Patents by Inventor Jacobus Thomas Pronk
Jacobus Thomas Pronk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150353942Abstract: The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.Type: ApplicationFiled: February 21, 2014Publication date: December 10, 2015Inventors: Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Victor Gabriel GUADALUPE MEDINA, Hendrik Wouter WISSELINK
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Patent number: 9034608Abstract: The invention relates to a polypeptide having a mutation at one or more position corresponding to T219 of SEQ ID NO: 55, wherein the polypeptide has at least 50% sequence identity with SEQ ID NO: 55, and wherein the polypeptide has permease activity.Type: GrantFiled: October 11, 2011Date of Patent: May 19, 2015Assignee: DSM IP ASSETS, B.V.Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paul Klaassen, Rene Marcel De Jong
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Patent number: 9023629Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: GrantFiled: November 29, 2012Date of Patent: May 5, 2015Assignee: DSM IP Assets B.V.Inventors: Hubertus Johannes Marie Op Den Camp, Harry Ramanoedj Harhangi, Christiaan Van Der Drift, Jacobus Thomas Pronk
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Publication number: 20150104543Abstract: Improved systems for the biological production of organic acids are described.Type: ApplicationFiled: September 25, 2014Publication date: April 16, 2015Inventors: Aaron Adriaan WINKLER, Abraham Frederik DE HULSTER, Johannes Pieter VAN DIJKEN, Jacobus Thomas PRONK, Joshua TRUEHEART, Kevin T. MADDEN, Carlos Gancedo RODRIGUEZ, Carmen-Lisset Flores MAURIZ, Antonius Jeroen Adriaan VAN MARIS, Jacob C. HARRISON
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Publication number: 20150031076Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: ApplicationFiled: October 2, 2014Publication date: January 29, 2015Inventors: Hubertus Johannes Marie OP DEN CAMP, Harry Ramanoedj HARHANGI, Christiaan VAN DER DRIFT, Jacobus Thomas PRONK
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Publication number: 20150010943Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include, e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.Type: ApplicationFiled: June 24, 2014Publication date: January 8, 2015Inventors: Aaron Adriaan WINKLER, Sipko Maarten KUYPER, Wilhelmus Theodorus Antonius Maria DE LAAT, Johannes Pieter VAN DIJKEN, Jacobus Thomas PRONK
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Publication number: 20140295514Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: ApplicationFiled: February 25, 2014Publication date: October 2, 2014Applicant: TECHNISCHE UNIVERSITEIT DELFTInventors: Jacobus Thomas PRONK, Antonius Jeroen Adriaan VAN MARIS, Victor Gabriel GUADALUPE MEDINA
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Patent number: 8795998Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: GrantFiled: July 23, 2010Date of Patent: August 5, 2014Assignee: Technische Universiteit DelftInventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
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Patent number: 8790903Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include. e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.Type: GrantFiled: September 2, 2011Date of Patent: July 29, 2014Assignee: DSM IP Assets B.V.Inventors: Aaron Adriaan Winkler, Sipko Maarten Kuyper, Wilhelmus Theordorus Antonius Maria De Laat, Johannes Pieter Van Dijken, Jacobus Thomas Pronk
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Publication number: 20130273601Abstract: The present invention relates to a yeast cell comprising one or more exogenous genes of a pentose metabolic pathway non-native to the yeast cell wherein the yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell. The invention further relates to pentose and glucose fermenting yeast cell that is capable of simultaneous pentose and glucose consumption.Type: ApplicationFiled: October 11, 2011Publication date: October 17, 2013Applicant: DSM IP ASSETS B.V.Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
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Publication number: 20130236932Abstract: The invention relates to a polypeptide having a mutation at one or more position corresponding to T219 of SEQ ID NO: 55, wherein the polypeptide has at least 50% sequence identity with SEQ ID NO: 55, and wherein the polypeptide has permease activity.Type: ApplicationFiled: October 11, 2011Publication date: September 12, 2013Applicant: DSM IP ASSETS B.V.Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paul Klaassen, Rene Marcel De Jong
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Patent number: 8367396Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: GrantFiled: November 14, 2011Date of Patent: February 5, 2013Assignee: DSM IP Assets B.V.Inventors: Hubertus Johannes Marie Op Den Camp, Harry Ramanoedj Harhangi, Christiaan Van Der Drift, Jacobus Thomas Pronk
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Publication number: 20120225451Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include. e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.Type: ApplicationFiled: September 2, 2011Publication date: September 6, 2012Applicant: Technische Universiteit DelftInventors: Aaron Adriaan WINKLER, Sipko Maarten Kuyper, Wilhelmus Theodorus Antonius Maria De Laat, Johannes Pieter Van Dijken, Jacobus Thomas Pronk
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Publication number: 20120208231Abstract: The invention relates to an eukaryotic cell expressing nucleotide sequences encoding the ara A, ara B and ara D enzymes whereby the expression of these nucleotide sequences confers on the cell the ability to use L-arabinose and/or convert L-arabinose into L-ribulose, and/or xylulose 5-phosphate and/or into a desired fermentation product such as ethanol. Optionally, the eukaryotic cell is also able to convert xylose into ethanol.Type: ApplicationFiled: January 6, 2012Publication date: August 16, 2012Applicant: DSM IP ASSETS B.V.Inventors: Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Hendrik Wouter WISSELINK, Johannes Pieter VAN DIJKEN, Aaron Adriaan WINKLER, Johannes Hendrik DE WINDE
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Publication number: 20120064607Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: ApplicationFiled: November 14, 2011Publication date: March 15, 2012Applicant: C5 Yeast Company B.VInventors: Hubertus Johannes Marie OP DEN CAMP, Harry Ramanoedj Harhangi, Christiaan Van Der Drift, Jacobus Thomas Pronk
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Patent number: 8058040Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: GrantFiled: October 15, 2009Date of Patent: November 15, 2011Assignee: C5 Yeast Company B.V.Inventors: Hubertus Johannes Marie Op Den Camp, Harry Ramanoedj Harhangi, Christiaan Van Der Drift, Jacobus Thomas Pronk
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Publication number: 20110275130Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.Type: ApplicationFiled: July 23, 2010Publication date: November 10, 2011Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
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Patent number: 8034591Abstract: The present invention relates to further genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers the host cell the ability of isomerizing xylose to xylulose. The further genetic modifications are aimed at improving the efficiency of xylose metabolism and include e.g. reduction of unspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.Type: GrantFiled: July 15, 2005Date of Patent: October 11, 2011Assignee: Techische Universiteit DelftInventors: Aaron Adriaan Winkler, Sipko Maarten Kuyper, Wilhelmus Theodorus Antonius Maria De Laat, Johannes Pieter Van Dijken, Jacobus Thomas Pronk
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Publication number: 20110104736Abstract: The present invention relates to a method for selecting a strain of an organism capable of improved consumption of a mixed substrate comprising two or more carbon sources as compared to a reference strain of the organism, which method comprises: growing a population of the reference strain of the organism in the presence of the two or more carbon sources, wherein the number of generations of growth of the said population on each of the said carbon sources is at least about 50% of the number of generations of growth on the carbon source most preferred by the organism; and selecting the resulting strain of the organism, thereby to select a strain of the organism capable of improved consumption of a mixed substrate comprising the two or more carbon sources as compared to the reference strain of the organism. The invention also relates to strains of organisms selected using such a method.Type: ApplicationFiled: March 10, 2009Publication date: May 5, 2011Inventors: Jacobus Thomas Pronk, Antonius Antonius Maris Van, Hendrik Wouter Wisselink
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Publication number: 20110045559Abstract: The present disclosure relates to modified yeast, wherein the yeast has reduced pyruvate decarboxylase polypeptide (PDC) activity and methods of using such yeast to produce malic and/or succinic acid.Type: ApplicationFiled: May 19, 2008Publication date: February 24, 2011Inventors: Aaron Adriaan Winkler, Abraham Frederik De Hulster, Johannes Pieter Van Dijken, Jacobus Thomas Pronk, Joshua Trueheart, Kevin T. Madden, Jacob C. Harrison, Carlos Gancedo Rodriguez, Carmen-Lisset Flores Mauriz