Abstract: The present invention relates to a dry grind ethanol process in which post-distillation backset is subjected to aerobic fermentation to remove components that are inhibitory to an ethanolagen, such as yeast, allowing the utilization of an increased amount of post-distillation backset during the initial preparation of starch-containing substrates for the dry grind ethanol process. Aerobic fermentation of the post-distillation backset allows substantially higher backset recycle, resulting in fresh water savings and increased sustainability.

Abstract: The present strains and methods relate to yeast cells that over-overproduce selected endogenous proteins having a high amino acid content of a selected amino acid. The yeast can be used in a conventional bioethanol production facility to produce alcohol along with increased amounts of a selected amino acid, resulting in increased quality and commercial value of fermentation products and co-products, such as animal feed ingredients.

Abstract: Described are strains and methods relating to genetically-engineered yeast cells that overproduce lysine in a tunable manner by altering feedback inhibition of the lysine synthetic pathway by way of the LYS20 and LYS21 homocitrate synthase polypeptides. The yeast can be used in a conventional bioethanol production facility to produce alcohol along with increased amounts of lysine, resulting in increased quality and commercial value of fermentation products and co-products, such as animal feed ingredients.

Abstract: Described are compositions and methods relating to yeast having a genetic mutation that results in decreased amounts of Cdc42 effector proteins, resulting in increased alcohol and lysine production. Such yeast is well-suited for use commercial alcohol production to increase yields and to increase the value of Such yeast is well-suited for use commercial alcohol production to increase yields and to increase the value of amino-acid-containing, fermentation-co-products.

Abstract: Disclosed are compositions and methods for improving the amino acid content of animal feed byproducts from a dry grind ethanol production facility by reducing the amount of residual reducing sugars that can participate in, e.g., the Maillard reaction.