Patents by Inventor Hyohak Song
Hyohak Song has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9428774Abstract: The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid.Type: GrantFiled: October 3, 2015Date of Patent: August 30, 2016Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup Lee, Sung Won Lim, Hyohak Song
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Publication number: 20160017383Abstract: The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid.Type: ApplicationFiled: October 3, 2015Publication date: January 21, 2016Applicant: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Sung Won Lim, Hyohak Song
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Patent number: 9217138Abstract: The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid.Type: GrantFiled: January 25, 2009Date of Patent: December 22, 2015Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup Lee, Sung Won Lim, Hyohak Song
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Patent number: 8877482Abstract: The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.Type: GrantFiled: January 15, 2008Date of Patent: November 4, 2014Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Ji Mahn Kim, Jeong Wook Lee, Hyohak Song, Sol Choi
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Patent number: 8765446Abstract: The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms.Type: GrantFiled: September 21, 2010Date of Patent: July 1, 2014Assignees: Korea Advanced institute of Science and Technology, Biofuelchem Co., GS Caltex CorporationInventors: Sang Yup Lee, Yu-Sin Jang, Joung Min Lee, Jung Ae Im, Hyohak Song
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Publication number: 20120301936Abstract: The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms.Type: ApplicationFiled: September 21, 2010Publication date: November 29, 2012Applicants: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, GS CALTEX CORPORATION, BIOFUELCHEM CO.Inventors: Sang Yup Lee, Yu-Sin Jang, Joung Min Lee, Jung Ae Im, Hyohak Song
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Publication number: 20110269183Abstract: The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding ?-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.Type: ApplicationFiled: December 18, 2008Publication date: November 3, 2011Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup Lee, Jeong Wook Lee, Hyohak Song, Ji Mahn Kim, Sol Choi, Jin Hwan Park
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Patent number: 7803587Abstract: A method for developing a culture medium using genome information and in silico analysis. In one implementation, the method involves developing a minimal synthetic medium, including the steps of constructing a metabolic network using genome information of prokaryotic cell or eukaryotic cell, selecting components of the minimal synthetic medium removing any one among external metabolites from the constructed metabolic network and conducting metabolic flux analysis using in silico simulation, and determining a final culture medium by actual culture.Type: GrantFiled: May 2, 2007Date of Patent: September 28, 2010Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Ho Nam Chang, Hyohak Song, Tae Yong Kim, Bo-Kyung Choi
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Publication number: 20100159544Abstract: The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.Type: ApplicationFiled: January 15, 2008Publication date: June 24, 2010Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup Lee, Ji Mahn Kim, Jeong Wook Lee, Hyohak Song, Sol Choi
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Publication number: 20090203095Abstract: The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid.Type: ApplicationFiled: January 25, 2009Publication date: August 13, 2009Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup LEE, Sung Won LIM, Hyohak SONG
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Patent number: 7572615Abstract: Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.Type: GrantFiled: April 20, 2007Date of Patent: August 11, 2009Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Hyohak Song, Yu Sin Jang, Sung Won Lim
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Patent number: 7470770Abstract: A nucleotide sequence encoding a malic enzyme and a method for preparing succinic acid using the same, more particularly, a maeB nucleotide sequence encoding a malic enzyme B having the activity of converting pyruvic acid or pyruvate to malic acid or malate, or vice versa, a recombinant vector containing the gene, a microorganism transformed with the recombinant vector, and a method for preparing succinic acid using the transformed microorganism.Type: GrantFiled: September 16, 2005Date of Patent: December 30, 2008Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Hyohak Song, Yu Sin Jang, Jeong Wook Lee
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Publication number: 20080020436Abstract: Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.Type: ApplicationFiled: April 20, 2007Publication date: January 24, 2008Inventors: Sang Lee, Hyohak Song, Yu Jang, Sung Lim
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Publication number: 20080003661Abstract: A method for developing a culture medium using genome information and in silico anlaylsis. In one implementation, the method involves developing a minimal synthetic medium, including the steps of constructing a metabolic network using genome information of prokaryotic cell or eukaryotic cell, selecting components of the minimal synthetic medium removing any one among external metabolites from the constructed metabolic network and conducting metabolic flux analysis using in silico simulation, and determining a final culture medium by actual culture.Type: ApplicationFiled: May 2, 2007Publication date: January 3, 2008Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Lee, Ho Chang, Hyohak Song, Tae Kim, Bo-Kyung Choi
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Patent number: 7241594Abstract: Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.Type: GrantFiled: September 16, 2005Date of Patent: July 10, 2007Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Hyohak Song, Yu Sin Jang, Sung Won Lim
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Publication number: 20070042476Abstract: A nucleotide sequence encoding a malic enzyme and a method for preparing succinic acid using the same, more particularly, a maeB nucleotide sequence encoding a malic enzyme B having the activity of converting pyruvic acid or pyruvate to malic acid or malate, or vice versa, a recombinant vector containing the gene, a microorganism transformed with the recombinant vector, and a method for preparing succinic acid using the transformed microorganism.Type: ApplicationFiled: September 16, 2005Publication date: February 22, 2007Inventors: Sang Lee, Hyohak Song, Yu Jang, Jeong Lee
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Publication number: 20070042481Abstract: Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.Type: ApplicationFiled: September 16, 2005Publication date: February 22, 2007Inventors: Sang Lee, Hyohak Song, Yu Jang, Sung Lim
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Publication number: 20070042477Abstract: A nucleotide sequence encoding a fumarate hydratase C and a method for preparing succinic acid using the same, more particularly, a fumarate hydratase C having the activity of converting malate to fumarate, a fumC nucleotide sequence encoding the fumarate hydratase C, a recombinant vector containing the nucleotide sequence, a microorganism transformed with the recombinant vector, and a method for preparing succinic acid using the transformed microorganism.Type: ApplicationFiled: September 16, 2005Publication date: February 22, 2007Inventors: Sang Lee, Hyohak Song, Yu Jang, Ji Kim