Abstract: A layer stack including a first bonding dielectric material layer, a dielectric metal oxide layer, and a second bonding dielectric material layer is formed over a top surface of a substrate including a substrate semiconductor layer. A conductive material layer is formed by depositing a conductive material over the second bonding dielectric material layer. The substrate semiconductor layer is thinned by removing portions of the substrate semiconductor layer that are distal from the layer stack, whereby a remaining portion of the substrate semiconductor layer includes a top semiconductor layer. A semiconductor device may be formed on the top semiconductor layer.

Abstract: A device and method for isolating extracellular vesicles from biofluids is disclosed. A nanoporous silicon nitride membrane is provided with a tangential flow of biofluid. A pressure gradient through the nanoporous silicon nitride membrane facilitates capture of extracellular vesicles from the tangential flow vector of biofluid. Reversal of the pressure gradient results in the release of the extracellular vesicles for subsequent collection.

Abstract: Systems and methods are disclosed for automated generation of integrated circuit designs and associated data. These allow the design of processors and SoCs by a single, non-expert who understands high-level requirements; allow the en masse exploration of the design-space through the generation processors across the design-space via simulation, or emulation; allow the easy integration of IP cores from multiple third parties into an SoC; allow for delivery of a multi-tenant service for producing processors and SoCs that are customized while also being pre-verified and delivered with a complete set of developer tools, documentation and related outputs. Some embodiments, provide direct delivery, or delivery into a cloud hosting environment, of finished integrated circuits embodying the processors and SoCs.

Abstract: A compact optical device, such as an optical transmitter or transceiver, including a folded configuration, where an optical signal generated by a laser source propagates in a first direction, then is redirected in an orthogonal direction, and then redirected again to propagate in a second direction opposite the first direction. In accordance with the folded configuration, the optical signal from the laser source is coupled to a Mach-Zehnder interferometer (MZI) modulator that includes a thin-film lithium niobate (TFLN) waveguide coupled to a radio frequency (RF) transmission line to produce an RF signal modulated optical signal for remote transmission.

Abstract: The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.

Abstract: A device and method for isolating extracellular vesicles from biofluids is disclosed. A nanoporous silicon nitride membrane is provided with a tangential flow of biofluid. A pressure gradient through the nanoporous silicon nitride membrane facilitates capture of extracellular vesicles from the tangential flow vector of biofluid. Reversal of the pressure gradient results in the release of the extracellular vesicles for subsequent collection.

Abstract: An optical signal transmitter includes a laser source configured to generate light with different wavelengths, respectively; a wavelength division (WD) demultiplexer configured to redirect the light in different directions based on the different wavelengths, respectively; and a lens array including an array of lenses configured to collimate the light from the WD demultiplexer for transmission in different directions, respectively. The optical signal transmitter may be implemented in a light detection and ranging (LIDAR) apparatus. The optical signal transmitter may further include a 1×N splitter and a set of WD demultiplexers to increase the number of distinct optical signal transmissions.

Abstract: An optical signal transmitter configured to emit wavelength-distinct optical signals along distinct directions to provide a field scanning effect without requiring moving parts to effectuate the scanning. In one implementation, the optical signal transmitter includes a laser source for generating an optical signal including a set of distinct wavelengths, an optical amplifier configured to amplify the optical signal, a wavelength splitter configured to generate a set of optical signals consisting of distinct wavelengths, respectively, and a set of emission ports for emitting the set of optical signals in different directions. In another implementation, the wavelength splitting occurs at the set of emission ports. In yet another implementation, two stages of wavelength splitting are provided. The optical signal transmitter may be implemented in a LIDAR system for ascertaining information concerning objects-of-interests.

Abstract: The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3? terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3? terminal nucleotide such that the selected nucleotide becomes a 3? terminal nucleotide, and repeating the addition step until the polynucleotide is formed.

Abstract: A fiber-based accelerometer (FAC) detector includes a base; first and second flexures mechanically coupled to the base; first and second masses mechanically coupled to the first and second flexures, respectively; and a fiber Bragg grating (FBG) fiber mechanically coupled to the first and second masses, wherein the first and second flexures and the first and second masses are configured to move in response to a force and/or acceleration acting upon the first and second masses to produce a compression or expansion of the first FBG fiber.

Abstract: An optical-based system for sensing parameters of a structure or machine for monitoring the health of the structure or machine. The optical-based system includes a set of optical fibers, each optical fiber including a set of fiber Bragg grating (FBG) sensors for sensing a set of parameters of the object; a set of interrogators configured to generate a set of incident optical signals for transmission via the set of optical fibers, respectively, receive a set of reflected optical signals from each of the optical fibers of the set, and generate a set of data related to sensed parameters of the object based on the set of reflected optical signals; and a controller configured to control the set of interrogators. Via a user interface or a remote computer on a cloud, sensing instructions may be provided to the controller, and sensed data and other information may be received from the controller.

Abstract: The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3? terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3? terminal nucleotide such that the selected nucleotide becomes a 3? terminal nucleotide, and repeating the addition step until the polynucleotide is formed.

Abstract: A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.

Abstract: The present disclosure provides methods of activating an enzyme, such as error prone or template independent polymerase, using electricity to alter pH of a reaction zone and reaction site from an inactivating pH at which the enzyme is inactive to an activating pH at which the enzyme is active to add a nucleotide to an initiator or growing polymer chain. The activating pH can then be changed back to an inactivating pH and the process repeated as many times as desired to produce a target nucleic acid sequence.

Abstract: Methods for making a polynucleotide is provided. The methods include (a) providing a first single stranded oligonucleotide, (b) providing a second single stranded oligonucleotide under conditions wherein the first single stranded oligonucleotide anneals to the second single stranded oligonucleotide thereby forming a double stranded oligonucleotide template having an extendible end comprising the 3? terminal nucleotide of the first single stranded oligonucleotide, (c) providing a reaction mixture to the double stranded initiator wherein the reaction mixture comprises an enzyme, a selected nucleotide triphosphate, and divalent cations, and wherein the enzyme extends the extendible end, (d) regenerating an extendible end of the extended template, and repeating steps (c) to (d) until a polynucleotide of a desired sequence or information content is formed, with the proviso that step (d) is not required to be performed after the polynucleotide is formed.

Abstract: The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.

Abstract: An optical signal transmitter includes a laser source configured to generate light with different wavelengths, respectively; a wavelength division (WD) demultiplexer configured to redirect the light in different directions based on the different wavelengths, respectively; and a lens array including an array of lenses configured to collimate the light from the WD demultiplexer for transmission in different directions, respectively. The optical signal transmitter may be implemented in a light detection and ranging (LIDAR) apparatus. The optical signal transmitter may further include a 1xN splitter and a set of WD demultiplexers to increase the number of distinct optical signal transmissions.

Abstract: An optical signal transmitter configured to emit wavelength-distinct optical signals along distinct directions to provide a field scanning effect without requiring moving parts to effectuate the scanning In one implementation, the optical signal transmitter includes a laser source for generating an optical signal including a set of distinct wavelengths, an optical amplifier configured to amplify the optical signal, a wavelength splitter configured to generate a set of optical signals consisting of distinct wavelengths, respectively, and a set of emission ports for emitting the set of optical signals in different directions. In another implementation, the wavelength splitting occurs at the set of emission ports. In yet another implementation, two stages of wavelength splitting are provided. The optical signal transmitter may be implemented in a LIDAR system for ascertaining information concerning objects-of-interests.

Abstract: Methods and compositions are provided for modulating expression of a target nucleic acid sequence within a non-E. coli cell. The method includes providing the cell with a guide RNA comprising a portion that is complementary to all or a portion of the target nucleic acid sequence, and providing the cell a Cas protein, wherein the guide RNA and the Cas protein co-localize at the target nucleic acid sequence and wherein the Cas protein modulate the expression of the target nucleic acid sequence.

Abstract: A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.