Patents by Inventor James Bustillo

James Bustillo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    Publication number: 20160010149
    Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 ?m or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.
    Type: Application
    Filed: September 23, 2015
    Publication date: January 14, 2016
    Inventors: Jonathan M. ROTHBERG, James BUSTILLO, Mark James MILGREW, Jonathan SCHULTZ, David MARRAN, Todd REARICK, Kim L. JOHNSON
  • Sensor arrays and methods for making same
    Patent number: 9194840
    Abstract: A system includes a sensor including a sensor pad and includes a well wall structure defining a well operatively connected to the sensor pad. The sensor pad is associated with a lower surface of the well. The well wall structure defines an upper surface and a wall surface extending between the upper surface and the lower surface. The upper surface is defined by an upper buffer material having an intrinsic buffer capacity of at least 2×1017 groups/m2. The wall surface is defined by a wall material having an intrinsic buffer capacity of not greater than 1.7×1017 groups/m2.
    Type: Grant
    Filed: January 19, 2012
    Date of Patent: November 24, 2015
    Assignee: Life Technologies Corporation
    Inventors: Shifeng Li, James Bustillo, Wolfgang Hinz
  • Methods and apparatus for measuring analytes using large scale FET arrays
    Patent number: 9194000
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: November 24, 2015
    Assignee: Life Technologies Corporation
    Inventors: Jonathan M. Rothberg, Wolfgang Hinz, Kim L. Johnson, James Bustillo, John Leamon, Jonathan Schultz
  • METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS
    Publication number: 20150329904
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
    Type: Application
    Filed: May 4, 2015
    Publication date: November 19, 2015
    Inventors: Jonathan M. ROTHBERG, Wolfgang Hinz, Kim L. Johnson, James Bustillo
  • Methods and apparatus for measuring analytes using large scale FET arrays
    Patent number: 9134269
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: September 15, 2015
    Assignee: Life Technologies Corporation
    Inventors: Jonathan Rothberg, Wolfgang Hinz, Kim Johnson, James Bustillo
  • Chemical sensors with consistent sensor surface areas
    Patent number: 9128044
    Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A material defines an opening extending to the upper surface of the floating gate conductor. The material comprises a first dielectric underlying a second dielectric. A conductive element contacts the upper surface of the floating gate conductor and extends a distance along a sidewall of the opening, the distance defined by a thickness of the first dielectric.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: September 8, 2015
    Assignee: Life Technologies Corporation
    Inventors: Keith G. Fife, Jordan Owens, Shifeng Li, James Bustillo
  • Chemical sensor with sidewall sensor surface
    Patent number: 9116117
    Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor. A material defines an opening overlying the floating gate conductor. The material comprises a conductive element having an inner surface defining a lower portion of a sidewall of the opening. A dielectric is on the conductive element and has an inner surface defining an upper portion of the sidewall.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: August 25, 2015
    Assignee: Life Technologies Corporation
    Inventors: Keith G. Fife, Jordan Owens, Shifeng Li, James Bustillo
  • INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    Publication number: 20150197797
    Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 ?m or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.
    Type: Application
    Filed: January 19, 2015
    Publication date: July 16, 2015
    Inventors: Jonathan M. ROTHBERG, James BUSTILLO, Mark James MILGREW, Jonathan SCHULTZ, David MARRAN, Todd REARICK, Kim L. JOHNSON
  • INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    Publication number: 20150160154
    Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 ?m or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.
    Type: Application
    Filed: January 21, 2015
    Publication date: June 11, 2015
    Inventors: Jonathan M. ROTHBERG, James Bustillo, Mark James Milgrew, Jonathan Schultz, David Marran, Todd Rearick, Kim L. Johnson
  • METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS
    Publication number: 20150126378
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.
    Type: Application
    Filed: October 22, 2014
    Publication date: May 7, 2015
    Inventors: JONATHAN M. ROTHBERG, WOLFGANG HINZ, KIM L. JOHNSON, JAMES BUSTILLO, JOHN LEAMON, JONATHAN SCHULTZ
  • High density sensor array without wells
    Patent number: 9023189
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: May 5, 2015
    Assignee: Life Technologies Corporation
    Inventors: Jonathan M. Rothberg, Wolfgang Hinz, Kim L. Johnson, James Bustillo
  • Chemically-sensitive field effect transistor based pixel array with protection diodes
    Patent number: 8994076
    Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
    Type: Grant
    Filed: September 7, 2012
    Date of Patent: March 31, 2015
    Assignee: Life Technologies Corporation
    Inventors: Mark Milgrew, James Bustillo, Todd Rearick
  • CHEMICAL SENSOR WITH SIDEWALL SPACER SENSOR SURFACE
    Publication number: 20150064829
    Abstract: In one implementation, a chemical sensor is described. The chemical sensor includes chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A dielectric material defines an opening extending to the upper surface of the floating gate conductor. A conductive sidewall spacer is on a sidewall of the opening and contacts the upper surface of the floating gate conductor.
    Type: Application
    Filed: November 17, 2014
    Publication date: March 5, 2015
    Inventors: Keith G. FIFE, James BUSTILLO, Jordan OWENS
  • Chemical sensor with sidewall spacer sensor surface
    Patent number: 8963216
    Abstract: In one implementation, a chemical sensor is described. The chemical sensor includes chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A dielectric material defines an opening extending to the upper surface of the floating gate conductor. A conductive sidewall spacer is on a sidewall of the opening and contacts the upper surface of the floating gate conductor.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: February 24, 2015
    Assignee: Life Technologies Corporation
    Inventors: Keith Fife, James Bustillo, Jordan Owens
  • Integrated sensor arrays for biological and chemical analysis
    Patent number: 8936763
    Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 ?m or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.
    Type: Grant
    Filed: October 22, 2009
    Date of Patent: January 20, 2015
    Assignee: Life Technologies Corporation
    Inventors: Jonathan Rothberg, James Bustillo, Mark Milgrew, Jonathan Schultz, David Marran, Todd Rearick, Kim Johnson
  • Chemical Sensor Array Having Multiple Sensors Per Well
    Publication number: 20140364320
    Abstract: In one embodiment, a device is described. The device includes a material defining a reaction region. The device also includes a plurality of chemically-sensitive field effect transistors have a common floating gate in communication with the reaction region. The device also includes a circuit to obtain respective output signals from the chemically-sensitive field effect transistors indicating an analyte within the reaction region.
    Type: Application
    Filed: June 2, 2014
    Publication date: December 11, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jonathan M. ROTHBERG, Keith G. FIFE, James BUSTILLO, James OWENS
  • Chemical sensor with protruded sensor surface
    Patent number: 8841217
    Abstract: In one implementation, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A dielectric material defines an opening extending to the upper surface of the floating gate conductor. A conductive element on a sidewall of the opening and extending over an upper surface of the dielectric material.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: September 23, 2014
    Assignee: Life Technologies Corporation
    Inventors: Keith Fife, James Bustillo, Jordan Owens
  • CHEMICAL SENSOR WITH SIDEWALL SENSOR SURFACE
    Publication number: 20140264469
    Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor. A material defines an opening overlying the floating gate conductor. The material comprises a conductive element having an inner surface defining a lower portion of a sidewall of the opening. A dielectric is on the conductive element and has an inner surface defining an upper portion of the sidewall.
    Type: Application
    Filed: March 5, 2014
    Publication date: September 18, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Keith G. FIFE, Jordan OWENS, Shifeng LI, James BUSTILLO
  • CHEMICAL SENSOR WITH PROTRUDED SENSOR SURFACE
    Publication number: 20140264466
    Abstract: In one implementation, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A dielectric material defines an opening extending to the upper surface of the floating gate conductor. A conductive element on a sidewall of the opening and extending over an upper surface of the dielectric material.
    Type: Application
    Filed: March 13, 2013
    Publication date: September 18, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Keith G. Fife, James Bustillo, Jordan Owens
  • CHEMICAL SENSORS WITH CONSISTENT SENSOR SURFACE AREAS
    Publication number: 20140264470
    Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A material defines an opening extending to the upper surface of the floating gate conductor. The material comprises a first dielectric underlying a second dielectric. A conductive element contacts the upper surface of the floating gate conductor and extends a distance along a sidewall of the opening, the distance defined by a thickness of the first dielectric.
    Type: Application
    Filed: March 5, 2014
    Publication date: September 18, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Keith G. FIFE, Jordan OWENS, Shifeng LI, James BUSTILLO