Abstract: The present disclosure provides a kit and method for constructing a long-acting depression animal model. The present disclosure adopts Bacille Calmette-Guerin (BCG) (or Mycobacterium bovis (M bovis)) and low-dose pertussis toxin (PTX) for combined induction, which greatly reduces modeling cost. A mouse model constructed by the method of the present disclosure can clearly distinguish a time interval of pathological behaviors from a time interval of depression-like behaviors and has prominent face validity and predictive validity. The method of the present disclosure is simple and convenient and does not require a large number of tedious daily operations as the depression model construction by chronic unpredictable stress, chronic restraint stress, social defeat, mother-child separation, or the like. The mouse model of the present disclosure can provide a valuable long-term intervention window for research on antidepressant therapy.

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

Abstract: In some examples, a semiconductor package comprises an electrically conductive surface and a bond wire coupled to the electrically conductive surface. The bond wire includes a first stitch bond coupled to the electrically conductive surface, and a second stitch bond contiguous with the first stitch bond and coupled to the electrically conductive surface. The second stitch bond is partially, but not completely, overlapping with the first stitch bond.

Abstract: The present application discloses a cerebral perfusion state classification apparatus and method, a device, and a storage medium. The method includes: acquiring, by a transceiving module, cervical blood flow data from an ultrasound data collecting device; determining, by a processor, cerebral perfusion data corresponding to the cervical blood flow data based on the cervical blood flow data and a mapping relationship between the cervical blood flow data and the cerebral perfusion data, and classifying cerebral perfusion states of a plurality of brain regions based on blood perfusion characteristics of the plurality of brain regions in the cerebral perfusion data.

Abstract: The present invention pertains to methods for characterizing proteins in a sample using native capillary electrophoresis-mass spectrometry. The present invention pertains to methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample, detecting and/or discriminating between antibodies in an antibody mixture, and characterizing monospecific antibody side products in a bispecific antibody sample.

Abstract: An anti-coking equipment, a preparation method therefor, and the use thereof. The preparation method comprises: bringing a low-oxygen partial pressure gas into contact with an equipment for reaction to obtain an anti-coking equipment containing an oxide film on the inner surface, wherein the dew point of the low-oxygen partial pressure gas is -40° C. to 40° C. A dense and stable oxide film is formed on the inner surface of the equipment prepared by the method, which can inhibit or slow down the catalytic coking phenomenon, reduce the degree of equipment carburization, and prolong the service life of the equipment.

Abstract: A planetary gear train automatic limited slip differential may consist of a main differential, a planetary gear train differential controller, a left axle shaft, a right axle shaft, and a clutch. The planetary gear train differential controller may be composed of a first planetary gear train differential controller unit and a second planetary gear train differential controller unit. The first planetary gear train differential controller unit may be composed of a first planetary gear train and a first overrunning clutch connected to the first planetary gear train. The second planetary gear train differential controller unit may be composed of a second planetary gear train and a second overrunning clutch connected to the second planetary gear train.

Abstract: An apparatus for metal-ion removal includes a conduit including an inlet to receive a liquid and an outlet to discharge the liquid, a first porous electrode and a second porous electrode disposed in the conduit, and a power source configured to provide power to the first porous electrode and the second porous electrode. The first porous electrode and the second porous electrode are separated by a gap. The first porous electrode is extended in a first direction. A flow direction of the liquid in the conduit is not in parallel with the first direction.

Abstract: A planetary gear train automatic limited slip differential may consist of a main differential, a planetary gear train differential controller, a left axle shaft, a right axle shaft, and a clutch. The planetary gear train differential controller may be composed of a first planetary gear train differential controller unit and a second planetary gear train differential controller unit. The first planetary gear train differential controller unit may be composed of a first planetary gear train and a first overrunning clutch connected to the first planetary gear train. The second planetary gear train differential controller unit may be composed of a second planetary gear train and a second overrunning clutch connected to the second planetary gear train.

Abstract: The present invention pertains to methods for characterizing proteins in a sample using native capillary electrophoresis-mass spectrometry. The present invention pertains to methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample, detecting and/or discriminating between antibodies in an antibody mixture, and characterizing monospecific antibody side products in a bispecific antibody sample.

Abstract: Methods for identifying viral protein constituents and quantifying the relative abundance of such viral protein constituents in a sample of viral particles are disclosed. In embodiments, the methods include first-dimension chromatography to separate intact viral capsid components of the sample, online denaturation of the viral capsid components to produce intact viral proteins, second-dimension chromatography to separate the viral proteins, and mass spectrometry to determine the masses of the viral proteins and identify the viral protein constituents of the sample.

Abstract: Disclosed is a lycopene food colorant having a fresh-keeping function. The food colorant is prepared from the following raw materials in parts by weight: 1-3 parts of a lycopene crystal, 5-20 parts of an emulsifier, 0.5-5 parts of an antioxidant, 60-70 parts of glycerol, and 10-20 parts of water. The food colorant is prepared by mixing the raw materials and then emulsifying and grinding same. The granularity of the obtained lycopene colorant is moderate; the tone is suitable for coloring a minced fillet product; the lycopene colorant has high tinting strength, achieves a bright color, and has the effect of triggering myoglobin, so that the minced fillet product has a bright color and appears fresher; the lycopene colorant further has a fresh-keeping function and can kill some food microorganisms, so as to keep food fresh and prolong the shelf life.

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

Abstract: Methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. In certain aspects, methods and media improve protein titer, cell growth, and/or viable cell density.

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

Abstract: Disclosed is a lycopene food colorant having a fresh-keeping function. The food colorant is prepared from the following raw materials in parts by weight: 1-3 parts of a lycopene crystal, 5-20 parts of an emulsifier, 0.5-5 parts of an antioxidant, 60-70 parts of glycerol, and 10-20 parts of water. The food colorant is prepared by mixing the raw materials and then emulsifying and grinding same. The granularity of the obtained lycopene colorant is moderate; the tone is suitable for coloring a minced fillet product; the lycopene colorant has high tinting strength, achieves a bright color, and has the effect of triggering myoglobin, so that the minced fillet product has a bright color and appears fresher; the lycopene colorant further has a fresh-keeping function and can kill some food microorganisms, so as to keep food fresh and prolong the shelf life.

Abstract: The present invention provides a substrate having a unidirectional water transport property, the substrate comprised of a fluid permeable structure and including: an inner side surface; and an outer side surface having a higher absorbent capacity than the inner side surface, wherein the inner side surface has a hydrophobic surface layer extending continuously over at least one section thereof, the hydrophobic surface layer having a predetermined thickness which, in use, produces a substantial hydrophobic property to contacting water, whilst allowing for water contacting the inner side surface of the substrate to wick through the hydrophobic surface layer into the substrate; and wherein the substrate is respectively comprised of hydrophobic channels and hydrophilic channels which respectively extend between the inner side surface and the outer side surface.

Abstract: Methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample. The methods including: contacting a sample comprising one or more antibodies of interest with a protease to digest the sample into antibody fragments; separating antibody fragments by molecular weight and/or charge in one or more capillaries using capillary electrophoresis; eluting separated antibody fragments from the one or more capillaries; and determining the mass of the eluted antibody fragments by mass spec analysis, thereby detecting and/or discriminating between post-translational modification variants of the antibody of interest.