Patents by Inventor Hiroyuki Kanamaru
Hiroyuki Kanamaru has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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PRODUCTION METHOD FOR IMMOBILIZED MICROORGANISMS AND PRODUCTION METHOD FOR AMINO ACID USING THE SAME
Publication number: 20220025351Abstract: An object of the present invention is to provide a method for producing an immobilized microorganism having high filtration properties, and to provide a method for producing an amino acid using the immobilized microorganism. A method for producing an immobilized microorganism is characterized in that a microorganism is contacted with carboxymethyl cellulose sodium salt and then contacted with polyethylenimine and an alkane dial after the first contact with carboxymethyl cellulose sodium salt.Type: ApplicationFiled: September 3, 2019Publication date: January 27, 2022Applicant: KANEKA CORPORATIONInventors: Mirai TANAKA, Hiroyuki KANAMARU, Noriyuki ITO, Hiroaki YASUKOUCHI -
Patent number: 10435732Abstract: The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32° C. or higher a Brevibacillus bacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32° C. after the high-temperature culture step.Type: GrantFiled: June 9, 2015Date of Patent: October 8, 2019Assignee: KANEKA CORPORATIONInventors: Hiroyuki Kanamaru, Teruaki Takesue, Osamu Odawara, Takeyuki Tsuchidate
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Publication number: 20170211116Abstract: The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32° C. or higher a Brevibacillus bacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32° C. after the high-temperature culture step.Type: ApplicationFiled: June 9, 2015Publication date: July 27, 2017Applicant: Kaneka CorporationInventors: Hiroyuki KANAMARU, Teruaki TAKESUE, Osamu ODAWARA, Takeyuki TSUCHIDATE
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Patent number: 9493762Abstract: An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them.Type: GrantFiled: September 28, 2012Date of Patent: November 15, 2016Assignee: KANEKA CORPORATIONInventors: Masutoshi Nojiri, Hiroyuki Kanamaru, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
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Patent number: 9273332Abstract: The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.Type: GrantFiled: October 11, 2007Date of Patent: March 1, 2016Assignee: Kaneka CorporationInventors: Hiroyuki Kanamaru, Makoto Ueda, Hirokazu Nanba
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Patent number: 9267116Abstract: The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.Type: GrantFiled: December 8, 2009Date of Patent: February 23, 2016Assignee: KANEKA CORPORATIONInventors: Hiroyuki Kanamaru, Ryuuji Miki, Makoto Ueda, Hironori Nanba, Yoshihiko Yasohara
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Publication number: 20140335575Abstract: An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them.Type: ApplicationFiled: September 28, 2012Publication date: November 13, 2014Inventors: Masutoshi Nojiri, Hiroyuki Kanamaru, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
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Patent number: 8227228Abstract: The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.Type: GrantFiled: August 2, 2006Date of Patent: July 24, 2012Assignee: Kaneka CorporationInventors: Hiroyuki Kanamaru, Makoto Ueda, Ryuuji Miki, Hirokazu Nanba
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Publication number: 20110281309Abstract: The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.Type: ApplicationFiled: December 8, 2009Publication date: November 17, 2011Applicant: KANEKA CORPORATIONInventors: Hiroyuki Kanamaru, Ryuuji Miki, Makoto Ueda, Hironori Nanba, Yoshihiko Yasohara
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Publication number: 20110086396Abstract: The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.Type: ApplicationFiled: August 2, 2006Publication date: April 14, 2011Applicant: Kaneka CorporationInventors: Hiroyuki Kanamaru, Makoto Ueda, Ruuji Miki, Hirokazu Nanba
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Publication number: 20100028959Abstract: The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.Type: ApplicationFiled: October 11, 2007Publication date: February 4, 2010Applicant: Kaneka CorporationInventors: Hiroyuki Kanamaru, Makoto Ueda, Hirokazu Nanba
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Patent number: 5244985Abstract: Disclosed are an epoxidized polyester which comprises, per molecule, at least three bifunctional groups each having the general formula ##STR1## wherein R.sup.1 and R.sup.2 are the same or different and each is a hydrogen atom or an alkyl group containing 1 to 5 carbon atoms, or R.sup.1 and R.sup.2 taken together represent an endomethylene group, and which has a number average molecular weight of about 1,000 to about 10,000, as well as processes for preparing the epoxidized polyester.Type: GrantFiled: March 25, 1992Date of Patent: September 14, 1993Assignee: New Japan Chemical Co., Ltd.Inventors: Tomio Nobe, Hiroyuki Kanamaru, Harutomo Nomoto, Shoji Tani
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Patent number: 4983391Abstract: An insecticide for protecting a fibrous material from the damage by an insect, which comprises a carbamic ester of the formula: ##STR1## wherein R.sub.1 and R.sub.2 are, the same or different, each a hydrogen atom or a lower alkyl group and R.sub.3 is a lower alkyl group as an active ingredient.Type: GrantFiled: August 9, 1989Date of Patent: January 8, 1991Assignee: S.T. Chemical Co., Ltd.Inventors: Ryonosuke Muneyuki, Hiroyuki Kanamaru
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Patent number: 4912137Abstract: An insecticide which comprises an oxalic ester of the formula: ##STR1## wherein R.sub.1 and R.sub.2 are, the same or different, each a hydrogen atom or a C.sub.1 -C.sub.4 alkyl group but at least one of them is a C.sub.1 -C.sub.4 alkyl group.Type: GrantFiled: July 12, 1988Date of Patent: March 27, 1990Assignee: S. T. Chemical Co., Ltd.Inventors: Ryonosuke Muneyuki, Hiroyuki Kanamaru