Abstract: An electrocardiographic (ECG) device and fabrication methods of the ECG device are provided for sensing cardiac activities in a test subject. The ECG device includes a plurality of electrodes and a plurality of bridges connecting adjacent electrodes of the plurality of electrodes. The electrodes each has a structure including a first layer and a third layer disposed above the third layer, a second layer of liquid metal disposed between the first and third layers, and a fourth layer of conductive material disposed on the third layer, and the second layer of liquid metal is electrically connected with the fourth layer of conductive material. Each electrode may include a metal film connecting the second layer of liquid metal with the fourth layer of conductive material. The first and third layers are formed with stretchable material, making the ECG device skin-adherent, highly stretchable, and conformal.

Abstract: The subject invention relates to a contamination-free method that utilizes CRISPR/Cas enzyme specific sequence recognition towards the loop region in the LAMP amplicons to accomplish one-pot detection for rapid visual readout diagnostics of the presence or absence of target nucleic acid sequences.

Abstract: The subject invention pertains to composition and methods of using said composition as an in vitro biosensor of small molecules in biological and/or environmental samples using enzyme-assisted nucleic acid reactions. The methods and compositions can be used to sense and/or transduce the signal of a sensing event mediated by allosteric proteins, endonucleases and nucleic acid reactions. This invention allows the rapid development and setup of one-pot assays to provide results in minutes. The methods and compositions may be used to generate an electrochemical, fluorescent, colorimetric, and/or luminescent output and the methods can be performed in different modalities, including a solution-based or paper-based assay.

Abstract: The subject invention pertains to the rapid amplification and real-time monitoring of nucleic acid sequences at a constant temperature. Specifically, fluorescent or electroactive-labeled loop probes and primers are designed to be specific to identified regions of a target nucleic acid sequences and subsequently amplify and permit the identification of the presence of the target sequence. In this method, a DNA polymerase with no 5? to 3? exonuclease activity is added to extend the primers and probes, while a nicking endonuclease is added to specifically identify the amplified nucleic acid product and cleave the label from the loop probe-extended DNA duplex.

Abstract: The subject invention pertains to methods for the analysis of pooled samples without the need of retesting through the use of oligonucleotide hybridization and target-specific amplification reactions. Specifically, a series of identifier oligonucleotides with different sequence compositions, each corresponding to a distinct sample, are combined into the target template of interest through nucleic acid synthesis. The aforementioned products are pooled together, and the pooled samples are amplified and detected using the probe-based hybridization assay or a size separation module to identify if any of the pool of samples test positive, as well as simultaneously identifying which sample is positive for the targeted sequence.

Abstract: The subject invention pertains to composition and methods of using said composition as an in vitro biosensor of small molecules in biological and/or environmental samples using enzyme-assisted nucleic acid reactions. The methods and compositions can be used to sense and/or transduce the signal of a sensing event mediated by allosteric proteins, endonucleases and nucleic acid reactions. This invention allows the rapid development and setup of one-pot assays to provide results in minutes. The methods and compositions may be used to generate an electrochemical, fluorescent, colorimetric, and/or luminescent output and the methods can be performed in different modalities, including a solution-based or paper-based assay.

Abstract: The subject invention pertains to the rapid amplification and real-time monitoring of nucleic acid sequences at a constant temperature. Specifically, fluorescent or electroactive-labeled loop probes and primers are designed to be specific to identified regions of a target nucleic acid sequences and subsequently amplify and permit the identification of the presence of the target sequence. In this method, a DNA polymerase with no 5? to 3? exonuclease activity is added to extend the primers and probes, while a nicking endonuclease is added to specifically identify the amplified nucleic acid product and cleave the label from the loop probe-extended DNA duplex.

Abstract: The present invention provides methods for determining a haplotype or a diplotype in a genetic sample. The method comprises the steps of contacting a probe-complex with the genetic sample, wherein the probe complex comprises at least two probes, hybridizing at least two probes to a polynucleotide sequence, each of which is specific to one of two or more genetic variants, determining the presence or absence of at least one genetic variant by detecting a signal emitted from at least one probe, wherein detection of said signal is indicative of the the presence of a genetic variant, removing or displacing at least one of said probes from said sample, and detecting a change in the signal to determine the haplotype or a diplotype in the genetic sample. Kits for use in the method of the invention are also provided.

Abstract: A method for electrochemically or electrically detecting nucleic acids, utilizes electrochemically active or electrically conductive reporter materials. An electric voltage is applied and electric signals are measured to the electrodes that are suitable for detecting or quantifying the nucleic acid(s) in a sample. This technique is suitable for point-of-use applications, e.g. detecting bioanalytes in remote locations. A microchip, device, kit used adapted to be used for this method is also disclosed.

Abstract: A method for real-time electrochemical monitoring of PCR amplicons using a hydrolysis probe that is labeled with electro-active indicators and a microchip for implementing the method. The method provided is simpler and has higher specificity compared with the prior art. The electrochemical signal measured during the PCR process can be used to determine the initial amount of the target DNA. This technique can be applied in detection and quantification of nucleic acids, especially for point-of-use applications such as on-site nucleic acid-based bio-analysis.

Abstract: A method and device for real time electrochemically or electrically monitoring and detecting nucleic acid amplification products, i.e. after each polymerase chain reaction cycle, utilizes electrochemically active or electrically conductive reporter materials. An electric voltage is applied and electric signals are measured during a PCR amplification process to the electrodes that is suitable for quantifying the amplified products of a sample's nucleic acid(s) produced. This technique is suitable for point-of-use applications, e.g. detecting bioanalytes in remote locations.

Abstract: A method for electrochemically or electrically detecting nucleic acids, utilizes electrochemically active or electrically conductive reporter materials. An electric voltage is applied and electric signals are measured to the electrodes that are suitable for detecting or quantifying the nucleic acid(s) in a sample. This technique is suitable for point-of-use applications, e.g. detecting bioanalytes in remote locations. A microchip, device, kit used adapted to be used for this method is also disclosed.

Abstract: A method for electrochemically or electrically detecting nucleic acids, utilizes electrochemically active or electrically conductive reporter materials. An electric voltage is applied and electric signals are measured to the electrodes that are suitable for detecting or quantifying the nucleic acid(s) in a sample. This technique is suitable for point-of-use applications, e.g. detecting bioanalytes in remote locations. A microchip, device, kit used adapted to be used for this method is also disclosed.

Abstract: Provided herein is a method for manipulating the surface density of functional molecules conjugated to nanoparticles, which method including incubating nanoparticles with nucleotides to form nucleotide-coated nanoparticles, adjusting buffer and salt concentration of the conjugation media, adding thiolated molecules in the conjugation media to incubate with the nucleotie-coated nanoparticles, and adding thiolated oligo(ethylene glycol) in the conjugation media to cease the conjugation process of thiolated molecules to nanoparticles. The method is simple, efficient and cost effective, and the surface density of functional molecules can be quickly manipulated in a wide range for various applications, such as biosensing, molecular diagnostics, nanomedicine, and nano-assembly.

Abstract: A method and device for real time electrochemically or electrically monitoring and detecting nucleic acid amplification products, i.e. after each polymerase chain reaction cycle, utilizes electrochemically active or electrically conductive reporter materials. An electric voltage is applied and electric signals are measured during a PCR amplification process to the electrodes that is suitable for quantifying the amplified products of a sample's nucleic acid(s) produced. This technique is suitable for point-of-use applications, e.g. detecting bioanalytes in remote locations.

Abstract: A fuel cell has at least one electrode having channels for delivering reactants, products, or both. The electrode is an anode or cathode of the fuel cell, or both. The electrode can serve as both a liquid diffusion layer and a flow field plate, thus replacing the traditional elements of carbon paper, cloth diffusion layer, and anode current collector. In some aspects, the fuel cell uses methanol, and the electrode is formed from flexible graphite. The electrode can have a structure sufficient to permit methanol diffusion while preventing methanol crossover. The electrode can also improve volumetric power density and eliminate contact resistance typically present between a conventional flow field plate and conventional diffusion electrode layer.