Patents by Inventor Holger Engel
Holger Engel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10843193Abstract: A microfluidic system is presented that includes a cartridge and a container. The cartridge includes a plurality of microfluidic channels coupled to one or more chambers. The container holds dry chemicals and includes a housing with a first opening and a second opening smaller than the first opening. The container is designed to be inserted into an opening of the cartridge, such that the container is independently secured within the opening. The insertion of the container allows for the container to be fluidically coupled with a microfluidic channel of the plurality of microfluidic channels via the second opening.Type: GrantFiled: May 23, 2019Date of Patent: November 24, 2020Assignee: STAT-DIAGNOSTICA & INNOVATION, S.L.Inventors: Holger Engel, Jordi Carrera Fabra
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Publication number: 20190275525Abstract: A microfluidic system is presented that includes a cartridge and a container. The cartridge includes a plurality of microfluidic channels coupled to one or more chambers. The container holds dry chemicals and includes a housing with a first opening and a second opening smaller than the first opening. The container is designed to be inserted into an opening of the cartridge, such that the container is independently secured within the opening. The insertion of the container allows for the container to be fluidically coupled with a microfluidic channel of the plurality of microfluidic channels via the second opening.Type: ApplicationFiled: May 23, 2019Publication date: September 12, 2019Inventors: Holger ENGEL, Jordi CARRERA FABRA
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Publication number: 20190217292Abstract: A microfluidic system is presented that includes a cartridge, a container, and a lid for the container. The cartridge includes a plurality of microfluidic channels coupled to one or more chambers. The container holds dry chemicals and includes a housing with a first opening and a second opening smaller than the first opening. The container is designed to be inserted into an opening of the cartridge, such that the container is independently secured within the opening. The insertion of the container allows for the container to be fluidically coupled with a microfluidic channel of the plurality of microfluidic channels via the second opening. The lid includes a column that extends from the lid into the container.Type: ApplicationFiled: January 18, 2018Publication date: July 18, 2019Inventors: Holger Engel, Jordi Carrera Fabra, Anna Comenges Casas, Ricard Martin Blanco, Martin Alan Lee, Diane Rachel Lee
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Patent number: 10300486Abstract: A microfluidic system is presented that includes a cartridge and a container. The cartridge includes a plurality of microfluidic channels coupled to one or more chambers. The container holds dry chemicals and includes a housing with a first opening and a second opening smaller than the first opening. The container is designed to be inserted into an opening of the cartridge, such that the container is independently secured within the opening. The insertion of the container allows for the container to be fluidically coupled with a microfluidic channel of the plurality of microfluidic channels via the second opening.Type: GrantFiled: July 14, 2016Date of Patent: May 28, 2019Assignee: STAT-DIAGNOSTICA & INNOVATION, S.L.Inventors: Holger Engel, Jordi Carrera Fabra
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Patent number: 10011827Abstract: The method for isolating nucleic acids from a food sample comprising the following steps: a) obtaining a food enrichment culture; b) transferring a portion of the food enrichment culture into a reaction vessel thereby providing a food enrichment sample and providing a water-immiscible phase in contact with the food enrichment culture; c) lysing the food enrichment sample to provide a lysed sample; d) isolating nucleic acids from the lysed sample. Food enrichment culture samples are known to contain high concentrations of organic and/or liposoluble inhibitors. By contacting the enrichment culture sample with a water-immiscible phase before the actual DNA extraction procedure starts, part of the lipophilic inhibitors is expected to cross the phase interface due to an enhanced solubility in the organic phase and thereby become depleted.Type: GrantFiled: February 28, 2013Date of Patent: July 3, 2018Assignee: QIAGEN GmbHInventors: Janina Cramer, Sarah Fakih, Corinna Küppers, Holger Engel
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Patent number: 9611506Abstract: This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, whereby the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.Type: GrantFiled: November 12, 2015Date of Patent: April 4, 2017Assignee: QIAGEN, GMBHInventors: Holger Engel, Subrahmanyam Yerramilli, Martin Kreutz, Dirk Loeffert, Christian Korfhage
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Publication number: 20170014826Abstract: A microfluidic system is presented that includes a cartridge and a container. The cartridge includes a plurality of microfluidic channels coupled to one or more chambers. The container holds dry chemicals and includes a housing with a first opening and a second opening smaller than the first opening. The container is designed to be inserted into an opening of the cartridge, such that the container is independently secured within the opening. The insertion of the container allows for the container to be fluidically coupled with a microfluidic channel of the plurality of microfluidic channels via the second opening.Type: ApplicationFiled: July 14, 2016Publication date: January 19, 2017Inventors: Holger ENGEL, Jordi CARRERA FABRA
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Publication number: 20160130634Abstract: This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, whereby the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.Type: ApplicationFiled: November 12, 2015Publication date: May 12, 2016Inventors: Holger ENGEL, Subrahmanyam Yerramilli, Martin Kreutz, Dirk Loeffert, Christian Korfhage
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Patent number: 9328385Abstract: The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.Type: GrantFiled: February 20, 2012Date of Patent: May 3, 2016Assignees: Qiagen GmbH, Qiagen Manchester LimitedInventors: Francesca Di Pasquale, Holger Engel, Sascha Strauss, Nicola Jo Thelwell
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Patent number: 9309567Abstract: The present invention concerns a method for a polymerase chain reaction, in which a template nucleic acid, at least one primer, deoxyribonucleoside triphosphates as well as a DNA polymerase with proofreading activity are used. In addition, according to this invention, at least one target substrate is added to the polymerase chain reaction, whereby the efficiency of the DNA polymerase with proofreading activity is significantly increased. Any molecule that reduces or, in the optimal case, blocks the 3?,5?-exonuclease activity of the DNA polymerase used is suitable as target substrate. Technical solutions for the added substrate (target substrate) are in particular single stranded, linear oligonucleotides, hairpin oligonucleotides and RNA and DNA molecules. Furthermore, a kit is disclosed which comprise the required reagents for the implementation of the method according to the invention.Type: GrantFiled: September 28, 2006Date of Patent: April 12, 2016Assignee: QIAGEN GmbHInventors: Holger Engel, Ralf Peist
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Patent number: 9290815Abstract: The invention provides for a method for quantifying one or more nucleic acids of a genome in a sample comprising the steps of, (a) amplifying a first nucleic acid to be quantified, (b) determining the amount of said first nucleic acid by comparison of the amount of amplification product from said first nucleic acid with at least one amplification product from a second template nucleic acid, (c) wherein said second template nucleic acid was generated using whole genome amplification and wherein the starting concentration of the second template nucleic acid is known.Type: GrantFiled: February 21, 2012Date of Patent: March 22, 2016Assignee: Qiagen GMBHInventors: Francesca Di Pasquale, Holger Engel
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Patent number: 9217173Abstract: This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, wherein the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.Type: GrantFiled: August 13, 2007Date of Patent: December 22, 2015Assignee: QIAGEN GMBHInventors: Holger Engel, Subrahmanyam Yerramilli, Martin Kreutz, Dirk Loeffert, Christian Korfhage
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Publication number: 20150056624Abstract: The method for isolating nucleic acids from a food sample comprising the following steps: a) obtaining a food enrichment culture; b) transferring a portion of the food enrichment culture into a reaction vessel thereby providing a food enrichment sample and providing a water-immiscible phase in contact with the food enrichment culture; c) lysing the food enrichment sample to provide a lysed sample; d) isolating nucleic acids from the lysed sample. Food enrichment culture samples are known to contain high concentrations of organic and/or liposoluble inhibitors. By contacting the enrichment culture sample with a water-immiscible phase before the actual DNA extraction procedure starts, part of the lipophilic inhibitors is expected to cross the phase interface due to an enhanced solubility in the organic phase and thereby become depleted.Type: ApplicationFiled: February 28, 2013Publication date: February 26, 2015Inventors: Janina Cramer, Sarah Fakih, Corinna Küppers, Holger Engel
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Patent number: 8932831Abstract: The present invention concerns a method for inserting one or more tag sequences into a nucleic acid characterized by the following steps: (a) preparation of a template nucleic acid; (b) hybridization of at least one anchor sequence of at least one anchor oligonucleotide with one sequence section of the template nucleic acid; and (c) synthesization of a new strand of nucleic acid, which is partially complementary to the template nucleic acid and which contains a sequence complementary to the non-hybridized portion of the anchor oligonucleotide, e.g. to at least one tag sequence, on its 3? end.Type: GrantFiled: May 4, 2007Date of Patent: January 13, 2015Assignee: Qiagen GmbHInventors: Christian Korfhage, Holger Engel, Dirk Löffert, Ralf Peist
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Patent number: 8852863Abstract: The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.Type: GrantFiled: May 4, 2010Date of Patent: October 7, 2014Assignee: Qiagen GmbHInventors: Thomas Rothmann, Holger Engel, Ralf Himmelreich, Andy Wende, Rainer Dahlke
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Publication number: 20140234834Abstract: The invention provides for a method for quantifying one or more nucleic acids of a genome in a sample comprising the steps of (a) amplifying a first nucleic acid to be quantified, (b) determining the amount of said first nucleic acid by comparison of the amount of amplification product from said first nucleic acid with at least one amplification product from a second template nucleic acid, (c) wherein said second template nucleic acid was generated using whole genome amplification and wherein the starting concentration of the second template nucleic acid is known.Type: ApplicationFiled: February 21, 2012Publication date: August 21, 2014Applicant: Qiagen GmbHInventors: Francesca Di Pasquale, Holger Engel
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Publication number: 20140147843Abstract: The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.Type: ApplicationFiled: February 20, 2012Publication date: May 29, 2014Applicants: QIAGEN MANCHESTER LIMITED, QIAGEN GMBHInventors: Francesca Di Pasquale, Holger Engel, Sascha Strauss, Nicola Jo Thel Well
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Publication number: 20140057263Abstract: The invention relates to a method for detecting a nucleic acid of an organism in a composition, comprising the steps of, (i) amplifying the nucleic acid to be detected, (ii) during or after amplification, hybridizing to said nucleic acid to be detected a first probe that comprises an abasic site additionally optionally carrying a detectable label, (iii) wherein the position of the abasic site corresponds to a position in said nucleic acid to be detected, known to have a polymorphism in said organism, and wherein said nucleic acid is detected if hybridization occurs.Type: ApplicationFiled: March 21, 2012Publication date: February 27, 2014Applicant: QIAGEN GMBHInventors: Holger Engel, Alexander Azzawi, Francesca Di Pasquale
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Patent number: 8486628Abstract: The present invention is related to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample.Type: GrantFiled: July 30, 2010Date of Patent: July 16, 2013Assignee: Qiagen GmbHInventors: Dirk Loeffert, Christian Korfhage, Holger Engel
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Publication number: 20120252014Abstract: The present invention is related to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample.Type: ApplicationFiled: July 30, 2010Publication date: October 4, 2012Inventors: Dirk Loeffert, Christian Korfhage, Holger Engel