Abstract: A culturing system and method particularly useful for producing cellular products such as viral pathogens of cells. It includes a mass transfer culture segment, stacked filter plates to adjust the medium composition, and a product removal and concentration segment. The mass transfer culture segment utilizes changed directional flow of the medium to maximize cell growth and production of product. The stacked filter plates allow addition of sterile fresh medium and removal of growth inhibitory substances.

Abstract: A process and apparatus for purifying one or more target substances from a source liquid, employing one or more cross-flow filter elements, and one or more types of chromatography resins, in combination, to provide purification with advantageous yield, product purity, and cost- and time-efficiency.

Abstract: A process and apparatus for purifying one or more target substances from a source liquid, employing one or more cross-flow filter elements, and one or more types of chromatography resins, in combination, to provide purification with advantageous yield, product purity, and cost -and time-efficiency.

Abstract: A culturing system and method particularly useful for producing cellular products such as viral pathogens of cells. It includes a mass transfer culture segment, stacked filter plates to adjust the medium composition, and a product removal and concentration segment. The mass transfer culture segment utilizes changed directional flow of the medium to maximize cell growth and production of product. The stacked filter plates allow addition of sterile fresh medium and removal of growth inhibitory substances.

Abstract: A mass transfer apparatus and method is based upon use of a mass transfer chamber, such as a hollow fiber membrane bioreactor, with a control to change the directional flow therethrough for enhancement of the mass transfer operation. The invention has particular utility in cell growth systems, where the periodic reversal of flow direction of a nutrient stream passed through the bioreactor equalizes dispersion of cell growth through the bioreactor and enhances the transport of metabolic waste from the cells being grown.

Abstract: A culturing system and method particularly useful for producing cellular products such as viral pathogens of cells. It includes a mass transfer culture segment, stacked filter plates to adjust the medium composition, and a product removal and concentration segment. The mass transfer culture segment utilizes changed directional flow of the medium to maximize cell growth and production of product. The stacked filter plates allow addition of sterile fresh medium and removal of growth inhibitory substances.

Abstract: A multi-arm spider fitting including a central hub from which such arms radiate. The arms are adapted at their extremities for coupling with filter modules, so that multiple filter modules can be secured to a single spider fitting, with the filter modules being arranged in a compact array to minimize the volumetric and spatial requirements of the filtration system. The multi-module filter assembly may be mounted on a motive cart, to provide for ready transport of the filter assembly between locations in a process plant.

Abstract: A filtration cassette of a type comprising filter sheets arranged in a multilaminate, peripherally bonded array, wherein the filter sheets alternate with permeate and retentate sheets. A stacked assembly of such filtration cassettes may be employed to form a stacked cassette filter. The filtration cassettes and filter of the invention may be advantageously employed for dewatering of aqueous biomass suspensions, desalting of proteins, removal of secreted metabolites from cellular suspensions, and the like.

Abstract: A filtration cassette, of a type comprising membrane filter sheets arranged in a peripherally bonded array of multilaminate character wherein the filter sheets alternate with foraminous (e.g., screen or mesh) sheet members, ribbed retentate sheet members, and a cross-flow filter comprising a multiplicity of stacked filtration cassettes of such type. The filtration cassettes and filter of the invention may be advantageously employed for dewatering of aqueous biomass suspensions, desalting of proteins, removal of secreted metabolites from cellular suspensions, and the like.

Abstract: A filtration cassette, of a type comprising membrane filter sheets arranged in a peripherally bonded array of multilaminate character wherein the filter sheets alternate with foraminous (e.g., screen or mesh) sheet elements, and a cross-flow filter comprising a multiplicity of stacked filtration cassettes of such type. The filtration cassettes and filter of the invention may be advantageously employed for dewatering of aqueous biomass suspensions, desalting of proteins, removal of secreted metabolites from cellular suspensions, and the like.

Abstract: The present invention relates to a method of purifying native, intact fibrinogen from a liquid sample containing contaminants having molecular weights higher and/or lower than that of the fibrinogen. The method comprises subjecting the sample to filtration using one or more filters having a molecular weight cut-off such that the native, intact fibrinogen is separated from the contaminants.

Abstract: A filter plate having a flow channel area on its main top surface, and liquid distribution and collection basins, each of polygonal shape, on its main bottom surface. Liquid inlet and discharge trough openings extend through the plate adjacent the respective distribution and collection basins, to commnicate the basins on the main bottom surface of the plate with the flow channel area on the main top surface thereof. Such plates may be associated in opposedly facing pairs to form enclosed flow channels characterized by fluid flow patterns which are substantially uniform across the full transverse extent of the flow path and which facilitate the utilization of the full areal extent of the filter media employed therewith. Also described is a unitary filter element support which may be usefully employed in the filter.