Abstract: The present invention is related to a method for inducing in vitro the differentiation of stem cells or somatic cells towards germinal cells, comprising the following steps: cultivating said stem cells or somatic cells in a medium allowing their differentiation, and collecting the germinal cells from the culture medium, wherein said cells are cells transformed with an exogenous genetic construction comprising at least a Vasa gene. Germinal cells such as obtained and chimeric animals are also an object of this invention.

Abstract: The present invention relates to a novel combination of avian cell markers which make it possible to characterize the said cells according to their phenotype, whether they are StX cells, stem cells or germ cells. The present invention also relates to a method for characterizing avian cells with the said markers, and to a method for culturing avian cells, in which the cells are characterized by the method according to the invention.

Abstract: The present invention relates to a method for preparing differentiated avian cells from stem cells in culture. Genes involved in maintaining the pluripotency of avian stem cells were identified and cloned. By inhibiting the expression of these genes in stem cells, the latter lose their pluripotency characteristics and enter into differentiation. These differentiated cells obtained in vitro can serve as host cells for pathogens, in particular viruses, and can thus be used for the production of antiviral vaccines.

Abstract: The invention concerns modified avian ES cells, specifically expressing an exogenous gene when they have a pluripotent character. The invention also concerns a nucleic acid and a polypeptide specifically expressed in pluripotent avian cells, and methods for detecting the pluripotent character of cells using said nucleic acid and polypeptide.

Abstract: A culture medium for avian embryonic cells and an avian cell culture medium is disclosed. The culture medium is characterized in that it has elements complementary to avian embryonic cells. The complementary elements include insulin-like growth factors and stem cell growth factors. The medium is substantially free of active retinoic acid. A method of culturing avian embryonic cells and the resulting products are disclosed.

Abstract: The invention concerns the use of an avian cell for producing an exogenous protein of interest in an animal belonging to the avian species, said cell being transformed by an expression vector comprising the gene coding for said protein, said cell being introduced in the sub-germinal cavity of an embryo, the blood stream of the embryo or being used as nucleus source for nuclear transfer into an avian egg enucleated or not, or whereof the chromosomes have been destroyed.

Abstract: The invention features non-transformant immortal avian cells, in particular derived from avian tissues, i.e., other than blood or hematopoietic cells, particularly fibroblasts and epithelial cells, for instance of embryos. The avian cells are immortalized by the SV40 T+t gene in the dependence of the MTI promoter. In particular they integrate the pDAMT vector.

Abstract: The invention features non-transformant immortal avian cells, in particular derived from avian tissues, i.e., other than blood or hematopoietic cells, particularly fibroblasts and epithelial cells, for instance of embryos. The avian cells are immortalized by the SV40 T+t gene in the dependence of the MTI promoter. In particular they integrate the pDAMT vector.

Abstract: The invention concerns modified avian ES cells, specifically expressing an exogenous gene when they have a pluripotent character. The invention also concerns a nucleic acid and a polypeptide specifically expressed in pluripotent avian cells, and methods for detecting the pluripotent character of cells using said nucleic acid and polypeptide.

Abstract: A promoter-free nucleic acid construct, includes a selection sequence and a sequence coding for a protein of interest distinct from the selection sequence, the coding sequence being preceded upstream by a sequence enabling its translation by ribosomes and the uses of the construct in the fields of biology and medicine.

Abstract: The invention features apoptosis-resistant, non-transformant immortalised avian cells, in particular, avian tissues, i.e., other than blood or haematopoietic cells, particularly fibroblasts and epithelial cells, for instance embryos.

Abstract: The invention features apoptosis-resistant, non-transformant immortalised avian cells, in particular, avian tissues, i.e., other than blood or haematopoietic cells, particularly fibroblasts and epithelial cells, for instance embryos.

Abstract: A culture medium for avian embryonic cells and an avian cell culture medium is disclosed. The culture medium is characterized in that it has elements complementary to avian embryonic cells. The complementary elements are cytokines, fibroblast growth factors, insulin-like growth factors or stem cell growth factors. The medium is substantially free of active retinoic acid. A method for culturing avian embryonic cells, and the resulting products, are also disclosed.

Abstract: A culture medium for avian totipotential embryonic cells comprising an avian cell culture medium is disclosed. The culture medium is characterised in that it comprises elements complementary to said avian cell culture medium, the complementary elements being selected from the group which comprises cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors, and in that it is substantially free of active retinoic acid. A method for culturing avian totipotential embryonic cells, and the resulting products, are disclosed.

Abstract: The invention features non-transformant immortal avian cells, in particular derived from avian tissues, i.e., other than blood or hematopoietic cells, particularly fibroblasts and epithelial cells, for instance of embryos. The avian cells are immortalized by the SV40 T+t gene in the dependence of the MTI promoter. In particular they integrate the pDAMT vector.

Abstract: A culture medium for avian totipotential embryonic cells comprising an avian cell culture medium is disclosed. The culture medium is characterised in that it comprises elements complementary to said avian cell culture medium, the complementary elements being selected from the group which comprises cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors, and in that it is substantially free of active retinoic acid. A method for culturing avian totipotential embryonic cells, and the resulting products, are also disclosed.

Abstract: The invention features non-transformant immortal avian cells, in particular derived from avian tissues, i.e. other than blood or haemotopoietic cells, particularly fibroblasts and epithelial cells, for instance of embryos. The avian cells are immortalized by the SV40 T+t gene in the dependence of the MTI promoter. In particular they integrate the pDAMT vector.

Abstract: The invention features apoptosis-resistant, non-transformant immortalised avian cells, in particular, avian tissues, i.e., other than blood or haematopoietic cells, particularly fibroblasts and epithelial cells, for instance embryos.

Abstract: The invention features apoptosis-resistant, non-transformant immortalised avian cells, in particular, avian tissues, i.e., other than blood or haematopoietic cells, particularly fibroblasts and epithelial cells, for instance embryos.

Abstract: A culture medium for culturing chicken embryonic stem (ES) cells is disclosed. The culture medium is used for supporting avian ES cell cultures. The components of the avian ES cell media include cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors and anti-retinoic acid antibody. The culture medium is substantially free of active retinoic acid. A method for culturing avian ES cells and the resulting products are also disclosed.