Abstract: This disclosure provides automatic compensation methods, corresponding devices and a corresponding flow cytometer. The automatic compensation method includes: determining a base cell population and a reference cell population in the cell populations according to positions of the cell populations in a dot plot that needs to be compensated, where the base cell population is a double negative cell population and the reference cell population is a single positive cell population adjacent to the base cell population in a compensating direction (S10); calculating automatically a compensation value through a progressive approximation algorithm according to a position of the base cell population, and updating the dot plot with the compensation value, so that the position difference between the reference cell population and the base cell population both in the dot plot in the compensating direction is within a predetermined range (S16).

Abstract: This disclosure provides automatic compensation methods, corresponding devices and a corresponding flow cytometer. The automatic compensation method includes: determining a base cell population and a reference cell population in the cell populations according to positions of the cell populations in a dot plot that needs to be compensated, where the base cell population is a double negative cell population and the reference cell population is a single positive cell population adjacent to the base cell population in a compensating direction (S10); calculating automatically a compensation value through a progressive approximation algorithm according to a position of the base cell population, and updating the dot plot with the compensation value, so that the position difference between the reference cell population and the base cell population both in the dot plot in the compensating direction is within a predetermined range (S16).

Abstract: A flow cytometer and a fluid system are provided. The fluid system comprises a flow cell, a sample providing unit, a waste container, a sheath container, a negative pressure source, a quantitative unit and a sample flow monitoring unit, the negative pressure source, the waste container and the flow cell are connected, a negative pressure source, which provides a negative pressure relative to the sample providing unit for the flow cell so that the sample providing unit causes the sample to flow into the flow cell under the negative pressure, a sample flow monitoring unit monitors a flow of the sample fluid transported from the sample providing unit to the flow cell and outputs a feedback signal reflecting flow changes of the sample fluid in real-time; wherein the controller receives the feedback signal and controls the quantitative unit to adjust a flow of the sheath fluid according to the feedback signal.

Abstract: A flow cytometer and a fluid system are provided. The fluid system comprises a flow cell, a sample providing unit, a waste container, a sheath container, a negative pressure source, a quantitative unit and a sample flow monitoring unit, the negative pressure source, the waste container and the flow cell are connected, a negative pressure source, which provides a negative pressure relative to the sample providing unit for the flow cell so that the sample providing unit causes the sample to flow into the flow cell under the negative pressure, a sample flow monitoring unit monitors a flow of the sample fluid transported from the sample providing unit to the flow cell and outputs a feedback signal reflecting flow changes of the sample fluid in real-time; wherein the controller receives the feedback signal and controls the quantitative unit to adjust a flow of the sheath fluid according to the feedback signal.