Abstract: The present disclosure provides nucleic acid carriers comprising targeting agents, pharmaceutical compositions comprising the same, and methods of making and using the same.

Abstract: The present disclosure provides nucleic acid carriers comprising targeting agents, pharmaceutical compositions comprising the same, and methods of making and using the same.

Abstract: The present disclosure provides nucleic acid carriers comprising one or more layers comprising multiple monomers, wherein each monomer comprises: a first and a second oligonucleotide; wherein a central portion of each of the first and second oligonucleotides is complementary to each other, forming a double-stranded region, wherein the two terminal portions of the first oligonucleotide are not complementary to the two terminal portions of the second oligonucleotide, forming four terminal arms; and at least one targeting agent conjugated to at least one peripheral terminal arm, wherein when the at least one targeting agent is an miRNA. The present disclosure also provides pharmaceutical composition comprising any of the nucleic acid carriers described herein, and a pharmaceutically acceptable vehicle. The present disclosure also provides methods of inducing an immune response in an animal comprising administering to the animal any of the nucleic acid carriers described herein.

Abstract: The present invention relates to novel lateral flow devices using DNA dendrimers, and the methods for detecting an analyte using the lateral flow devices.

Abstract: The present invention relates to lateral flow devices and kits using DNA dendrimers, and to methods for detecting an analyte using the lateral flow devices and kits. More specifically, the DNA dendrimers may be dried on a location on the test device upstream from said test zone, before said test device is used.

Abstract: The present invention relates to novel lateral flow devices using DNA dendrimers, and the methods for detecting an analyte using the lateral flow devices.

Abstract: A composition for forming a protective coating over the surface of an assay substrate having an indicating agent capable of generating a detectable signal associated therewith, includes a protective coating forming material, and a delivery system for delivering the protective coating forming material in an amount sufficient to coat the surface of the assay substrate, wherein the delivery system evaporates from the surface of the assay substrate to form a protective coating at least substantially composed of the protective coating forming material that is at least substantially transparent to the detectable signal generated by the indicating agent. The present invention is further directed to a method for forming the protective coating over the surface of the assay substrate, and a substrate produced by such method.

Abstract: Methods are provided for blocking non-specific and specific hybridization of nucleic acid samples on a microarray. The method of the present invention comprises applying a blocking reagent to the microarray, wherein the blocking reagent comprises modified nucleotide bases, preferably LNA (Locked Nucleic Acid—modified bicyclic monomeric units with a 2′-O-4′-C methylene bridge). In further embodiments, the method includes applying a mixture including: a) a cDNA reagent obtained from mRNA of a target sample, the cDNA having a capture sequence; b) a dendrimer with a label for emitting a detectable signal and a second nucleotide sequence complementary to the capture sequence; and c) an blocking reagent containing LNA to a microarray, for producing a detectable signal from said label whereby a hybridization pattern is generated on the microarray.

Abstract: Disclosed are reagents for conducting quality control reactions on microarrays of nucleic acids and kits containing the reagents, along with directions for conducting the reactions with the components in the kits. Also disclosed are methods of preparing the kits as well as using them to conduct the quality control reactions. A preferred reagent embodiment is illustrated in FIG. 3.

Abstract: The present invention is directed to a method for determining the presence of a specific nucleotide sequence in a cDNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the cDNA reagent on a microarray through temperature cycling.

Abstract: The present invention is directed to a method for determining the presence of a specific nucleotide sequence in an RNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the RNA reagent on a microarray.