Abstract: The present disclosure relates, in some embodiments, to a method of reducing levels of Candidatus Liberibacter solanacearum (Lso) in a potato, the method including (a) transforming the potato with an expression vector to generate a transformed potato, where the expression vector may include in a 5? to 3? direction: an expression control sequence; an exogenous nucleic acid operably linked to the expression control sequence; and a 3? termination sequence operably linked to the exogenous nucleic acid; and (b) cultivating the transformed potato in conditions suitable for expression of the exogenous nucleic acid. According to some embodiments an exogenous nucleic acid may include a nucleic acid sequence having at least 98% identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 4 and SEQ ID NO: 6.

Abstract: The present disclosure relates, according to some embodiments, to citrus shoot regeneration compositions, methods, and systems. For example, citrus shoot regeneration compositions (e.g., culture media) may comprise one or more non-ionic surfactants. Methods may comprise preparing and using these compositions in some embodiments. Regeneration systems may comprise, in some embodiments, one or more of these compositions and/or one or more citrus explants.

Abstract: The present disclosure relates, in some embodiments, to potato transformation compositions, systems, methods, microorganisms, and plants (e.g., one or more potato chipping varieties). In some embodiments, a method of transforming and/or transfecting a plant (e.g., ‘Atlantic’ potato) may comprise (a) growing an ‘Atlantic’ potato plant (e.g., from a tuber) for from about 3 weeks to about 4 weeks, (b) removing one or more leaf sections (e.g., each section from about 0.5 cm to about 1 cm in its longest dimension) from the plant, (c) cultivating the one or more sections on a callus induction medium comprising zeatin for about 2 days, and/or (d) contacting the one or more sections with Agrobacterium comprising the exogenous nucleic acids under conditions that permit transfer of the exogenous nucleic acid to the one or more sections to produce at least one transformed and/or transfected plant cell.