Abstract: The present invention has its object to provide a material for separating stem cell and a filter for separating stem cell, each is capable of selectively separating and recovering, in a simple and easy manner, stem cells from body fluids or biological tissue-derived treated fluids, a method for separating and recovering stem cells, and stem cells obtained by such method. The present invention is a material for separating stem cell which has a density K of 1.0×104?K?1.0×106 and a fiber diameter of 3 to 40 ?m; a filter for separating stem cell which comprises the material for separating stem cell as packed in a container having a fluid inlet port and a fluid outlet port; a method of separating and recovering stem cells which comprises using the material for separating stem cell or the filter for separating stem cell; and a method of producing a multipotent cell fraction.

Abstract: The present invention has its object to provide a material for separating stem cell and a filter for separating stem cell, each is capable of selectively separating and recovering, in a simple and easy manner, stem cells from body fluids or biological tissue-derived treated fluids, a method for separating and recovering stem cells, and stem cells obtained by such method. The present invention is a material for separating stem cell which has a density K of 1.0×104?K?1.0×106 and a fiber diameter of 3 to 40 ?m; a filter for separating stem cell which comprises the material for separating stem cell as packed in a container having a fluid inlet port and a fluid outlet port; a method of separating and recovering stem cells which comprises using the material for separating stem cell or the filter for separating stem cell; and a method of producing a multipotent cell fraction.

Abstract: The present invention has its object to provide a material for separating stem cell and a filter for separating stem cell, each is capable of selectively separating and recovering, in a simple and easy manner, stem cells from body fluids or biological tissue-derived treated fluids, a method for separating and recovering stem cells, and stem cells obtained by such method. The present invention is a material for separating stem cell which has a density K of 1.0×104?K?1.0×106 and a fiber diameter of 3 to 40 ?m; a filter for separating stem cell which comprises the material for separating stem cell as packed in a container having a fluid inlet port and a fluid outlet port; a method of separating and recovering stem cells which comprises using the material for separating stem cell or the filter for separating stem cell; and a method of producing a multipotent cell fraction.

Abstract: This invention relates to a granulocyte adsorbent comprising a polymer compound having an unsubstituted hydroxyl group and a hydroxyl group in which H has been substituted with at least one substituent R, a method for producing such adsorbent, a device for adsorbing granulocytes and a system for removing granulocytes using such adsorbent, and body fluid or a cell suspension from which granulocytes have been removed using such adsorbent.

Abstract: The present invention relates to a method of body fluid treatment for relieving the condition of inhibited lymphocyte proliferation which comprises bringing a mammalian body fluid into contact with an adsorbent comprising a high-molecular compound having a water contact angle within the range of 40° to 98° in the presence of a divalent cation chelating agent in the manner of extracorporeal circulation, or comprises bringing a mammalian body fluid into contact with an active carbon-containing adsorbent in the manner of extracorporeal circulation; and relates to a system for extracorporeal circulation which comprises a body fluid transfer pump, an anticoagulant infusion pump, and a device comprising a container wherein an adsorbent comprising a high-molecular compound having a water contact angle within the range of 40° to 98° is contained, or comprises a body fluid transfer pump, and a device comprising a container wherein an active carbon-containing adsorbent is contained.

Abstract: The present invention has its object to provide a material for separating stem cell and a filter for separating stem cell, each is capable of selectively separating and recovering, in a simple and easy manner, stem cells from body fluids or biological tissue-derived treated fluids, a method for separating and recovering stem cells, and stem cells obtained by such method. The present invention is a material for separating stem cell which has a density K of 1.0×104?K?1.0×106 and a fiber diameter of 3 to 40 ?m; a filter for separating stem cell which comprises the material for separating stem cell as packed in a container having a fluid inlet port and a fluid outlet port; a method of separating and recovering stem cells which comprises using the material for separating stem cell or the filter for separating stem cell; and a method of producing a multipotent cell fraction.

Abstract: The present invention provides a scaffold in which cells can be stably retained and grafted in a uniform distribution state in the culture, preferable proliferation ability and viability can be secured, and particularly in the case of cartilage, fixation treatment such as suture can be carried out in the transplantation into affected parts after the culture, and the mechanical strength is provided sustainable for (weighted) compression at the initial stage of transplantation. The present invention relates to a 3-dimensional porous scaffold for tissue regeneration which comprises a structure composed of vertically long-shaped pores having a pore diameter of not less than 10 ?m to not more than 500 ?m and pore length of not less than 20 ?m to not more than 1 cm being juxtaposedly arranged obtained by a production process comprising rapid freeze-drying as a key technology.

Abstract: The present invention provides a method and an apparatus for selectively removing non-target cells from a mixed cell population of different types of cells to selectively separate target cells. Individual cells or cell groups of at least two cells from a mixed cell population of different types of cells are placed on a certain two-dimensional, coordinate system. Then a distinction is made among the individual cells or cells in the cell groups, based on the shape or size of the cells or by using a cell marker. Physical energy such as laser light is applied to the positions or regions occupied by non-target cells or cell groups including non-target cells in order to selectively kill the non-target cells or cause dysfunction of the non-target cells.

Abstract: This invention relates to a granulocyte adsorbent comprising a polymer compound having an unsubstituted hydroxyl group and a hydroxyl group in which H has been substituted with at least one substituent R, a method for producing such adsorbent, a device for adsorbing granulocytes and a system for removing granulocytes using such adsorbent, and body fluid or a cell suspension from which granulocytes have been removed using such adsorbent.

Abstract: It is an object of the present invention to provide a method for detecting nerve stem cells/progenitor cells among a tissue or a mass of cells as constituted of a plurality of cell species, and a method for screening for a drug functioning as a nerve differentiation factor or the like by using the above-mentioned detection method. It was found in this invention that the expression of the NC1 gene was high in undifferentiated nerve stem cells and said expression decreased with an advance of differentiation. Furthermore in this invention, by using the NC1 gene, the product thereof (NC1 protein) and the base sequences or the amino acid sequences thereof, antibodies or the like, it became possible to detect nerve stem cells/progenitor cells by way of various methods, so that a novel method for screening for a drug functioning as a nerve differentiation factor or the like was provided.

Abstract: The present invention provides a scaffold in which cells can be stably retained and grafted in a uniform distribution state in the culture, preferable proliferation ability and viability can be secured, and particularly in the case of cartilage, fixation treatment such as suture can be carried out in the transplantation into affected parts after the culture, and the mechanical strength is provided sustainable for (weighted) compression at the initial stage of transplantation. The present invention relates to a 3-dimensional porous scaffold for tissue regeneration which comprises a structure composed of vertically long-shaped pores having a pore diameter of not less than 10 ?m to not more than 500 ?m and pore length of not less than 20 ?m to not more than 1 cm being juxtaposedly arranged obtained by a production process comprising rapid freeze-drying as a key technology.

Abstract: The object of the present invention is to detect and select insulin-producing cells capable of proliferating, and further to differentiate/proliferate insulin-producing cells and precursory thereof, or cells related thereto. The present invention was made by finding three species of novel genes by detecting genes specifically expressed in pancreases of PHHI patients which are regarded as a model of spontaneous proliferation of pancreatic ? cells, and searching for base sequence data bases. According to the present invention, proliferating insulin-producing cells can be detected using these genes, gene products or gene sequences by, for example, Northern analysis or RT-PCR. Furthermore, these genes can be differentiated into insulin-producing cells by introducing these genes into appropriate cells in genetic engineering manner.

Abstract: A thin film magnetic head includes an upper magnetic pole maintaining a constant thickness in a range determined by the so-called gap depth. A thinner film portion is defined in the upper magnetic pole in a range rearward of the range determined by the gap depth. The thinner film portion has a reduced thickness smaller than the constant thickness. The upper magnetic pole starts to enlarge the core width in the lateral direction at the rear end of the region determined by the so-called neck height. The neck height smaller than the gap depth serves to establish a reduction in the thickness of the upper magnetic pole after the upper magnetic pole gets larger in the lateral direction. A magnetic saturation can reliably be prevented at the thinner film portion in the upper magnetic pole. The front end of the upper magnetic pole is allowed to receive a larger quantity of a magnetic flux. It is possible to reliably enhance a magnetic field for recordation in the thin film magnetic head.

Abstract: A thin film magnetic head includes an upper magnetic pole maintaining a constant thickness in a range determined by the so-called gap depth. A thinner film portion is defined in the upper magnetic pole in a range rearward of the range determined by the gap depth. The thinner film portion has a reduced thickness smaller than the constant thickness. The upper magnetic pole starts to enlarge the core width in the lateral direction at the rear end of the region determined by the so-called neck height. The neck height smaller than the gap depth serves to establish a reduction in the thickness of the upper magnetic pole after the upper magnetic pole gets larger in the lateral direction. A magnetic saturation can reliably be prevented at the thinner film portion in the upper magnetic pole. The front end of the upper magnetic pole is allowed to receive a larger quantity of a magnetic flux. It is possible to reliably enhance a magnetic field for recordation in the thin film magnetic head.

Abstract: A magnetic head having an accurately defined minute core width is provided. The magnetic head comprises a pair of soft-magnetic poles which are located adjacent to a magnetic disk and opposed to and apart from each other with a predetermined distance along a predetermined direction, and generates a magnetic field therebetween to magnetize the magnetic disk, wherein the upper magnetic pole 3 of the two magnetic poles has, at one end which is adjacent to the magnetic disk, a tip of a block form that has a front wall which is adjacent to the magnetic disk and a pair of side walls which extend in a direction perpendicular to the front wall as well as in the above-described predetermined direction, with the tip having an upper non-magnetic regions 3—2 which has been formed by demagnetization by introducing impurity through each of the two side walls to a predetermined depth.

Abstract: A sheep LFA-3 protein and derivertires thereof, and derivertives of a human LFA-3 protein, gene coding for the proteins, and processes for preparing the proteins. Said proteins have high affinity for human T-cells.

Abstract: An apparatus for separating piled iron sheets one by one has a plurality of magnets arranged vertically with the same magnetic poles thereof facing each other. Magnetic fluxes generated from the magnets react with each other at a position between the magnets and flow horizontally and then vertically, with the result that opposite, vertically separating forces act on two superposed iron sheets.

Abstract: A DNA sequence on which a chromosomal DNA sequence coding for human interferon-.gamma. (HuIFN-.gamma.) and having a TATA box is ligated to a sequence of a promoter. Cell cultures transformed with said DNA revealed a higher expression of HuIFN- than cell cultures transformed with a DNA which does not have such TATA box. When transformed with said DNA, cell cultures produced HuIFN-.gamma. in a serum free medium. Transformed cell cultures derived from blood cells of HuIFN-.gamma. resistant human cell-line also could produce HuIFN-.gamma..

Abstract: A movable yoke-type lifting magnet device including a main core wound with a coil and at least one yoke magnetically coupled to the core. The movable mechanism of the yoke enables the yoke to freely follow the contour of a workpiece to be lifted. An auxiliary core may be provided between each said at least one yoke to provide a yoke-type lifting magnet device. The auxiliary core greatly increases the magnetic forces induced between the cores and the yoke. Combination of the magnetic coupling between the yokes and the core and the provision of the auxiliary core provides a movable yoke-type lifitng magnet device wherein the yoke can freely follow the contour of a workpiece and the magnetic forces between the cores and the yoke are greatly increased. Such a combination prevents the yoke from slipping relative to the cores and stable lifting is possible.

Abstract: A process and circuit arrangement for exciting a solenoid coil to operate an iron core. Power from an AC power source is full-wave rectified, and then allowed to flow to a solenoid coil by a switch element located between the full-wave rectification circuit and the solenoid coil, which coil is made electrically conductive only for each short period of time corresponding to the top and its vicinities of the voltage wave-form of the rectified power so that the current is allowed to flow to the solenoid coil. Therefore, no current flows to the solenoid for each period of time corresponding to the trough and its vicinities of voltage wave-form of the rectified power. Thus, excitation and non-excitation of the solenoid is continuously alternated to give a dither effect to the iron core associated with the solenoid coil.