters, and combinations thereof and allowing the hardening/densifying compound to contact with the surface for a period of time sufficient to allow the compound to harden surface, which can then be ground, polished etc to a high gloss.

Abstract: A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.

Abstract: Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction.

Abstract: A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.

Abstract: A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.

Abstract: A method and laser cutting system for cutting a secondary piece from a board is disclosed. A board is provided having a generally planar first and second surfaces separated by a predetermined distance or thickness and having a central plane located midway between the first and second surfaces. First and second lenses are arranged relative to the board with the focal point of the respective first and second lenses disposed intermediate the first and second surfaces of the board. Laser beams from a pair of lasers are propagated through the respective lenses with each laser beam converging at the focal point of its respective lens and cutting the workpiece from its respective surface toward the other surface. Ideally, the focused beams diverge sufficiently along the cutting path such that each laser beam reduces in intensity sufficiently so as not to cause harm to the opposing laser delivering systems or support structure.

Abstract: This invention relates to a connector for multi-unit coaxial cable of the type wherein each center conductor has a individual shield and each shield has a separate drain wire. More particularly, the connector includes a plurality of contact members each of which consists of an opposing cantilever contact spring section for mating with contact elements of other electrical circuits, a dual in-line cantilever, torsion bar terminating section for receiving either the coaxial cable center conductor or the drain wire, a housing to hold the plurality of contact members and a strain relief clamp member.

Abstract: This invention relates to a connector for multi-unit coaxial cable of the type wherein each center conductor has an individual shield and each shield has a separate drain wire. More particularly, the connector includes a plurality of contact members each of which consists of an opposing cantilever contact spring section for mating with contact elements of other electrical circuits, a dual in-line cantilever, torsion bar terminating section for receiving either the coaxial cable center conductor or the drain wire, a housing to hold the plurality of contact members and a strain relief clamp member.

Abstract: This invention relates to a device which removably latches a ribbon coaxial cable connector to a printed circuit board. More particularly the device has a pair of depending legs each having a pair of oppositely projecting latches. One set of latches adapted to removably retain the connector to the printed circuit board and the second set of latches adapted to provide a removable cover for the connector.