Abstract: A modeling system determines a physical location of a device in a store and a virtual location in a virtual model of a store that corresponds to the physical location. The modeling system determines, from the virtual model, a subset of model data based on the virtual location. The model data comprises store location data applicable to the entire store, shelf data applicable to shelves in the store, and planogram data applicable to the shelves and products associated with the shelves. The subset of model data comprises the store location data, a subset of the shelf data applicable to at least a shelf within a predefined distance of the physical location of the device, and a subset of the planogram data applicable to at least the shelf and a product associated with the shelf. The modeling system generates a presentation of a scene based on the subset of model data.

Abstract: Methods and compositions are provided for amplifying a pool of oligonucleotides, such as dual guide oligonucleotide constructs comprising sequences encoding a first guide RNA segment and a sequence encoding a second guide RNA segment. An amplification mixture is formed comprising the pool of oligonucleotides, an amplification enzyme, deoxyribonucleotide triphosphates, and primers. The amplification mixture is thermocycled a sufficient number of times and under conditions to produce a library of oligonucleotide constructs. The present methods and compositions provide dual guide libraries, including libraries that are essentially free of scrambled library members.

Abstract: Provided herein is a method for fabricating transformable or transfectable molecules that includes an assembly reaction containing a variety of pre-made cassettes possessing ends that hybridize to one another, transforming or transfecting said molecules into a desired host cell and then selecting a transformed/transfected host cell containing plasmid molecules composed of said the cassettes. A kit for performing the method is also provided.

Abstract: The present subject matter relates to devices, systems, and methods for applying pesticides, herbicides, and other treatment chemicals to a surface. A roller application device for use in delivery of treatment chemicals to a landscape surface includes a handle assembly having a fluid connector configured to be connected to a treatment chemical source, and a roller applicator is connected to the handle assembly but configured to rotate about an axis. The handle assembly includes a fluid dispenser configured to deliver a treatment chemical from the treatment chemical source to the roller applicator, and the roller applicator is configured to absorb the treatment chemical in an outer surface of the roller applicator and transfer the treatment chemical to a surface upon contact with the surface.

Abstract: Provided herein is a method for fabricating transformable or transfectable molecules that includes an assembly reaction containing a variety of pre-made cassettes possessing ends that hybridize to one another, transforming or transfecting said molecules into a desired host cell and then selecting a transformed/transfected host cell containing plasmid molecules composed of said the cassettes. A kit for performing the method is also provided.

Abstract: Provided herein is a method for fabricating transformable or transfectable molecules that includes an assembly reaction containing a variety of pre-made cassettes possessing ends that hybridize to one another, transforming or transfecting said molecules into a desired host cell and then selecting a transformed/transfected host cell containing plasmid molecules composed of said the cassettes. A kit for performing the method is also provided.

Abstract: Methods and compositions are provided for amplifying a pool of oligonucleotides, such as dual guide oligonucleotide constructs comprising sequences encoding a first guide RNA segment and a sequence encoding a second guide RNA segment. An amplification mixture is formed comprising the pool of oligonucleotides, an amplification enzyme, deoxyribonucleotide triphosphates, and primers. The amplification mixture is thermocycled a sufficient number of times and under conditions to produce a library of oligonucleotide constructs. The present methods and compositions provide dual guide libraries, including libraries that are essentially free of scrambled library members.

Abstract: Provided herein is a method for fabricating transformable or transfectable molecules that includes an assembly reaction containing a variety of pre-made cassettes possessing ends that hybridize to one another, transforming or transfecting said molecules into a desired host cell and then selecting a transformed/transfected host cell containing plasmid molecules composed of said the cassettes. A kit for performing the method is also provided.

Abstract: A joist mounted storage system having a pair of saddles, each of which rest upon and about adjacent joists. Extending upwardly from the saddles are arms between which pulleys are suspended and supported for the purpose of raising and lowering objects for storage.

Abstract: A roofing hatchet having a magnetic striking face is hereby disclosed. A unique shouldered cap housing the magnet dampens the forces transferred from the striking by transferring the force directly to the hatchet head, bypassing the magnet, thereby preventing demagnetization typically caused by such repeated linear forces. Additional features include a cutting blade housing adapted to adjustably receive a variety of different commercially available utility knife blades, an adjustable gauge for gauging a distance from the bitted end of the hatchet, and a pulling notch for pulling fasteners from a surface. Unlike prior art hatchets, the handle of the inventive hatchet is angled, relative to the striking face, such that the force transfer from the hand of a user to a fastener being driven into a sloping surface is optimized.

Abstract: A mold assembly is made up of a nozzle tip and a nozzle tip receiving housing. The nozzle tip has an outwardly projecting shoulder and a threaded base and the housing has a hollow stem with an internal recess and a threaded socket for receiving the base of the nozzle tip. The assembly further includes a locking member having both internal and external projections. The locking member fits over the nozzle tip with the external projection of the locking member locating within the internal recess of the housing stem and with the outwardly projecting shoulder of the nozzle tip trapped by the internal projection of the locking member to block unthreading of the nozzle tip from the housing.

Abstract: A mold assembly is made up of a nozzle tip and a nozzle tip receiving housing. The nozzle tip has an outwardly projecting shoulder and a threaded base and the housing has a hollow stem with an internal recess and a threaded socket for receiving the base of the nozzle tip. The assembly further includes a locking member having both internal and external projections. The locking member fits over the nozzle tip with the external projection of the locking member locating within the internal recess of the housing stem and with the outwardly projecting shoulder of the nozzle tip trapped by the internal projection of the locking member to block unthreading of the nozzle tip from the housing.

Abstract: The present invention provides a mold with gas ejection of pieces formed in the mold. The mold includes a female body part and a male body part. The male body part includes a male molding surface with a surrounding plastic injection shut off face and a reciprocable gas controlling blow off pin slideably trapped within a pin recess at the shut off face. The pin has an enlarged bottom portion and a narrowed upper portion with a gas passage starting upwardly through the lower bottom portion into the upper portion and opening sideways through the pin which has a solid top surface above the sideways opening. The pin receiving recess has an undercut configuration including a small diameter upper region and a larger diameter base region with clearance for sliding of the pin in the recess. The clearance is at a minimum near the solid top surface of the pin and increases downwardly from the upper to the lower portion of the pin.

Abstract: The present invention provides in a stack mold with back to back centrally located mold cavities a multiple sprue bar arrangement including a pair of sprue bars balanced on opposite sides of the mold and connecting bridge across the sprue bars for interfacing to a plastic injector. At least one of the sprue bars provides a plastic flow path from the connecting bridge to the mold cavities.

Abstract: The present invention provides in a stack mold comprising a center mold part, first and second outside mold parts moveable at both sides of the center mold part, a rack and pinion gear arrangement to move the outside mold parts. The rack and pinion gear arrangement comprises a plurality of pinion gears located at the center mold part and including a drive gear and first and second follower gears driven off the drive gear. Also provided are first and second free ended racks extending in opposite directions from the first and second outside mold parts respectively in engagement with the first and second follower gears. These follower gears reach above and below the drive gear and the first follower gear is offset to the side of the drive gear opposite that to which the free end of the first rack extends while the second follower gear is offset to the side of the drive gear opposite that to which the free end of the second rack extends.