Patents by Inventor Hilla Locker-Giladi

Hilla Locker-Giladi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Expression vectors containing .lambda.P.sub.L promoter and T.sub.1 T.sub.2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods
    Patent number: 5759816
    Abstract: An improved vector upon introduction into a suitable host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a desired gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site; an ATG initiation codon or DNA which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a second restriction enzyme site for inserting the gene in phase with the ATG codon; a T.sub.1 T.sub.2 rRNA transcription termination sequence; an origin of replication and a gene associated with a selectable or identifiable phenotypic trait manifested when the vector is present in the host. The distance between the 3' end of the P.sub.L O.sub.
    Type: Grant
    Filed: May 25, 1995
    Date of Patent: June 2, 1998
    Assignee: Bio-Technology General Corp.
    Inventors: Amos B. Oppenheim, Avigdor Levanon, Hilla Locker-Giladi, Marian Gorecki, Tikva Vogel
  • Expression vectors containing lambdapl promoter, T.sub.1 T.sub.2 RRNA termination sequence, and origin of replication derived from a constitutive high copy number plasmid, and hosts containing the plasmids
    Patent number: 5151364
    Abstract: An improved vector upon introduction into a suitable host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a desired gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site for transcribing mRNA; and ATG initiation codon or DNA which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a second restriction enzyme site for inserting the gene in phase with the ATG codon; a T.sub.1 T.sub.
    Type: Grant
    Filed: January 14, 1992
    Date of Patent: September 29, 1992
    Assignee: Bio-Technology General Corp.
    Inventors: Avigdor Levanon, Amos B. Oppenheim, Hilla Locker-Giladi
  • Stabilized expression vectors containing lambda pl promoter and the gene for the CI434 repressor, plasmids containing the vectors, hosts containing the plasmids and related methods
    Patent number: 5147789
    Abstract: An improved vector upon introduction into a suitable host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a desired gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal bindig site; an ATG initiation codon or DNA which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a second restriction enzyme site for inserting the gene in phase with the ATG codon; a T.sub.1 T.sub.2 rRNA transcription termination sequence; an origin of replication; and a fragment designated CI.sup.434 on which is included the gene for the repressor protein and its associated promoter and operator.
    Type: Grant
    Filed: October 22, 1991
    Date of Patent: September 15, 1992
    Assignee: Bio-Technology General Corp.
    Inventors: Amos B. Oppenheim, Hilla Locker-Giladi
  • Expression vectors containing .lambda.P.sub.L promoter, T.sub.1 T.sub.2 rRNA termination sequence and origin of replication derived from a constitutive high copy number plasmid hosts and related methods
    Patent number: 5081020
    Abstract: An improved vector upon introduction into a suitable host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a desired gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site for transcribing mRNA; an ATG initiation codon or DNA which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a second restriction enzyme site for inserting the gene in phase with the ATG codon; a T.sub.1 T.sub.
    Type: Grant
    Filed: July 18, 1988
    Date of Patent: January 14, 1992
    Assignee: Bio-Technology General Corp.
    Inventors: Avigdor Levanon, Amos B. Oppenheim, Hilla Locker-Giladi