Abstract: Disclosed herein are high Tm RNA nanostructures that can be composed of one or more modules or motifs to build RNA nanostructures with or without layers. The RNA nanostructures can have a core domain and three or more double-stranded arms and formulations thereof to conjugate high copy numbers of therapeutics, pH responsive or enzyme cleavable drug cargo. Also described herein is a design strategy for generation of synthetic RNA oligonucleotides that can self assemble into highly thermostable RNA structures. Also described herein are uses of the RNA nanostructures described herein.

Abstract: Disclosed herein is a system for detecting the presence of a target molecule, such as a target single stranded nucleic acid or polypeptide, in a fluid sample. The system can involve a lateral flow device that includes a porous lateral flow test strip, a solvent reservoir, and an immobilized oligonucleotide that selectively binds to a first portion of the target molecule affixed to the lateral flow test strip at a detection spot or line. The system can further involve a marker oligonucleotide selectively binds a second portion of the target molecule conjugated to a detection reagent. The system can further involve a control oligonucleotide complementary to a portion of the marker oligonucleotide affixed to the lateral flow test strip at a control spot or line. The system can also involve a solvent configured to drag a sample through the lateral flow test strip by capillary forces.

Abstract: Disclosed herein are high Tm RNA nanostructures that can be composed of one or more modules or motifs to build RNA nanostructures with or without layers. The RNA nanostructures can have a core domain and three or more double-stranded arms and formulations thereof to conjugate high copy numbers of therapeutics, pH responsive or enzyme cleavable drug cargo. Also described herein is a design strategy for generation of synthetic RNA oligonucleotides that can self assemble into highly thermostable RNA structures. Also described herein are uses of the RNA nanostructures described herein.