Patents by Inventor James Hickman
James Hickman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9650606Abstract: The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.Type: GrantFiled: June 9, 2014Date of Patent: May 16, 2017Assignee: University of Central Florida Research Foundation, Inc.Inventors: James Hickman, Mainak Das
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Patent number: 9489474Abstract: The invention provides a model for generating predicted action potentials of an electrically active cell. The disclosed model includes three operatively coupled submodels. A first submodel contains Hodgkin-Huxley elements generating action potentials based on electrical equivalent circuits. A second submodel is based on reaction kinetics of cell metabolism and is operatively coupled with the first submodel. A third submodel is based on Boolean dynamics representing signaling and associated cellular processes and is operatively coupled with the first and second submodels. The invention includes storing a library of calculated action potentials and associated cellular parameters generated by the model, applying a stimulus to the electrically active cell in vitro so that the cell generates an action potential; and comparing the cell-generated action potential with those stored in the library, wherein a match is predictive of the cellular point of action of the applied stimulus according to the parameters stored.Type: GrantFiled: February 7, 2011Date of Patent: November 8, 2016Assignee: University of Central Florida Research Foundation, Inc.Inventors: James Hickman, Peter Molnar, Frank Sommerhage, Jonathan Ernest Hood, Jerry Jenkins
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Publication number: 20160305927Abstract: The invention provides a culture of patterned cardiomyocytes, the culture including a support substrate bearing a multielectrode array, a negative surface resistant to cell attachment and deposited on the support substrate covering the multi-electrode array, a pattern ablated on the negative surface, a positive surface promoting cell attachment deposited on the pattern ablated on the negative surface; and cardiomyocytes adherent to the positive surface and growing aligned along the pattern ablated on the negative surface. The invention also includes a method of making the culture of patterned cardiomyocytes and a method of in vitro method of testing the effect of a compound on cardiac function by measuring electrical output from the patterned cardiomyocytes.Type: ApplicationFiled: June 23, 2016Publication date: October 20, 2016Inventors: Peter Molnar, Anupama Natarajan, Maria Stancescu, James Hickman
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Publication number: 20160267754Abstract: A non-contact voltage detector for detecting and indicating voltage. The non-contact voltage detector includes a housing having a voltage detection probe and plurality of light sources. The housing further includes an integral power source that is rechargeable via an external power source coupled to a user input port. The power source provides electrical energy to the non-contact voltage detector that further includes a controller and control circuit that is operably connected to at least one light source. The controller and control circuit can detect electrical energy at the user input port, detect the charge state of the integral power source, charge the integral power source, and emit one or more charge state light indicators when the state of the integral power source changes. In addition, the light sources can indicate the presence of voltage at the probe and can be independently activated by one or more switches.Type: ApplicationFiled: March 11, 2016Publication date: September 15, 2016Inventor: James Hickman
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Patent number: 9404140Abstract: The invention provides a culture of patterned cardiomyocytes, the culture including a support substrate bearing a multielectrode array, a negative surface resistant to cell attachment and deposited on the support substrate covering the multi-electrode array, a pattern ablated on the negative surface, a positive surface promoting cell attachment deposited on the pattern ablated on the negative surface; and cardiomyocytes adherent to the positive surface and growing aligned along the pattern ablated on the negative surface. The invention also includes a method of making the culture of patterned cardiomyocytes and a method of in vitro method of testing the effect of a compound on cardiac function by measuring electrical output from the patterned cardiomyocytes.Type: GrantFiled: November 3, 2010Date of Patent: August 2, 2016Assignee: The University of Central Florida Research Foundation, Inc.Inventors: Peter Molnar, Anupama Natarajan, Maria Stancescu, James Hickman
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Patent number: 9267936Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more human muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a human muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.Type: GrantFiled: August 6, 2014Date of Patent: February 23, 2016Assignee: University of Central Florida Research FoundationInventors: James Hickman, Xiufang Guo
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Publication number: 20160041150Abstract: Disclosed herein are devices comprising one or more cantilevers comprising one or more neuromuscular junctions formed by a co-culture of myotubes and motoneurons. Disclosed herein are methods of using the disclosed devices comprising one or more cantilevers. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.Type: ApplicationFiled: August 7, 2015Publication date: February 11, 2016Inventors: James Hickman, Alexander Smith, Christopher Long, Kristen Pirozzi
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Publication number: 20150369791Abstract: In an aspect, disclosed herein are physiological devices and systems, and components thereof, used to evaluate cardiac parameters and arrhythmogenic mechanisms. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.Type: ApplicationFiled: January 30, 2014Publication date: December 24, 2015Inventors: James HICKMAN, Maria STANCESCU, Peter MOLNAR, Christopher LONG, Christopher McAleer
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Publication number: 20140349884Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more human muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a human muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.Type: ApplicationFiled: August 6, 2014Publication date: November 27, 2014Applicant: University of Central Florida Research Foundation, Inc.Inventors: James Hickman, Xiufang Guo
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Publication number: 20140302599Abstract: The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.Type: ApplicationFiled: June 9, 2014Publication date: October 9, 2014Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.Inventors: James Hickman, Mainak DAS
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Patent number: 8835168Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more human muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a human muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.Type: GrantFiled: May 6, 2011Date of Patent: September 16, 2014Assignee: University of Central Florida Research Foundation, Inc.Inventors: James Hickman, Xiufang Guo
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Patent number: 8828721Abstract: The present invention provides a method of inducing myelination of isolated motoneurons by preparing a non-biological substrate having thereon a covalently attached monolayer of DETA; depositing isolated motoneurons on the substrate in a defined serum-free medium; plating isolated Schwann cells cultured in the defined serum-free medium onto the motoneurons, thereby initiating a co-culture; and passaging the co-culture as necessary into fresh, defined serum-free medium supplemented with L-ascorbic acid at least until the motoneurons form Nodes of Ranvier indicative of myelination. The invention also includes a method of testing for new drugs effective in demyelinating diseases. Additionally, cellular products provided by the invention include an isolated motoneurons myelinated or remyelinated in vitro according to the methods disclosed.Type: GrantFiled: May 27, 2010Date of Patent: September 9, 2014Assignee: University of Central Florida Research Foundation, Inc.Inventors: James Hickman, John Rumsey
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Publication number: 20140206028Abstract: Disclosed are compositions and methods of making stable electrically active adult neurons from adult neural tissue. The disclosed compositions can be used with microelectrode arrays in vitro to represent in vivo neural function for drug discovery and for studying neuronal degenerative diseases, neuronal development, and neuronal regeneration.Type: ApplicationFiled: May 17, 2012Publication date: July 24, 2014Applicant: University of Central Florida Research Foundation, Inc.Inventors: James Hickman, Darin Edwards
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Publication number: 20130115694Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more human muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a human muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.Type: ApplicationFiled: May 6, 2011Publication date: May 9, 2013Applicant: University of Central Florida Research Foundation, Inc.Inventors: James Hickman, Xiufang Guo
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Publication number: 20130096888Abstract: The invention provides a model for generating predicted action potentials of an electrically active cell. The disclosed model includes three operatively coupled submodels. A first submodel contains Hodgkin-Huxley elements generating action potentials based on electrical equivalent circuits. A second submodel is based on reaction kinetics of cell metabolism and is operatively coupled with the first submodel. A third submodel is based on Boolean dynamics representing signaling and associated cellular processes and is operatively coupled with the first and second submodels. The invention includes storing a library of calculated action potentials and associated cellular parameters generated by the model, applying a stimulus to the electrically active cell in vitro so that the cell generates an action potential; and comparing the cell-generated action potential with those stored in the library, wherein a match is predictive of the cellular point of action of the applied stimulus according to the parameters stored.Type: ApplicationFiled: February 7, 2011Publication date: April 18, 2013Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.Inventors: James Hickman, Peter Molnar, Frank Sommerhage, Jonathan Hood, Jerry Jenkins
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Patent number: 8272018Abstract: A method of creating an audio/video stream comprises multiplexing a video component, an audio component, a content reference identifier, and a semantic data packet containing information on the relationship between the video component and the content reference identifier. Apparatus for receiving the audio/video stream, and apparatus for transmitting the audio/video stream are disclosed.Type: GrantFiled: January 14, 2003Date of Patent: September 18, 2012Assignee: Koninklike Philips Electronics N.V.Inventors: Alexis Stephen Richard Ashley, Andrew James Hickman, Octavius John Morris
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Patent number: 8046808Abstract: A method of creating an audio-video stream comprises multiplexing a video component, an audio component, a content reference identifier, and a semantic data packet containing information on the relationship between the video component and the content reference identifier. Apparatus for receiving the audio-video stream, and apparatus for transmitting the audio/video stream are disclosed.Type: GrantFiled: May 23, 2005Date of Patent: October 25, 2011Assignee: Koninklijke Philips Electronics N.V.Inventors: Alexis Stephen Richard Ashley, Andrew James Hickman, Octavius John Morris
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Publication number: 20110250682Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more rat muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a rat muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.Type: ApplicationFiled: May 6, 2011Publication date: October 13, 2011Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.Inventors: JAMES HICKMAN, XIUFANG GUO, MERCEDES GONZALEZ, MARIA STANCESCU
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Patent number: 8032894Abstract: In embodiments, an implementation of a service oriented architecture is provided including an application service bus capable of approximating point-to-point performance by reducing the format transformation of application messages by way of relaying them in a native format when the message format of a consumer application and/or service provider application is supported by the service bus. Preferably, the service bus is capable of supporting multiple message formats and transport protocols and comprises a plurality of components including a Service Initiator module, a Service Terminus module, a Service Locator module, and a Transport module. The service bus provides logical isolation between a consumer application and a provider application by exposing a set of interfaces for relaying service request and service response messages between the applications.Type: GrantFiled: December 28, 2007Date of Patent: October 4, 2011Assignee: Aetna Inc.Inventors: Bruce Ian Lazaroff, Youyi Mao, Sean James Hickman, Peter Robert Nicholson
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Patent number: 7905621Abstract: An in-grade light fixture has a lamp compartment having an open upper end with a lens cover covering the open upper end. The lens cover may have a joint connection to permit travel from an open configuration to a closed configuration. A junction box is adjacent to the lens cover along an upper portion of the compartment. If a ballast is utilized for proper supply of power to the lamp, a ballast compartment is adjacent an open lower end of the lamp compartment. A hydraulic isolation chamber extends vertically downward from the junction box to the sealed ballast compartment. The hydraulic isolation chamber is filled with a potting material and prevents wicking of moisture into the ballast compartment.Type: GrantFiled: January 18, 2008Date of Patent: March 15, 2011Assignee: Genlyte Thomas Group, LLCInventors: James Hickman, Matthew Pressel, Michael Riebling, David Smith, Lew Waltz