Patents by Inventor Hiroshi Ezura
Hiroshi Ezura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220298524Abstract: The present application provides: a fruit-bearing plant exhibiting parthenocarpy or a potion thereof including a mutation in a Solyc10g038170 gene or ortholog thereof, the mutation causing deficiency or reduction in expression of a protein encoded by the Solyc10g038170 gene or ortholog thereof; a method for producing a parthenocarpic fruit-bearing plant or a potion thereof; and a method for selecting a parthenocarpic fruit-bearing plant or a potion thereof.Type: ApplicationFiled: August 28, 2020Publication date: September 22, 2022Applicant: UNIVERSITY OF TSUKUBAInventors: Tohru ARIIZUMI, Hiroshi EZURA, Keiichiro HARADA, Yoshihito SHINOZAKI, Ryoichi YANO, U RIKU
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Patent number: 11279941Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.Type: GrantFiled: January 19, 2017Date of Patent: March 22, 2022Assignee: RIKENInventors: Keiji Numata, Yoko Horii, Takeshi Yoshizumi, Taku Demura, Yutaka Kodama, Takashi Yamamoto, Tetsushi Sakuma, Kenji Miura, Hiroshi Ezura
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Publication number: 20210108218Abstract: An expression system is provided, including a first nucleic acid fragment containing a long intergenic region (LIR) derived from geminivirus, a small intergenic region (SIR) derived from geminivirus, and an expression cassette of a target protein linked between the LIR and the SIR and a second nucleic acid fragment containing an expression cassette of a Rep/RepA protein derived from geminivirus, in which the expression cassette of the target protein includes a promoter, a nucleic acid fragment encoding the target protein, and two or more linked terminators in this order.Type: ApplicationFiled: March 6, 2018Publication date: April 15, 2021Applicant: University of TsukubaInventors: Kenji MIURA, Hiroshi EZURA, Ken HOSHIKAWA
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Publication number: 20200318125Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.Type: ApplicationFiled: January 19, 2017Publication date: October 8, 2020Applicant: RIKENInventors: Keiji NUMATA, Yoko HORII, Takeshi YOSHIZUMI, Taku DEMURA, Yutaka KODAMA, Takashi YAMAMOTO, Tetsushi SAKUMA, Kenji MIURA, Hiroshi EZURA
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Patent number: 10745710Abstract: The present invention relates to a plant having an improved sugar content in fruit compared with its wild type plant, which has a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein. The present invention also relates to a parthenocarpic plant having a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein.Type: GrantFiled: August 5, 2016Date of Patent: August 18, 2020Assignee: UNIVERSITY OF TSUKUBAInventors: Hiroshi Ezura, Tohru Ariizumi, Jun-ichiro Masuda, Yoshihiro Okabe
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Patent number: 10385357Abstract: Provided is a plant such as tomato, in which plant vigor has been increased and heat resistance has further been imparted. A plant such as tomato can be obtained, which has increased the stem diameter as well as the plant height and has become heat resistant by having a mutant Della protein, in which a leucine corresponding to the leucine at position 567 in SEQ ID NO: 2 in the amino acid sequence of the Della protein has been replaced by another amino acid, preferably phenylalanine.Type: GrantFiled: November 7, 2014Date of Patent: August 20, 2019Assignees: UNIVERSITY OF TSUKUBA, RIKENInventors: Hiroshi Ezura, Tohru Ariizumi, Yoshihito Shinozaki, Junji Kimbara, Miyako Kusano, Atsushi Fukushima, Kazuki Saito
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Patent number: 10349611Abstract: A heat-tolerant tomato plant exhibiting a high capacity for developing seed-containing fruits under high temperature conditions is provided. The present invention relates to a method for producing a heat-tolerant tomato plant comprising introducing a genetic mutation into a tomato plant, wherein the mutation improves the pollen viability and the capacity for developing seed-containing fruits under high temperature conditions compared with wild-type plant; and a heat-tolerant tomato plant into which the mutation has been introduced.Type: GrantFiled: September 25, 2015Date of Patent: July 16, 2019Assignee: UNIVERSITY OF TSUKUBAInventors: Hiroshi Ezura, Ken Hoshikawa, Shoma Fukumoto, Sayaka Ooshima, Mina Aiba
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Publication number: 20190110420Abstract: The present invention relates to a plant having an improved sugar content in fruit compared with its wild type plant, which has a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein. The present invention also relates to a parthenocarpic plant having a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein.Type: ApplicationFiled: August 5, 2016Publication date: April 18, 2019Inventors: Hiroshi EZURA, Tohru ARIIZUMI, Jun-ichiro MASUDA, Yoshihiro OKABE
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Patent number: 9944936Abstract: The present invention relates to an Agrobacterium having improved gene transfer efficiency. The present invention provides a transformed Agrobacterium which harbors a foreign GABA transaminase gene and exhibits improved gene transfer efficiency, and a method for producing a transformed plant using the same.Type: GrantFiled: February 7, 2014Date of Patent: April 17, 2018Assignee: UNIVERSITY OF TSUKUBAInventors: Hiroshi Ezura, Koji Nakamura, Satoko Nonaka, Tatsuhiko Someya, Sha Zhou
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Publication number: 20170292130Abstract: A heat-tolerant tomato plant exhibiting a high capacity for developing seed-containing fruits under high temperature conditions is provided. The present invention relates to a method for producing a heat-tolerant tomato plant comprising introducing a genetic mutation into a tomato plant, wherein the mutation improves the pollen viability and the capacity for developing seed-containing fruits under high temperature conditions compared with wild-type plant; and a heat-tolerant tomato plant into which the mutation has been introduced.Type: ApplicationFiled: September 25, 2015Publication date: October 12, 2017Applicant: UNIVERSITY OF TSUKUBAInventors: Hiroshi EZURA, Ken HOSHIKAWA, Shoma FUKUMOTO, Sayaka OOSHIMA, Mina AIBA
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Publication number: 20160264989Abstract: Provided is a plant such as tomato, in which plant vigor has been increased and heat resistance has further been imparted. A plant such as tomato can be obtained, which has increased the stem diameter as well as the plant height and has become heat resistant by having a mutant Della protein, in which a leucine corresponding to the leucine at position 567 in SEQ ID NO: 2 in the amino acid sequence of the Della protein has been replaced by another amino acid, preferably phenylalanine.Type: ApplicationFiled: November 7, 2014Publication date: September 15, 2016Inventors: Hiroshi EZURA, Tohru ARIIZUMI, Yoshihito SHINOZAKI, Junji KIMBARA, Miyako KUSANO, Atsushi FUKUSHIMA, Kazuki SAITO
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Publication number: 20150368658Abstract: The present invention relates to an Agrobacterium having improved gene transfer efficiency. The present invention provides a transformed Agrobacterium which harbors a foreign GABA transaminase gene and exhibits improved gene transfer efficiency, and a method for producing a transformed plant using the same.Type: ApplicationFiled: February 7, 2014Publication date: December 24, 2015Applicant: UNIVERSITY OF TSUKUBAInventors: Hiroshi EZURA, Koji NAKAMURA, Satoko NONAKA, Tatsuhiko SOMEYA, Sha ZHOU
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Publication number: 20140020134Abstract: The present invention relates to a fruit-specific promoter, which is suitable for the expression in a broader range of developmental stages of a fruit. Provided is a fruit-specific promoter DNA, which consists of a nucleotide sequence having 85% or more identity with the nucleotide sequence as shown in SEQ ID NO: 1 or 2 and has promoter activity in mature-green fruits.Type: ApplicationFiled: March 30, 2011Publication date: January 16, 2014Applicants: INPLANTA INNOVATIONS INC., UNIVERSITY OF TSUKUBA, KAZUSA DNA RESEARCH INSTITUTEInventors: Nobuyo Itou, Hirofumi Kuroda, Ken-ichi Takane, Kou Aoki, Daisuke Shibata, Hiroshi Ezura, Kyoko Tanase
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Publication number: 20090205068Abstract: The present invention relates to: a method for producing a transgenic plant expressing miraculin, comprising introducing a miraculin gene into a useful plant that can be easily produced and cultivated throughout the year using plant molecular breeding technology; a transgenic plant expressing miraculin, which is produced by the method; and the use thereof. The present invention also relates to a food additive, a food or drink, or an antidiabetic drug, which contains miraculin that is produced by the transgenic plant.Type: ApplicationFiled: August 4, 2005Publication date: August 13, 2009Applicants: Hiroshi EZURA, INPLANTA INNOVATIONS INCInventors: Hiroshi Ezura, Hyeon-Jin Sun, Masanobu Kirita, Takanari Ichikawa, Nobuyo Nishizaki