Patents by Inventor Hisaaki Mihara

Hisaaki Mihara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Dehydrogenase and a gene encoding the same
    Patent number: 7452704
    Abstract: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.
    Type: Grant
    Filed: May 23, 2006
    Date of Patent: November 18, 2008
    Assignee: Mitsubishi Chemical Corporation
    Inventors: Nobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda
  • Dehydrogenase and a gene encoding the same
    Patent number: 7115735
    Abstract: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30 ° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.
    Type: Grant
    Filed: August 27, 2004
    Date of Patent: October 3, 2006
    Assignee: Mitsubishi Chemical Corporation
    Inventors: Nobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda
  • Novel dehydrogenase and a gene encodign the same
    Publication number: 20060205045
    Abstract: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.
    Type: Application
    Filed: May 23, 2006
    Publication date: September 14, 2006
    Applicant: Mitsubishi Chemical Corporation
    Inventors: Nobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda
  • Novel dehydrogenase and a gene encoding the same
    Publication number: 20050124040
    Abstract: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.
    Type: Application
    Filed: August 27, 2004
    Publication date: June 9, 2005
    Applicant: Mitsubishi Chemical Corporation
    Inventors: Nobuyoshi Esaki, Hisaaki Mihara, Mari Hara, Makoto Ueda