Abstract: The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can be extracted from the standard.

Abstract: The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can be extracted from the standard.

Abstract: The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can he extracted from the standard.

Abstract: Nucleic acid sequence determination is a method whereby peaks in data traces representing the detection of labeled nucleotides are classified as either noise or specific nucleotides. Embodiments are described herein that formulate this classification as a graph theory problem whereby graph edges encode peak characteristics. The graph can then be traversed to find the shortest path. Various embodiments formulate the graph in such a way as to minimize computational time. In various cases it is desirable that such classification allow for the possibility of mixed bases in the nucleotide sequence. Embodiments are described herein that address the classification of mixed-bases. Embodiments are also described that detail methods and systems for processing the data in order to make the classification step robust and reliable.

Abstract: The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can he extracted from the standard.