Abstract: The invention features compositions and methods for delivering small interfering (siRNAs), e.g., shRNAs, to host cells using non-pathogenic strains of Salmonella bacteria containing nucleic acid expression constructs encoding shRNAs. In this process, shRNA expressed by the Salmonella silences or knocks down genes of interest (target genes) inside target cells. The nucleic acid expression constructs of the invention include an RNA polymerase (e.g., a T7 polymerase), an RNA polymerase promoter (e.g., a T7 polymerase promoter), and an RNA polymerase terminator (e.g., a T7 polymerase terminator). The Salmonella bacteria can also include, on the same or different nucleic acid construct, an endosomal release factor (e.g., HlyA).

Abstract: The invention features compositions and methods for delivering small interfering (siRNAs), e.g., shRNAs, to host cells using non-pathogenic strains of Salmonella bacteria containing nucleic acid expression constructs encoding shRNAs. In this process, shRNA expressed by the Salmonella silences or knocks down genes of interest (target genes) inside target cells. The nucleic acid expression constructs of the invention include an RNA polymerase (e.g., a T7 polymerase), an RNA polymerase promoter (e.g., a T7 polymerase promoter), and an RNA polymerase terminator (e.g., a T7 polymerase terminator). The Salmonella bacteria can also include, on the same or different nucleic acid construct, an endosomal release factor (e.g., HlyA).

Abstract: The present invention provides the cloning of intron-containing Amaranthus caudatus agglutinin (ACA) gene from Amaranthus caudatus and the coding region gene sequence encoding the ACA protein. The said gene sequence of the ACA gene coding region was ligated with highly efficient and stable microbial expression vectors to produce the ACA protein using microbe largely. High expression of the ACA gene can make the transgenic plants have antiaphid activity and improve the nutrition quality thereof. The ACA gene has potential application value in plant anti-insect gene engineering and quality improvement.

Abstract: The present invention provides the cloning of intron-containing Amaranthus caudatus agglutinin (ACA) gene from Amaranthus caudatus and the coding region gene sequence encoding the ACA protein. The said gene sequence of the ACA gene coding region was ligated with highly efficient and stable microbial expression vectors to produce the ACA protein using microbe largely. High expression of the ACA gene can make the transgenic plants have antiaphid activity and improve the nutrition quality thereof. The ACA gene has potential application value in plant anti-insect gene engineering and quality improvement.