Patents by Inventor Heung-Chae Jung
Heung-Chae Jung has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 10908163Abstract: The present invention relates to a method for analyzing the interaction between a binding protein and a target material, including measuring the interaction between the binding protein and a target material using an interaction trapper (IT) cell. The IT cell has the binding protein displayed on the surface of intracellular inclusion bodies, i.e., insoluble aggregates, by expressing a fusion protein which forms active protein particles containing the binding protein. The method includes increasing cell permeability without affecting the activity of the binding protein displayed on the cells and genetic information.Type: GrantFiled: August 4, 2016Date of Patent: February 2, 2021Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Seung-Goo Lee, Yu Jung Kim, Jongsik Gam, Haseong Kim, Dae-Hee Lee, Heung-Chae Jung
-
Publication number: 20170192009Abstract: The present invention relates to a method for analyzing the interaction between a binding protein and a target material, including measuring the interaction between the binding protein and a target material using an interaction trapper (IT) cell. The IT cell has the binding protein displayed on the surface of intracellular inclusion bodies, i.e., insoluble aggregates, by expressing a fusion protein which forms active protein particles containing the binding protein. The method includes increasing cell permeability without affecting the activity of the binding protein displayed on the cells and genetic information.Type: ApplicationFiled: August 4, 2016Publication date: July 6, 2017Applicant: Korea Research Institute of Bioscience and BiotechnologyInventors: Seung-Goo Lee, Yu Jung Kim, Jongsik Gam, Haseong Kim, Dae-Hee Lee, Heung-Chae Jung
-
Patent number: 8669078Abstract: Disclosed herein are ammonia-specific 5?-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5?-XMP aminase. The resulting 5?-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5?-XMP into 5?-GMP. Thus, the ammonia-specific 5?-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5?-XMP into 5?-GMP.Type: GrantFiled: December 14, 2006Date of Patent: March 11, 2014Assignee: CJ Cheiljedang CorporationInventors: Jae-Gu Pan, Heung-Chae Jung, Eui-Joong Kim, Han-Seung Lee, Young Hoon Park, Hyoung Suk Kim, Jong-Kwon Han, Jin Nam Lee, Ki-Hoon Oh, Jeong Hwan Kim, Yoon-Suk Oh, Jae Ick Sim, Kuk-Ki Hong, Kyung Oh Choi, Hyun Soo Kim, Min-Ji Baek, Tae Sun Kang
-
Patent number: 8030064Abstract: The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method.Type: GrantFiled: December 30, 2003Date of Patent: October 4, 2011Assignees: Korea Advanced Institute of Science and Technology, Genofocus, Inc.Inventors: Sang Yup Lee, Tae Jung Park, Jong Pil Park, Seok Jae Lee, Jae-Gu Pan, Heung-Chae Jung, Soo-Keun Choi
-
Patent number: 7785832Abstract: The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment for a gene that encodes a desired protein, wherein said promoter has an inductive activity for transcription during the resting stage of cell growth and also the induction of said protein expression can be controlled by varying culturing conditions.Type: GrantFiled: January 20, 2006Date of Patent: August 31, 2010Assignee: HALLA Patent & Law FirmInventors: Jae-Gu Pan, Heung-Chae Jung, Sooan Shin
-
Patent number: 7582426Abstract: The present invention relates to a method for display of proteinson spore surface and a method for improving protein with rapidity using the same, which comprises the steps of (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding a protein of interest, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the protein of interest, (ii) transforming a host cell with the vector for spore surface display; (iii) displaying the protein of interest on a surface of a spore of the host cell; and (iv) recovering the spore displaying on its surface the protein of interest.Type: GrantFiled: December 7, 2001Date of Patent: September 1, 2009Assignee: Geneofocus Co., Ltd.Inventors: June-Hyung Kim, Byung-Gee Kim, Soo-Keun Choi, Heung-Chae Jung, Jae-gu Pan
-
Publication number: 20080318278Abstract: Disclosed herein are ammonia-specific 5?-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5?-XMP aminase. The resulting 5?-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5?-XMP into 5?-GMP. Thus, the ammonia-specific 5?-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5?-XMP into 5?-GMP.Type: ApplicationFiled: December 14, 2006Publication date: December 25, 2008Applicant: CJ CHEILJEDANG CORPORATIONInventors: Jae-Gu Pan, Heung-Chae Jung, Eui-Joong Kim, Han-Seung Lee, Young Hoon Park, Hyoung Suk Kim, Jong-Kwon Han, Jin Nam Lee, Ki-Hoon Oh, Jeong Hwan Kim, Yoon-Suk Oh, Jae Ick Sim, Kuk-Ki Hong, Kyung Oh Choi, Hyun Soo Kim, Min-Ji Baek, Tae Sun Kang
-
Publication number: 20070269838Abstract: The present invention relates to a method for expressing a target protein on an exosporium forming the outermost surface of bacterial spores. More particularly, the present invention relates to a method for expressing a target protein on the surface of cells and spores using an exosporium as a matrix for surface expression, and methods for the production of a protein array, the production of antibodies, the separation of a certain substance from a mixture, bioconversion, and the improvement of a target protein, which are characterized by using the cells or spores having the target protein that was expressed on the surface by the above expression method.Type: ApplicationFiled: December 30, 2003Publication date: November 22, 2007Inventors: Sang Lee, Tae Park, Jong Park, Seok Lee, Jae-Gu Pan, Heung-Chae Jung, Soo-Keun Choi
-
Publication number: 20060141578Abstract: The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment for a gene that encodes a desired protein, wherein said promoter has an inductive activity for transcription during the resting stage of cell growth and also the induction of said protein expression can be controlled by varying culturing conditions.Type: ApplicationFiled: January 20, 2006Publication date: June 29, 2006Inventors: Jae-Gu Pan, Heung-Chae Jung, Sooan Shin
-
Publication number: 20050176096Abstract: The present invention relates to a biocatalyst displayed on spore or virus surface and a method of bioconversion using the same, in particular to a method of bioconversion using a biocatalyst, which comprises the steps of: (a) preparing a vector for spore surface display comprising a gene construct containing a gene encoding a display motif and a gene encoding the biocatalyst, wherein, when expressed, the gene construct expresses the display motif and the biocatalyst in a fusion form and the biocatalyst is displayed on a spore surface; (b) transforming a host cell with the vector for spore surface display; (c) displaying the biocatalyst on the spore surface of the host cell; (d) recovering the spore displaying on its surface the biocatalyst; and (e) performing the bioconversion reaction using the spore displaying on its surface the biocatalyst, and a biocatalyst.Type: ApplicationFiled: April 9, 2002Publication date: August 11, 2005Inventors: Seok-Joon Kwon, Soo Keun Choi, Heung Chae Jung, Jae Gu Pan, Joon Sung Chang, Tae Jung Park
-
Publication number: 20040191861Abstract: The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment for a gene that encodes a desired protein, wherein said promoter has an inductive activity for transcription during the resting stage of cell growth and also the induction of said protein expression can be controlled by varying culturing conditions.Type: ApplicationFiled: June 30, 2003Publication date: September 30, 2004Applicant: Korean Research Institute of Bioscience and BiotechnologyInventors: Jae-Gu Pan, Heung-Chae Jung, Sooan Shin
-
Publication number: 20040180348Abstract: The present invention relates to methods for preparing a protein of interest surface-displayed on genetic carrier, for improving a protein of interest, for isolating a substance of interest, bioconversion and producing antibody. More particularly, the present invention relates to a method for preparing a protein of interest surface-displayed on genetic carrier, which comprises the steps of: (a) transforming a host cell harboring the genetic carrier selected from the group consisting of spore and virus with a vector containing a gene encoding the protein of interest; (b) culturing the transformed host cell and expressing the protein of interest in the host cell; and (c) allowing to form noncovalent bond between the expressed protein and a surface of the genetic carrier so that the protein of interest is displayed on the surface of the genetic carrier.Type: ApplicationFiled: April 26, 2004Publication date: September 16, 2004Inventors: Jae-Gu Pan, Soo-Keun Choi, Heung-Chae Jung
-
Publication number: 20040171065Abstract: The present invention relates to a method for display of proteinson spore surface and a method for improving protein with rapidity using the same, which comprises the steps of (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding a protein of interest, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the protein of interest, (ii) transforming a host cell with the vector for spore surface display; (iii) displaying the protein of interest on a surface of a spore of the host cell; and (iv) recovering the spore displaying on its surface the protein of interest.Type: ApplicationFiled: June 5, 2003Publication date: September 2, 2004Inventors: June-Hyung Kim, Byung-Gee Kim, Soo-Keun Choi, Heung-Chae Jung, Jae-Gu Pan
-
Publication number: 20030073109Abstract: The present invention relates to a high throughput screening method for preparing a variant of catalytic polypeptide capable of catalyzing a chemical reaction.Type: ApplicationFiled: June 28, 2002Publication date: April 17, 2003Inventors: Jae-Gu Pan, Heung-Chae Jung, Yong-Sung Kim
-
Publication number: 20020115146Abstract: The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment for a gene that encodes a desired protein, wherein said promoter has an inductive activity for transcription during the resting stage of cell growth and also the induction of said protein expression can be controlled by varying culturing conditions.Type: ApplicationFiled: September 5, 2001Publication date: August 22, 2002Inventors: Jae-Gu Pan, Heung-Chae Jung, Sooan Shin
-
Patent number: 6071725Abstract: The present invention relates to surface anchoring vectors, a method for preparation of foreign proteins onto a cell surface and use thereof, which uses outer cell membrane protein, ice nucleation protein (NIP) derived from Pseudomonas syringae, a gram-negative bacterium.Type: GrantFiled: December 2, 1997Date of Patent: June 6, 2000Assignee: Korea Institute of Science and TechnologyInventors: Jae Gu Pan, Heung Chae Jung, Seung Hwan Park, Moon Hi Han, Young Hoon Park