Patents by Inventor Huan-Yao Lei

Huan-Yao Lei has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9086409
    Abstract: The present invention is about a microfluidic chip for rapid detection of different target proteins and a method for using the same. The microfluidic chip utilizes antibody-conjugated magnetic beads to bind to the target proteins to form a magnetic complex, and then use the signal labeled-antibodies that can recognize said magnetic complex. Purifying said magnetic complex by the micro-magnetic field on biochip, and introducing said purified magnetic complex into the fluorescent detection area on the chip to detect the amount of the target protein in said purified complex immediately.
    Type: Grant
    Filed: March 9, 2010
    Date of Patent: July 21, 2015
    Assignee: NATIONAL CHENG KUNG UNIVERSITY
    Inventors: Gwo-Bin Lee, Huan-Yao Lei, Yu-Fang Lee, Kang-Yi Lien
  • Publication number: 20120171660
    Abstract: The present invention relates to methods for screening or purifying enteroviruses, a method for mass-producing enteroviruses, and a method for manufacturing an enterovirus vaccine. The method for screening enteroviruses in a sample comprises the following steps: (A) providing a sample and a carrier, wherein monosaccharides such as glucose or galactose are bound to the surface of the carrier, and the monosaccharides have binding affinity to enterovirus; (B) contacting the sample with the carrier; (C) removing components of the sample that do not bind to the carrier; (D) providing a detection unit and contacting the detection unit with the carrier, wherein the detection unit binds to the sample bound on the carrier; and (E) measuring a signal of the detection unit, wherein when the signal of the detection unit is detected, it represents that the enterovirus exists in the sample.
    Type: Application
    Filed: July 14, 2011
    Publication date: July 5, 2012
    Applicant: National Cheng Kung University
    Inventors: Huan-Yao LEI, Chia-Ming Liu
  • Publication number: 20110144031
    Abstract: The present invention relates to a pharmaceutical composition for inducing damages of endothelial cells, a pharmaceutical composition for treating a tumor, and a method for treating a tumor by using the same. In addition, the pharmaceutical compositions for inducing damages of endothelial cells comprises: an effective amount of Concanavalin A (Con A).
    Type: Application
    Filed: September 23, 2010
    Publication date: June 16, 2011
    Applicant: National Cheng Kung University
    Inventors: Huan-Yao Lei, Ming-Chen Yang
  • Publication number: 20100248258
    Abstract: The present invention is about a microfluidic chip for rapid detection of different target proteins and a method for using the same. The microfluidic chip utilizes antibody-conjugated magnetic beads to bind to the target proteins to form a magnetic complex, and then use the signal labeled-antibodies that can recognize said magnetic complex. Purifying said magnetic complex by the micro-magnetic field on biochip, and introducing said purified magnetic complex into the fluorescent detection area on the chip to detect the amount of the target protein in said purified complex immediately.
    Type: Application
    Filed: March 9, 2010
    Publication date: September 30, 2010
    Applicant: National Cheng Kung University
    Inventors: Gwo-Bin Lee, Huan-Yao Lei, Yu-Fang Lee, Kang-Yi Lien
  • Patent number: 7718775
    Abstract: The present invention provides a monoclonal antibody capable of neutralizing EV71 infection.
    Type: Grant
    Filed: April 12, 2006
    Date of Patent: May 18, 2010
    Assignee: ScinoPharm Taiwan, Ltd.
    Inventors: Jui-Hung Yen, Hung-Jung Wang, Huan Yao Lei, Chun-Keung Yu, Ya-Fang Wang
  • Publication number: 20060148681
    Abstract: The present invention provides a method for treating tumor and/or preventing tumor metastasis and relapse comprising administrating a subject with a therapeutically effective amount of lectin capable of binding to the tumor and having tumor cytotoxicity and/or lymphocyte activation activity.
    Type: Application
    Filed: December 29, 2005
    Publication date: July 6, 2006
    Inventors: Huan-Yao Lei, Chih-Peng Chang, Wei-Chun Cheng
  • Publication number: 20060110779
    Abstract: The present invention provides a method for detecting lectin-like substances, which comprises the steps of (a) providing a first carbohydrate molecule and a test target, wherein the first carbohydrate molecule specifically binds to the lectin-like substance to be detected; (b) contacting the test target with the first carbohydrate molecule so that the lectin-like substance to be detected in the test target, if any, forms a complex with the first carbohydrate molecule; (c) removing the test target which does not form a complex with the first carbohydrate; (d) providing a detection unit comprising a second carbohydrate molecule and a reporter linked to the second carbohydrate molecule, wherein the second carbohydrate molecule is capable of specifically binding to the lectin-like substance to be detected; (e) contacting the detection unit of Step (d) with the complex formed in Step (b); (f) removing the detection unit which does not bind to the complex formed in Step (b); and (g) detecting the presence or absenc
    Type: Application
    Filed: November 21, 2005
    Publication date: May 25, 2006
    Inventors: Huan-Yao Lei, Chih-Peng Chang
  • Publication number: 20040170963
    Abstract: An RNA molecule for inhibiting expression of a gene of a virus. Also disclosed are a DNA vector encoding the RNA molecule and a pharmaceutical composition containing the RNA molecule or vector and a pharmaceutically acceptable carrier, as well as methods for inhibiting expression of a gene of a virus, replication of the virus, and infection with the virus.
    Type: Application
    Filed: December 3, 2003
    Publication date: September 2, 2004
    Inventors: Ih-Jen Su, Wen-Tsan Chang, Huan-Yao Lei, Tsung-Lin Cheng, Wen-Wei Chang, Ming-Derg Lai, Wen-Ya Huang
  • Patent number: 6777445
    Abstract: The present invention relates to a method for treating a disease, comprising administering a therapeutically effective amount of a pharmaceutical composition comprising a fullerene. The diseases treated comprise bacterial and viral diseases such as those cause by Gram positive and Gram negative bacteria, Dengue 2 virus and viral encephalitis. The fullerene is administered in vivo in an amount of about 0.001 to about 100 mg/kg of body weight of the subject.
    Type: Grant
    Filed: October 17, 2001
    Date of Patent: August 17, 2004
    Assignee: National Health Research Institute
    Inventors: Huan-Yao Lei, Chen-Kung Chou, Tien-Yau Luh
  • Publication number: 20020098180
    Abstract: The present invention relates to a method for treating a disease, comprising administering a therapeutically effective amount of a pharmaceutical composition comprising a fullerene. The diseases treated comprise bacterial and viral diseases such as those cause by Gram positive and Gram negative bacteria, Dengue 2 virus and viral encephalitis. The fullerene is administered in vivo in an amount of about 0.001 to about 100 mg/kg of body weight of the subject.
    Type: Application
    Filed: October 17, 2001
    Publication date: July 25, 2002
    Inventors: Huan-Yao Lei, Chen-Kung Chou, Tien-Yau Luh