Abstract: The present invention provides methods for differentiating brain microvascular endothelial cells having barrier properties and a mature immune phenotype for use in making an in vitro blood-brain barrier (BBB) model. Further, a BBB model having barrier properties and a mature immune phenotype is provided.

Abstract: An information processing apparatus performs predetermined game processing based on operation inputs from a plurality of operation devices and generates a game image based on the game processing. Then, in accordance with the number of the operation devices used for the game processing, the information processing apparatus generates a game image for multiplayer play using a split screen, and performs a restriction process for, in a case where an output destination to which the game image is to be output is a display, placing a restriction so that multiplayer play is not performed by players exceeding a predetermined number of players, and in a case where the output destination to which the game image is to be output is an external display, not placing the restriction.

Abstract: An information processing apparatus displays an image on a display, directly communicates wirelessly with another information processing apparatus, and also receives a plurality of operation input data from a plurality of operation devices. Then, the information processing apparatus performs game processing for a communication game based on the plurality of operation input data and received information, and based on the game processing, generates, by screen splitting, game images including images on which operations on the plurality of respective operation devices are reflected, and causes the game images to be displayed on the display.

Abstract: An information processing apparatus displays an image on a display, directly communicates wirelessly with another information processing apparatus, and also receives a plurality of operation input data from a plurality of operation devices. Then, the information processing apparatus performs game processing for a communication game based on the plurality of operation input data and received information, and based on the game processing, generates, by screen splitting, game images including images on which operations on the plurality of respective operation devices are reflected, and causes the game images to be displayed on the display.

Abstract: An information processing apparatus displays an image on a display, directly communicates wirelessly with another information processing apparatus, and also receives a plurality of operation input data from a plurality of operation devices. Then, the information processing apparatus performs game processing for a communication game based on the plurality of operation input data and received information, and based on the game processing, generates, by screen splitting, game images including images on which operations on the plurality of respective operation devices are reflected, and causes the game images to be displayed on the display.

Abstract: An information processing apparatus displays an image on a display, directly communicates wirelessly with another information processing apparatus, and also receives a plurality of operation input data from a plurality of operation devices. Then, the information processing apparatus performs game processing for a communication game based on the plurality of operation input data and received information, and based on the game processing, generates, by screen splitting, game images including images on which operations on the plurality of respective operation devices are reflected, and causes the game images to be displayed on the display.

Abstract: An information processing apparatus performs predetermined game processing based on operation inputs from a plurality of operation devices and generates a game image based on the game processing. Then, in accordance with the number of the operation devices used for the game processing, the information processing apparatus generates a game image for multiplayer play using a split screen, and performs a restriction process for, in a case where an output destination to which the game image is to be output is a display, placing a restriction so that multiplayer play is not performed by players exceeding a predetermined number of players, and in a case where the output destination to which the game image is to be output is an external display, not placing the restriction.

Abstract: A bactericidal and fungicidal solution is made by a method wherein an aqueous solution containing ammonium chloride or a chloride of an alkali metal or alkaline earth metal as chloride ions is prepared, a silver salt or metallic silver is added to the aqueous solution and silver ions are dissolved as a chloro complex salt. The aqueous solution exhibits a bactericidal effect at a concentration of silver ions contained as the chloro complex salt of 0.05 mg/liter or more and a bactericidal and fungicidal effect at a concentration of 0.5 mg/liter or more.