Abstract: A release of the frozen solvent trapped inside a solidified gel occurs when the temperature increases, followed by a dissolving of a reactive substance present in a membrane that is located in direct physical contact with the gel, resulting in the reabsorption of the reactive substance dissolved in the solvent into the solid gel layer within a constant time. In this manner, the components generating color change come together within the solid gel layer initiating a reaction to change the color of the indicator layer. Thus, the increase in temperature above a melting point of the solid gel layer is enough to activate the intelligent label with an irreversible color change.

Abstract: A release of the frozen solvent trapped inside a solidified gel occurs when the temperature increases, followed by a dissolving of a reactive substance present in a membrane that is located in direct physical contact with the gel, resulting in the reabsorption of the reactive substance dissolved in the solvent into the solid gel layer within a constant time. In this manner, the components generating color change come together within the solid gel layer initiating a reaction to change the color of the indicator layer. Thus, the increase in temperature above a melting point of the solid gel layer is enough to activate the intelligent label with an irreversible color change.

Abstract: The present invention relates to methods for the detection of microorganisms. In one embodiment, the present invention provides methods for detecting live microorganisms in a culture by capturing and culting the microorganisms on para-tropic-coated paramagnetic beads. This technique is useful for any application in which it is necessary to monitor the biological contamination level, for example drinking water, recreational waters, food processing waters and medical laboratories. In one embodiment, the method for determining the concentration of viable microorganisms in a sample according to the invention further comprises an inducer reagent, wherein the inducer reagent includes an inducer compound that induces the activity of an enzyme unique to the microorganism of interest.