Abstract: A process for the linear synthesis of a gram-positive class II bacteriocin or a variant thereof is disclosed herein. The process comprises the stepwise addition of selected amino acids to a solid support; pseudoproline positioning and reopening; and cleavage of the gram-positive class II bacteriocin or the variant thereof from the solid support to provide a linear gram-positive class II bacteriocin or variant thereof; and in situ disulfide bond formation. Various applications and uses of the synthetic bacteriocins are also disclosed. The synthetic process can also be used to synthesize variants of bacteriocins by the selective substitution of one or more amino acids and/or additions and/or deletions of selected amino acids.

Abstract: A process for the linear synthesis of a gram-positive class II bacteriocin or a variant thereof is disclosed herein. The process comprises the stepwise addition of selected amino acids to a solid support; pseudoproline positioning and reopening; and cleavage of the gram-positive class II bacteriocin or the variant thereof from the solid support to provide a linear gram-positive class II bacteriocin or variant thereof; and in situ disulfide bond formation. Various applications and uses of the synthetic bacteriocins are also disclosed. The synthetic process can also be used to synthesize variants of bacteriocins by the selective substitution of one or more amino acids and/or additions and/or deletions of selected amino acids.

Abstract: A process for the linear synthesis of a gram-positive class II bacteriocin or a variant thereof is disclosed herein. The process comprises the stepwise addition of selected amino acids to a solid support; pseudoproline positioning and reopening; and cleavage of the gram-positive class II bacteriocin or the variant thereof from the solid support to provide a linear gram-positive class II bacteriocin or variant thereof; and in situ disulfide bond formation. Various applications and uses of the synthetic bacteriocins are also disclosed. The synthetic process can also be used to synthesize variants of bacteriocins by the selective substitution of one or more amino acids and/or additions and/or deletions of selected amino acids.

Abstract: The present invention relates to an antimicrobial composition having activity against Gram-positive and Gram-negative bacteria. The composition is a) a composition comprising at least two organic acids selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid; b) a composition comprising at least two bacteriocin selected from the group consisting of pediocin, Nisin and reuterin; c) a composition comprising pediocin and at least two organic acids selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid; or d) a composition comprising reuterin and at least one organic acid selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid. The present invention also relates to the use of said compositions for sanitizing and/or disinfecting surfaces and method thereof.

Abstract: The present invention relates to an antimicrobial composition having activity against Gram-positive and Gram-negative bacteria. The composition is a) a composition comprising at least two organic acids selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid; b) a composition comprising at least two bacteriocin selected from the group consisting of pediocin, Nisin and reuterin; c) a composition comprising pediocin and at least two organic acids selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid; or d) a composition comprising reuterin and at least one organic acid selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid. The present invention also relates to the use of said compositions for sanitizing and/or disinfecting surfaces and method thereof.

Abstract: The present invention relates to an antimicrobial composition having activity against Gram-positive and Gram-negative bacteria. The composition is a) a composition comprising at least two organic acids selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid; b) a composition comprising at least two bacteriocin selected from the group consisting of pediocin, Nisin and reuterin; c) a composition comprising pediocin and at least two organic acids selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid; or d) a composition comprising reuterin and at least one organic acid selected from the group consisting of lactic acid, acetic acid, benzoic acid and citric acid. The present invention also relates to the use of said compositions for sanitizing and/or disinfecting surfaces and method thereof.

Abstract: The present invention relates to novel maple product compositions. In particular, the present invention relates to new compositions comprising maple sap, maple concentrate, maple syrup or diluted maple syrup, and probiotics, prebiotics or combinations thereof. The present invention also discloses methods for the production of maple based products, including functional drinks, comprising a composition comprising maple sap, maple concentrate, maple syrup or dilutated maple syrup, and probiotics, prebiotics or combinations thereof, as a vehicle for the delivery of probiotics and prebiotics to the gastrointestinal tract.

Abstract: The present invention relates to novel maple product compositions. In particular, the present invention relates to new compositions comprising maple sap, maple concentrate, maple syrup or diluted maple syrup, and probiotics, prebiotics or combinations thereof. The present invention also discloses methods for the production of maple based products, including functional drinks, comprising a composition comprising maple sap, maple concentrate, maple syrup or dilutated maple syrup, and probiotics, prebiotics or combinations thereof, as a vehicle for the delivery of probiotics and prebiotics to the gastrointestinal tract.

Abstract: The present invention provide a new method for modulating the immune system of a subject in need thereof by administering a dairy-derived peptide to the subject, particularly a ?-lactoglubulin-derived peptide (?-Lg peptide). According to one aspect, the modulation of the immune system is the modulation of a Th1 response. According to another aspect, the modulation of the immune system is the modulation of a Th2 response.

Abstract: The present invention relates to a process using dynamic high-pressure for inactivation of food pathogens. Liquid food are treated by dynamic-high-pressure at 1 to 5 kbars with at least one recirculation depending on the needs. The pasteurization process is performed at relatively cold temperature ranging from 4° C. to 55° C.

Abstract: The present invention concerns an isolated bacteriocin, Divergicin M35, derived form Carnobacterium divergens M35. It also concerns a composition comprising an effective amount of Divergicin M35 and the use of that bacteriocin to kill or limit the proliferation of a microorganism.

Abstract: A method and a system is disclosed for the modulation of various characteristics of probiotic cells and for the production of probiotics having altered characteristics. Such altered characteristics include increased resistance to various stresses, drugs and chemical agents, as well as aggregation. Such a method and a system are useful for the production of probiotics with altered characteristics, which may for example provide health benefits when present in the gastrointestinal tract of an animal.

Abstract: The present invention concerns an isolated bacteriocin, Divergicin M35, derived form Carnobacterium divergens M35. It also concerns a composition comprising an effective amount of Divergicin M35 and the use of that bacteriocin to kill or limit the proliferation of a microorganism.

Abstract: A system utilizing cell immobilization in anaerobic continuous-flow cultures for modelling the gastrointestinal system is described. Microbial cells derived from flora, e.g. in fresh faecal samples, are used as the source of inocula for immobilisation in a mixed gel of gellan and xanthan. The beads produced are then introduced in a single or multi-stage chemostat fed with a nutrient media, and the composition and metabolic activities of the flora are monitored over time in reactors operated with conditions simulating the characteristics of different segments of the gastrointestinal tract. The conditions of this intestinal fermentation model are more akin to that for the gastrointestinal system, in which cells are naturally in the immobilized state, entrapped in fibrous particles or forming biofilms on the intestine epithelium. A use of such a system for studying various aspects of the gastrointestinal tract is also described.

Abstract: A system utilizing cell immobilization in anaerobic continuous-flow cultures for modelling the gastrointestinal system is described. Microbial cells derived from flora, e.g. in fresh faecal samples, are used as the source of inocula for immobilisation in a mixed gel of gellan and xanthan. The beads produced are then introduced in a single or multi-stage chemostat fed with a nutrient media, and the composition and metabolic activities of the flora are monitored over time in reactors operated with conditions simulating the characteristics of different segments of the gastrointestinal tract. The conditions of this intestinal fermentation model are more akin to that for the gastrointestinal system, in which cells are naturally in the immobilized state, entrapped in fibrous particles or forming biofilms on the intestine epithelium. A use of such a system for studying various aspects of the gastrointestinal tract is also described.

Abstract: A method and a system is disclosed for the modulation of various characteristics of probiotic cells and for the production of probiotics having altered characteristics. Such altered characteristics include increased resistance to various stresses, drugs and chemical agents, as well as aggregation. Such a method and a system are useful for the production of probiotics with altered characteristics, which may for example provide health benefits when present in the gastrointestinal tract of an animal.