Patents by Inventor James P. Wynn

James P. Wynn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Protein production in microorganisms of the phylum labyrinthulomycota
    Patent number: 10829794
    Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
    Type: Grant
    Filed: January 16, 2019
    Date of Patent: November 10, 2020
    Assignee: DSM IP Assets B.V.
    Inventors: Kirk E. Apt, James Casey Lippmeier, David Simpson, Jun Wang, James P. Wynn, Ross Zirkle
  • PROTEIN PRODUCTION IN MICROORGANISMS OF THE PHYLUM LABYRINTHULOMYCOTA
    Publication number: 20190211369
    Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
    Type: Application
    Filed: January 16, 2019
    Publication date: July 11, 2019
    Inventors: Kirk E. APT, James Casey LIPPMEIER, David SIMPSON, Jun WANG, James P. WYNN, Ross ZIRKLE
  • Production of high levels of DHA-containing biomass in microalgae using modified amounts of chloride and potassium
    Patent number: 9834799
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: December 5, 2017
    Assignee: DSM IP Assets B.V.
    Inventors: Paul W Behrens, John M Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • PROTEIN PRODUCTION IN MICROORGANISMS OF THE PHYLUM LABYRINTHULOMYCOTA
    Publication number: 20170145461
    Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
    Type: Application
    Filed: November 3, 2016
    Publication date: May 25, 2017
    Inventors: Kirk E. APT, James Casey LIPPMEIER, David SIMPSON, Jun WANG, James P. WYNN, Ross ZIRKLE
  • Polypeptide for improving protein production in microorganisms of the phylum Labyrinthulomycota
    Patent number: 9518102
    Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: December 13, 2016
    Assignee: DSM IP Assets B.V.
    Inventors: Kirk E. Apt, James Casey Lippmeier, David Simpson, Jun Wang, James P. Wynn, Ross Zirkle
  • Production of High Levels of DHA-Containing Biomass in Microalgae Using Modified Amounts of Chloride and Potassium
    Publication number: 20160108443
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Application
    Filed: December 18, 2015
    Publication date: April 21, 2016
    Inventors: Paul W. BEHRENS, John M. THOMPSON, Kirk APT, Joseph W. PFEIFER, James P. WYNN, James Casey LIPPMEIER, Jaouad FICHTALI, Jon HANSEN
  • Production of high levels of DHA in microalgae using modified amounts of chloride and potassium
    Patent number: 9249434
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: January 27, 2014
    Date of Patent: February 2, 2016
    Assignee: DSM IP Assets B.V.
    Inventors: Paul W Behrens, John M Thompson, Kirk Apt, Joseph W Pfeifer, James P Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • Production of omega-3 fatty acids from pythium species
    Patent number: 9074160
    Abstract: The present invention relates to the production of a triacylglycerol oil that is produced from a Pythium species of alga. In one embodiment, the algal species contains at least about 20 weight percent total lipids and contains at least about 10 weight percent of its total fatty acids as eicosapentaenoic acid (EPA) and less than about 5 weight percent of its total fatty acids as arachidonic acid (ARA). In another embodiment, the present invention relates to various methods to produce eicosapentaenoic acid (EPA) from a Pythium species of algae. In particular, Pythium irregulare can be utilized as a viable production organism.
    Type: Grant
    Filed: March 7, 2013
    Date of Patent: July 7, 2015
    Assignee: AlgiSys, LLC
    Inventors: Charles L. Roe, James P. Wynn
  • POLYPEPTIDE FOR IMPROVING PROTEIN PRODUCTION IN MICROORGANISMS OF THE PHYLUM LABYRINTHULOMYCOTA
    Publication number: 20150132803
    Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
    Type: Application
    Filed: November 19, 2014
    Publication date: May 14, 2015
    Inventors: Kirk E. APT, James Casey LIPPMEIER, David SIMPSON, Jun WANG, James P. WYNN, Ross ZIRKLE
  • Polynucleotide for improving protein production in microorganisms of the phylum labyrinthulomycota
    Patent number: 9012616
    Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: April 21, 2015
    Assignee: DSM IP Assets B.V.
    Inventors: Kirk E. Apt, James Casey Lippmeier, David Simpson, Jun Wang, James P. Wynn, Ross Zirkle
  • PRODUCTION OF OMEGA-3 FATTY ACIDS FROM PYTHIUM SPECIES
    Publication number: 20140256973
    Abstract: The present invention relates to the production of a triacylglycerol oil that is produced from a Pythium species of alga. In one embodiment, the algal species contains at least about 20 weight percent total lipids and contains at least about 10 weight percent of its total fatty acids as eicosapentaenoic acid (EPA) and less than about 5 weight percent of its total fatty acids as arachidonic acid (ARA). In another embodiment, the present invention relates to various methods to produce eicosapentaenoic acid (EPA) from a Pythium species of algae. In particular, Pythium irregulare can be utilized as a viable production organism.
    Type: Application
    Filed: March 7, 2013
    Publication date: September 11, 2014
    Applicant: Algysis LLC
    Inventors: Charles L. Roe, James P. Wynn
  • PRODUCTION OF HIGH LEVELS OF DHA IN MICROALGAE USING MODIFIED AMOUNTS OF CHLORIDE AND POTASSIUM
    Publication number: 20140142331
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Application
    Filed: January 27, 2014
    Publication date: May 22, 2014
    Inventors: Paul W. BEHRENS, John M. THOMPSON, Kirk APT, Joseph W. PFEIFER, James P. WYNN, James Casey LIPPMEIER, Jaouad FICHTALI, Jon HANSEN
  • PROTEIN PRODUCTION IN MICROORGANISMS OF THE PHYLUM LABYRINTHULOMYCOTA
    Publication number: 20140106402
    Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
    Type: Application
    Filed: December 17, 2013
    Publication date: April 17, 2014
    Applicant: DSM IP Assets B.V.
    Inventors: Kirk E. APT, James Casey LIPPMEIER, David SIMPSON, Jun WANG, James P. WYNN, Ross ZIRKLE
  • Production of high levels of DHA in microalgae using modified amounts of chloride and potassium
    Patent number: 8669090
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: March 11, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • Production of high levels of DHA in microalgae using modified amounts of chloride and potassium
    Patent number: 8663953
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: March 4, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • Protein production in microorganisms of the phylum labyrinthulomycota
    Patent number: 8637651
    Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: January 28, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Kirk E. Apt, James Casey Lippmeier, David Simpson, Jun Wang, James P. Wynn, Ross Zirkle
  • Production of high levels of DHA in microalgae using modified amounts of chloride and potassium
    Patent number: 8497100
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: July 30, 2013
    Assignee: DSM IP Assets B.V.
    Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • Production of high levels of DHA in microalgae using modified amounts of chloride and potassium
    Patent number: 8497099
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: July 30, 2013
    Assignee: DSM IP Assets B.V.
    Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • Production of High Levels of DHA in Microalgae Using Modified Amounts of Chloride and Potassium
    Publication number: 20120071677
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Application
    Filed: November 29, 2011
    Publication date: March 22, 2012
    Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • Methods for discouraging bacterial growth in culture medium
    Patent number: 8008050
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: April 20, 2007
    Date of Patent: August 30, 2011
    Assignee: Martek Biosciences Corporation
    Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen