Abstract: The present invention provides an improved integrated system for biological marker identification. The system uses Microvolume Laser Scanning Microscopy (MLSC) in order to measure patterns of expression of biological markers in biological fluids. The system includes improved instrumentation for performing MLSC, and also includes improved particle detection and analysis methods. The system further comprises an informatics architecture for the analysis of data obtained from the MLSC in tandem with other medical information.

Abstract: A method and apparatus for improving reservoir communication includes, in one arrangement, causing creation of tunnels in surrounding formation of a well interval, and applying treatment fluid to the tunnels. A local transient underbalance condition is created in the well interval after creation of the tunnels in the formation and application of the treatment fluids.

Abstract: A system for controlling a dynamic pressure transient in a well during operations to provide fluid communication between the wellbore and adjacent formation. A method includes determining the characteristics of an adjacent formation, selecting a perforating tool for increasing fluid communication, determining a dynamic pressure transient to enhance the quality of the fluid communication and prevent damage, selecting a wellbore fluid to achieve the desired pressure transient, and performing the fluid communication process based on the selected parameters.

Abstract: Methods and instrumentation for performing charge coupled device (CCD)-based confocal spectroscopy with a laser spot array are provided. The methods and instruments of the invention are useful in any spectroscopic application, including, but not limited to, microscopy and microvolume laser scanning cytometry (MLSC).

Abstract: The present invention provides an improved integrated system for biological marker identification. The system uses Microvolume Laser Scanning Microscopy (MLSC) in order to measure patterns of expression of biological markers in biological fluids. The system includes improved instrumentation for performing MLSC, and also includes improved particle detection and analysis methods. The system further comprises an informatics architecture for the analysis of data obtained from the MLSC in tandem with other medical information.

Abstract: Methods and instrumentation for performing charge coupled device (CCD)-based confocal spectroscopy with a laser spot array are provided. The methods and instruments of the invention are useful in any spectroscopic application, including, but not limited to, microscopy and microvolume laser scanning cytometry (MLSC).

Abstract: A method and apparatus for improving reservoir communication includes, in one arrangement, use of one or more chambers to create an underbalance condition and/or a fluid surge in the wellbore. In another arrangement, a tool string comprises a packer, a circulating valve, and an atmospheric chamber, in which the circulating valve, when open, is adapted to vent a lower wellbore region below the packer when the packer is set, and the atmospheric chamber is capable of being operated to create an underbalance condition below the packer. In yet another arrangement, an apparatus comprises a subsea wellhead equipment including a blow-out preventer, choke line filled with a low density fluid, and a kill line filled with a heavy fluid. The choke line is adapted to be opened to create an underbalance condition in the wellbore.

Abstract: A method and apparatus for improving reservoir communication includes, in one arrangement, use of one or more chambers to create an underbalance condition and/or a fluid surge in the wellbore. In another arrangement, a tool string comprises a packer, a circulating valve, and an atmospheric chamber, in which the circulating valve, when open, is adapted to vent a lower wellbore region below the packer when the packer is set, and the atmospheric chamber is capable of being operated to create an underbalance condition below the packer. In yet another arrangement, an apparatus comprises a subsea wellhead equipment including a blow-out preventer, choke line filled with a low density fluid, and a kill line filled with a heavy fluid. The choke line is adapted to be opened to create an underbalance condition in the wellbore.

Abstract: A method and apparatus for imaging a sample are provided. An electromagnetic radiation source generates excitation radiation which is sized by excitation optics to a line. The line is directed at a sample resting on a support and excites a plurality of regions on the sample. Collection optics collect response radiation reflected from the sample I and image the reflected radiation. A detector senses the reflected radiation and is positioned to permit discrimination between radiation reflected from a certain focal plane in the sample and certain other planes within the sample.

Abstract: Labeled targets on a support synthesized with polymer sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, can be detected by exposing selected regions of sample 1500 to radiation from a source 1100 and detecting the emission therefrom, and repeating the steps of exposition and detection until the sample is completely examined.

Abstract: Labeled targets on a support synthesized with polymer sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, can be detected by exposing selected regions of sample 1500 to radiation from a source 1100 and detecting the emission therefrom, and repeating the steps of exposition and detection until the sample is completely examined.

Abstract: The present invention provides methods and systems for detecting a labeled marker on a sample located on a support. The imaging system comprises a body for immobilizing the support, an excitation radiation source and excitation optics to generate and direct the excitation radiation at the sample. In response, labeled material on the sample emits radiation which has a wavelength that is different from the excitation wavelength, which radiation is collected by collection optics and imaged onto a detector which generates an image of the sample.