Abstract: A nucleic acid amplification blocker for detecting a low-abundance mutation sequence and an application thereof in detecting a low-abundance mutation sequence are provided. The nucleic acid amplification blocker is an oligonucleotide modified by locked nucleic acid (LNA), and the matching region of the nucleic acid amplification blocker is located between amplified sequences. The nucleic acid amplification blocker is completely complementary to wild-type gene sequence, and contains at least one mismatch with mutant sequence. The nucleic acid amplification blocker has a great difference in affinity with mutant nucleic acid sequence/wild-type nucleic acid sequence, so as to achieve the purpose of highly selective amplification/enrichment of mutant sequence in samples. The nucleic acid amplification blocker has more significant detection effect on deletion mutation and insertion mutation.

Abstract: An imaging system for multiplex detection of biomolecules, including at least a first optical path and a second optical path; the first optical path is from an excitation light source to an objective lens, and a filter set is provided between the excitation light source and the objective lens and is configured to guide excitation light from the excitation light source to the objective lens; the second optical path is from the objective lens to an image acquisition device, a reflector is provided between the objective lens and the image acquisition device and is configured to project the fluorescence received by the objective lens to the image acquisition device; the excitation light source includes at least a first excitation light source used for excitation of fluorescent signals and a second excitation light source used for excitation of light-initiated chemiluminescence signals The system simultaneously realizes fluorescence and light-initiated chemiluminescence imaging.

Abstract: A detection particle suitable for multiplex detection of biomolecules, including a microcarrier with an encoding function, detection microparticles connected to the microcarrier, where the detection microparticle is suitable for light-initiated chemiluminescent detection. A multifunctional detection particle integrating encoding identification, magnetic separation, and light-initiated chemiluminescent detection functions was prepared. And an imaging detection and analysis device for the multifunctional detection particle was provided. The provided detection particle suitable for multiplex detection of biomolecules can realize a highly sensitive, fast, and washing-free multiplex detection method, which greatly increases the types of biomolecules that can be detected by a single reaction, and can remove unreacted signal labeled molecules without washing during the whole process, thus simplifying experimental steps, and improving detection efficiency.

Abstract: A nucleic acid amplification blocker for detecting a low-abundance mutation sequence and an application thereof in detecting a low-abundance mutation sequence are provided. The nucleic acid amplification blocker is an oligonucleotide modified by locked nucleic acid (LNA), and the matching region of the nucleic acid amplification blocker is located between amplified sequences. The nucleic acid amplification blocker is completely complementary to wild-type gene sequence, and contains at least one mismatch with mutant sequence. The nucleic acid amplification blocker has a great difference in affinity with mutant nucleic acid sequence/wild-type nucleic acid sequence, so as to achieve the purpose of highly selective amplification/enrichment of mutant sequence in samples. The nucleic acid amplification blocker has more significant detection effect on deletion mutation and insertion mutation.

Abstract: Disclosed is a carrier particle, comprising a sphere and a sub-sphere on the surface of the sphere; the sphere and/or at least one sub-sphere comprise at least one type of quantum dots. Also disclosed is a method for preparing the carrier particle, mainly by connecting at least one nanosphere to a microsphere, and the microsphere and/or the nanosphere are loaded with at least one type of quantum dots.

Abstract: Disclosed is a carrier particle, comprising a sphere and a sub-sphere on the surface of the sphere; the sphere and/or at least one sub-sphere comprise at least one type of quantum dots. Also disclosed is a method for preparing the carrier particle, mainly by connecting at least one nanosphere to a microsphere, and the microsphere and/or the nanosphere are loaded with at least one type of quantum dots.

Abstract: Polymerization process for preparing mono-disperse organic/inorganic nanocomposite microspheres in the field of nano technology is disclosed. The process comprises preparing of two different miniemulsion systems of A with inorganic nanoparticles and B with polymeric monomers in advance; adding water-soluble initiator into miniemulsion A, stirring till complete dissolution finally, heating the mixed system to a given temperature for isothermal reaction. The grain size of the as-prepared superparamagnetic Fe3O4/polystyrene using to present invention is homogeneous and controllable, and the magnetic particles content is controllable.

Abstract: Polymerization process for preparing mono-disperse organic/inorganic nanocomposite microspheres in the field of nano technology is disclosed. The process comprises preparing of two different miniemulsion systems of A with inorganic nanoparticles and B with polymeric monomers in advance; adding water-soluble initiator into miniemulsion A, stirring till complete dissolution finally, heating the mixed system to a given temperature for isothermal reaction. The grain size of the as-prepared superparamagnetic Fe3O4/polystyrene using to present invention is homogeneous and controllable, and the magnetic particles content is controllable.