Abstract: Methods and devices for sealing a storage vessel or other apparatus are provided. A piston moves from a first position to a second position within a body to compress a sealing element, such as a metallic ring. One or more attachment elements connect the piston to the body. A removable pipe can be used for pump-out.

Abstract: Disclosed herein are methods and compositions for a new way to separate biologics using coated pegboards—plastic plates affixed with an array of 96-pegs which align with the wells of a traditional 96-well plate used in bench top experiments. The ends of the pegs are conjugated to various compounds which are able to bind tags on engineered proteins (ex. Ni-NTA would bind a His-Tag on an engineered protein). These pegs extend into plate wells to specifically bind the tagged protein from the cell lysate which contains hundreds of other proteins. The pegboard, now laden with the protein of interest (POI), may then be dipped into sequential buffers to wash away potential non-specifically bound proteins to purify the POI.

Abstract: An apparatus for cleaning a surface, the apparatus including a body defining a chamber, an inlet for liquid flow into the chamber, an outlet for liquid flow from the chamber, a nozzle connected to the outlet for generating an output flow of liquid for cleaning a surface, an acoustic transducer associated with the body to introduce acoustic energy into the liquid within the chamber whereby the acoustic energy is present in the liquid flowing out of the nozzle, and a gas bubble generator for generating gas bubbles within the liquid flowing out of the nozzle.

Abstract: An apparatus for cleaning a surface, the apparatus including a body defining a chamber, an inlet for liquid flow into the chamber, an outlet for liquid flow from the chamber, a nozzle connected to the outlet for generating an output flow of liquid for cleaning a surface, an acoustic transducer associated with the body to introduce acoustic energy into the liquid within the chamber whereby the acoustic energy is present in the liquid flowing out of the nozzle, and a gas bubble generator for generating gas bubbles within the liquid flowing out of the nozzle.

Abstract: Disclosed herein is a novel, cofactor balanced, cell-free biocatalysis pathway to make diacids from formaldehyde or methanol that does not use ATP and produces no CO2. In another embodiment, disclosed herein is a novel C5/C6 (hydrolysate) utilization pathway that interplays with the cell making diacids from formaldehyde or methanol that does not use ATP, produces no CO2, and replaces ATP with (cheap) polyphosphate (no other cofactors needed). Using methods and compositions disclosed herein, no CO2 is produced and the pathways disclosed herein are cell-free. The combination of pathways, feedstocks and products disclosed herein is novel.

Abstract: Disclosed herein are compositions of non-naturally occurring enzymes to enable microbial syringol utilization with GcoAB.

Abstract: Disclosed herein are compositions of non-naturally occurring enzymes to enable microbial syringol utilization with GcoAB.

Abstract: A method of making metal nanoparticles comprising contacting metal with a diazonium compound substituted with an organic substituent and allowing said metal to react with the diazonium compound thereby forming metal nanoparticles wherein each of said metal nanoparticles comprises a metal core bonded to an outer layer of said organic substituent as well as nanoparticles made by that method.

Abstract: An apparatus for cleaning a surface, the apparatus including a body defining a chamber, an inlet for liquid flow into the chamber, an outlet for liquid flow from the chamber, a nozzle connected to the outlet for generating an output flow of liquid for cleaning a surface, an acoustic transducer associated with the body to introduce acoustic energy into the liquid within the chamber whereby the acoustic energy is present in the liquid flowing out of the nozzle, and a gas bubble generator for generating gas bubbles within the liquid flowing out of the nozzle.

Abstract: The apparatus includes a two-stage mixer formed within a single housing and including a first high shear mixer and a second low shear mixer. Plaster slurry is formed in the first mixer. A partition is laid on a first fixing sheet, a portion laid on a second fixing sheet, and the remainder discharged to the second mixer. In the second mixer, foam is added and the formed slurry is laid on the unformed slurry on the first fixing sheet. The unformed slurry in the second fixing sheet is brought to the formed slurry to join the product.

Abstract: A rotary position transducer comprises a sensor cooperating with a toothed wheel which has nominally evenly spaced teeth and which may, for instance, be mounted on the crankshaft of an internal combustion engine. The times at which the teeth pass the sensor are stored. The time intervals between consecutive teeth are calculated, for instance during fuel cut-off operation of the engine. An error in the angular spacing between consecutive teeth is calculated for the time interval between consecutive teeth, a time interval between further teeth disposed symmetrically about the consecutive teeth, and the nominal angular spacing between the further teeth.

Abstract: The apparatus includes a two-stage mixer formed within a single housing and including a first high shear mixer and a second low shear mixer. Plaster slurry is formed in the first mixer. A partition is laid on a first fixing sheet, a portion laid on a second fixing sheet, and the remainder discharged to the second mixer. In the second mixer, foam is added and the formed slurry is laid on the unformed slurry on the first fixing sheet. The unformed slurry in the second fixing sheen is brought to the formed slurry to join the product.

Abstract: Test equipment, for testing that an image processing apparatus is operating correctly, is coupled to the output port of the image processing apparatus. A predetermined group of signals corresponding to a portion of an image from the image processing apparatus is stored in a memory in the test equipment. The signals are then analysed either by an operator using a monitor or by electronic comparison of the signals with reference signals corresponding to the portion of the image, from a reference store.

Abstract: A process for binding pieces of cooked meat together to produce a unitary product by impregnating pieces of uncooked meat with an aqueous solution containing a source of phosphate ions, cooking the meat, coating the cooked meat with an edible, heat-settable, thermostable glue containing an emulsifier selected from the group consisting of mono-diglycerides derived from stearic acid and sodium stearoyl-2-lactylate, compacting the coated pieces of meat and heating the compacted pieces of meat to obtain a product which on reheating remains cohesive.

Abstract: A fruit product having a texture which on eating is sensed as non-uniform like that of soft centered fruits such as gooseberries and cherries is prepared by a process in which fruit pulp or puree admixed with an alginate or pectate sol is brought into contact with a dissolved calcium ion so that part of the sol is gelled and the fruit pulp or puree is distributed between a fluid or plastic core of alginate or pectate sol and a firm exterior of calcium alginate or pectate gel and the product is heated to prevent the core from becoming a firm gel.