Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in eucaryotic cells. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide. In particular, a sequence coding for ubiquitin is joined to another polypeptide of interest providing for high yields of the fusion product.

Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in eucaryotic cells. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide. In particular, a sequence coding for ubiquitin is joined to another polypeptide of interest providing for high yields of the fusion product.

Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.

Abstract: Polynucleotide sequences which encode for human prepro insulin-like growth factors are provided. Such sequences are obtained from the human genome, typically by screening a cDNA library obtained from human liver cells. The polynucleotide sequences may be used for cloning and expression of insulin-like growth factors in suitable hosts, as well as for the production of DNA and RNA which may be used as hybridization probes.E. coli strains HB101(phigf1) and HB101(phigf2) were deposited at the ATCC on Jun. 8, 1984, and granted accession nos. 39729 and 39730, respectively.

Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S.

Abstract: A DNA having a base sequence coding for human epidermal growth factor has been synthesized in blocks, and cloned. Novel recombinant DNA transfer vectors containing said cloned DNA have been constructed. The codon usage of the sequence reflects the codon bias of yeast. The DNA sequence is useful for the large scale synthesis of human epidermal growth factor in yeast cells or microorganisms transformed by said recombinant DNA transfer vectors.

Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S.