Abstract: The present disclosure provides devices, systems, and methods related to protein bioelectronics. In particular, the present disclosure provides devices, systems, and methods for manipulating a protein-of-interest into a target position within two electrodes in order to generate a functional bioelectronic circuit. The present disclosure also provides devices, systems, and methods for selectively attracting and concentrating one or more target analytes to the protein-of-interest, which can be used to develop analytical platforms to detect and measure various characteristics of protein function.